Inversion of both gating polarity and CO2 sensitivity of voltage gating with D3N mutation of Cx50

American Journal of Physiology - Cell Physiology

Volumn 288, Issue 6 57-6, 2005, Pages

  Peracchia, Camillo ^a   Peracchia, Lillian L ^a  

^a UNIVERSITY OF ROCHESTER SCHOOL OF MEDICINE AND DENTISTRY   (United States)

Author keywords

Cell communication; Channel gating; Chemical gating; Gap junctions; Lens; Xenopus oocytes

Indexed keywords

ASPARAGINE; ASPARTIC ACID; CARBON DIOXIDE; CONNEXIN 50; GAP JUNCTION PROTEIN; UNCLASSIFIED DRUG; EYE PROTEIN;

ACIDIFICATION; ANIMAL CELL; ARTICLE; CELL COMMUNICATION; CELL MEMBRANE CONDUCTANCE; CELL POLARITY; CHANNEL GATING; CONTROLLED STUDY; FEMALE; GAP JUNCTION; NONHUMAN; OOCYTE; PRIORITY JOURNAL; VOLTAGE CLAMP; XENOPUS; ANIMAL; CELL MEMBRANE POTENTIAL; GENE EXPRESSION; GENETICS; MOUSE; PHYSIOLOGY; POINT MUTATION; TRANSGENIC ORGANISM; XENOPUS LAEVIS;

ANIMALS; CARBON DIOXIDE; CONNEXINS; EYE PROTEINS; GENE EXPRESSION; ION CHANNEL GATING; MEMBRANE POTENTIALS; MICE; OOCYTES; ORGANISMS, GENETICALLY MODIFIED; POINT MUTATION; XENOPUS LAEVIS;

EID: 19644381349     PISSN: 03636143     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpcell.00348.2004     Document Type: Article

References (42)

1. Anumonwo JMB, Taflet SM, Gu H, Chanson M, Moreno AP, and Delmar M. The carboxyl terminal domain regulates the unitary conductance and voltage dependence of connexin40 gap junction channels. Circ Res 88: 666-673, 2001.
2. s connexin knockout mice. J Gen Physiol 118: 447-456, 2001.
3. Barrio LC, Suchyna T, Bargiello T, Xu LX, Roginski RS, Bennett MVL, and Nicholson BJ. Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage. Proc Natl Acad Sci USA 88: 8410-8414, 1991.
4. Beahm DL and Hall JE. Hemichannel and junctional properties of connexin 50. Biophys J 82: 2016-2031, 2004.
5. 2-sensitive and voltage-sensitive. Biophys J 72: 2137-2142, 1997.
6. Bukauskas FF, Bukauskiene A, Verselis VK, and Bennett MVL. Coupling asymmetry of heterotypic connexin 45/connexin 43-EGFP gap junctions: Properties of fast and slow gating mechanisms. Proc Natl Acad Sci USA 99: 7113-7118, 2002.
7. Cicirelli MF, Robinson KR, and Smith LD. Internal pH of oocytes: a study of the mechanism and role of pH changes during maturation. Dev Biol 100: 133-146, 1983.
8. Harris AL. Emerging issues of connexin channels: biophysics fills the gap. Q Rev Biophys 34: 325-472, 2001.
9. Lin JS, Eckert R, Kistler J, and Donaldson P. Spatial differences in gap junction gating in the lens are a consequence of connexin cleavage. Eur J Cell Biol 76: 246-250, 1998.
10. Loewenstein WR. Permeability of membrane junctions. Ann NY Acad Sci 137: 441-472, 1966.
11. Oh S, Abrams CK, Verselis VK, and Bargiello TA. Stoichiometry of transjunctional voltage-gating polarity reversal by a negative charge substitution in the amino terminus of a connexin32 chimera. J Gen Physiol 116: 13-31, 2000.
12. Peracchia C. Chemical gating of gap junction channels. Roles of calcium, pH and calmodulin. Biochim Biophys Acta 1662: 61-80, 2004.
13. 2 sensitivity of voltage gating and gating polarity of gap junction channels-connexin40 and its COOH-terminus truncated mutant. J Membr Biol 200: 105-113, 2004.
14. Peracchia C, Lazrak A, and Peracchia LL. Molecular models of channel interaction and gating in gap junctions. In: Handbook of Membrane Channels-Molecular and Cellular Physiology, edited by Peracchia C. San Diego, CA: Academic, 1994, p. 361-377.
15. 2 in gap junction channels made of Cx50 or its D3N mutant (Abstract). Mol Biol Cell 15: 267a, 2004.
16. Peracchia C, Wang XG, Li LQ, and Peracchia LL. Inhibition of calmodulin expression prevents low-pH-induced gap junction uncoupling in Xenopus oocytes. Pflügers Arch 431: 379-387, 1996.
17. Peracchia C, Wang XG, and Peracchia LL. Is the chemical gate of connexins voltage sensitive? Behavior of Cx32 wild-type and mutant channels. Am J Physiol Cell Physiol 276: C1361-C1373, 1999.
18. Peracchia C, Wang XG, and Peracchia LL. Chemical gating of gap junction channels. Methods 20: 188-195, 2000.
19. Peracchia C, Wang XG, and Peracchia LL. Behavior of chemical- and slow voltage-sensitive gating of connexin channels: the "cork" gating hypothesis. In: Gap Junctions-Molecular Basis of Cell Communication in Health and Disease, edited by Peracchia C. San Diego, CA: Academic, 2000, p. 271-295.
20. Peracchia C, Wang XG, and Peracchia LL. Slow gating of gap junction channels and calmodulin. J Membr Biol 78: 55-70, 2000.
21. 2-sensitive? Cx45 channels and inhibition of calmodulin expression. J Membr Biol 195: 53-62, 2003.
22. 2. Cell Comm Adhes 10: 233-237, 2003.
23. Purnick PEM, Oh SH, Abrams CK, Verselis VK, and Bargiello TA. Reversal of the gating polarity of gap junctions by negative charge substitutions in the N-terminus of connexin 32. Biophys J 79: 2403-2415, 2000.
24. Purnick PEM, Benjamin DC, Verselis VK, Bargiello TA, and Dowd TL. Structure of the amino terminus of a gap junction protein. Arch Biochem Biophys 381: 181-190, 1975.
25. Rose B and Loewenstein WR. Permeability of cell junction depends on local cytoplasmic calcium activity. Nature 254: 250-252, 2000.
26. Sasaki S, Ishibashi K, Nagai T, and Marumo F. Regulation mechanisms of intracellular pH of Xenopus laevis oocyte. Biochim Biophys Acta 1137: 45-51, 1992.
27. Spray DC, Harris AL, and Bennett MVL. Equilibrium properties of a voltage-dependent junctional conductance. J Gen Physiol 77: 77-93, 1998.
28. Spray DC, Harris AL, and Bennett MV. Gap junctional conductance is a simple and sensitive function of intracellular pH. Science 211: 712-715, 1981.
29. Srivas M, Costa M, Gao Y, Fort A, Fishman GI, and Spray DC. Voltage dependence of macroscopic and unitary currents of gap junction channels formed by mouse connexin50 expressed in rat neuroblastoma cells. J Physiol 517: 673-689, 1999.
30. Stergiopoulos K, Alvarado JL, Mastroianni M, Ek-Vitorin JF, Taffet SM, and Delmar M. Hetero-domain interactions as a mechanism for the regulation of connexin channels. Circ Res 84: 1144-1155, 1999.
31. Suchyna TM, Xu LX, Gao F, Fourtner CR, and Nicholson BJ. Identification of a proline residue as a transduction element involved in voltage gating of gap junctions. Nature 365: 847-849, 1993.
32. Török K, Stauffer K, and Evans WH. Connexin 32 of gap junctions contains two cytoplasmic calmodulin-bindmg domains. Biochem J 326: 479-483, 1997.
33. Turin L and Warner AE. Carbon dioxide reversibly abolishes ionic communication between cells of early amphibian embryo. Nature 270: 56-57, 1977.
34. Verselis VK, Ginter CS, and Bargiello TA. Opposite voltage gating polarities of two closely related connexins. Nature 368: 348-351, 1994.
35. Verselis VK and Veenstra RD. Gap junction channels: permeability and voltage gating. In: Advances in Molecular and Cell Biology. Stamford, CT: JAI, 2000, vol. 30, p. 129-192.
36. Wang XG and Peracchia C. connexin 32/38 chimeras suggest a role for the second half of inner loop in gap junction gating by low pH. Am J Physiol Cell Physiol 271: C1743-C1749, 1996.
37. m-sensitive gating mechanism of gap junction channels. Pflügers Arch 435: 310-319, 1998.
38. Werner R, Levine E, Rabadan-Diehl C, and Dahl G. Gating properties of connexin32 cell-cell channels and their mutants expressed in Xenopus oocytes. Proc R Soc Lond B Biol Sci 243: 5-11, 1991.
39. White TW, Bruzzone R, Wolfram S, Paul DL, and Goodenough DA. Selective interactions among the multiple connexin proteins expressed in the vertebrate lens: the second extracellular domain is a determinant of compatibility between connexins. J Cell Biol 125: 879-892, 1994.
40. Xu X, Berthoud VM, Beyer EC, and Ebihara L. Functional role of the carboxyl terminal domain of connexin 50 in gap junctional cheannels. J Membr Biol 186: 101-112, 2002.
41. Young KC and Peracchia C. Carbon dioxide sensitive voltage gating of connexin32 and connexin32/45 chimeric channels (Abstract). Mol Biol Cell 13: 351a, 2002.
42. 2 reflects opposite voltage-gating polarity. J Membr Biol 202: 161-170, 2004.