Charges dispersed over the permeation pathway determine the charge selectivity and conductance of a Cx32 chimeric hemichannel

Journal of Physiology

Volumn 586, Issue 10, 2008, Pages 2445-2461

  Oh, Seunghoon ^a,b   Verselis, Vytas K ^a   Bargiello, Thaddeus A ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b Dankook University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTIN; CONNEXIN 32; CONNEXIN 32 CHIMERIC HEMICHANNEL; CONNEXIN 43; CONNEXIN 46; ION CHANNEL; SULFONIC ACID DERIVATIVE;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CELL MEMBRANE POTENTIAL; CELL METABOLISM; CONTROLLED STUDY; ELECTRIC POTENTIAL; FEMALE; ION CONDUCTANCE; IONIC STRENGTH; NONHUMAN; OOCYTE; PRIORITY JOURNAL; SEQUENCE ALIGNMENT; SIGNAL TRANSDUCTION; XENOPUS; CELL TRANSPORT; CONCENTRATION (PARAMETERS); INSIDE OUT PATCH CLAMP; MEMBRANE CONDUCTANCE; MEMBRANE POTENTIAL; OUTSIDE OUT PATCH CLAMP; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; CONNEXINS; ELECTRIC CONDUCTIVITY; ELECTROSTATICS; HUMANS; ION CHANNELS; MOLECULAR SEQUENCE DATA; PERMEABILITY; SIGNAL TRANSDUCTION;

EID: 43749106037     PISSN: 00223751     EISSN: 14697793     Source Type: Journal    
DOI: 10.1113/jphysiol.2008.150805     Document Type: Article

References (33)

1. Banach K, Ramanan SV & Brink PR (2000). The influence of surface charges on the conductance of the human connexin37 gap junction channel. Biophys J 78, 752-760.
2. 3. J Gen Physiol 109, 509-522.
3. Bennett MV, Zheng X & Sogin ML (1994). The connexins and their family tree. Soc Gen Physiol Series 49, 223-233.
4. Brink PR (1983). Effect of deuterium oxide on junctional membrane channel permeability. J Membr Biol 71, 79-87.
5. Bukauskas FF, Bukauskiene A & Verselis VK (2002). Conductance and permeability of the residual state of connexin43 gap junction channels. J Gen Physiol 119, 171-186.
6. Bukauskas FF, Kreuzberg MM, Rackauskas M, Bukauskiene A, Bennett MV, Verselis VK & Willecke K (2006). Properties of mouse connexin 30.2 and human connexin 31.9 hemichannels: Implications for atrioventricular conduction in the heart. Proc Natl Acad Sci U S A 103, 9726-9731.
7. Chen D & Eisenberg R (1993). Charges, currents and potentials in ionic channels of one conformation. Biophys J 64, 1405-1421.
8. Chen D, Lear J & Eisenberg B (1997). Permeation through an open channel: Poisson-Nernst-Planck theory of a synthetic ionic channel. Biophys J 72, 97-116.
9. Dong L, Liu X, Li H, Vertel BM & Ebihara L (2006). Role of the N-terminus in permeability of chicken connexin45.6 gap junctional channels. J Physiol 576, 787-799.
10. Eisenman G & Horn R (1983). Ionic selectivity revisited: The role of kinetic and equilibrium processes in ion permeation through channels. J Membr Biol 76, 197-225.
11. Gil Z, Magleby KL & Silberberg SD (1999). Membrane-pipette interactions underlie delayed voltage activation of mechanosensitive channels in Xenopus oocytes. Biophys J 76, 3118-3127.
12. Harris AL (2001). Emerging issues of connexin channels: Biophysics fills the gap. Q Rev Biophys 34, 325-472.
13. Harris AL (2007). Connexin channel permeability to cytoplasmic molecules. Prog Biophys Mol Biol 94, 120-143.
14. Hu X, Ma M & Dahl G (2006). Conductance of connexin hemichannels segregates with the first transmembrane segment. Biophys J 90, 140-150.
15. Kienker PK, DeGrado WF & Lear JD (1994). A helical-dipole model describes the single-channel current rectification of an uncharged peptide ion channel. Proc Natl Acad Sci U S A 91, 4859-4863.
16. Kienker PK & Lear JD (1995). Charge selectivity of the designed uncharged peptide ion channel Ac-(LSSLLSL) 3-CONH2. Biophys J 68, 1347-1358.
17. Kronengold J, Trexler EB, Bukauskas FF, Bargiello TA & Verselis VK (2003). Single-channel scam identifies pore-lining residues in the first extracellular loop and first transmembrane domains of cx46 hemichannels. J Gen Physiol 122, 389-405.
18. Ma M & Dahl G (2006). Cosegregation of permeability and single-channel conductance in chimeric connexins. Biophys J 90, 151-163.
19. Oh S, Abrams CK, Verselis VK & Bargiello TA (2000). Stoichiometry of transjunctional voltage-gating polarity reversal by a negative charge substitution in the amino terminus of a connexin32 chimera. J Gen Physiol 116, 13-31.
20. Oh S, Ri Y, Bennett MV, Trexler EB, Verselis VK & Bargiello TA (1997). Changes in permeability caused by connexin 32 mutations underlie X-linked Charcot-Marie-Tooth disease. Neuron 19, 927-938.
21. Oh S, Rivkin S, Tang Q, Verselis VK & Bargiello TA (2004). Determinants of gating polarity of a connexin 32 hemichannel. Biophys J 87, 912-928.
22. Oh S, Rubin JB, Bennett MV, Verselis VK & Bargiello TA (1999). Molecular determinants of electrical rectification of single channel conductance in gap junctions formed by connexins 26 and 32. J Gen Physiol 114, 339-364.
23. Purnick PE, Benjamin DC, Verselis VK, Bargiello TA & Dowd TL (2000 b). Structure of the amino terminus of a gap junction protein. Arch Biochem Biophys 381, 181-190.
24. Purnick PE, Oh S, Abrams CK, Verselis VK & Bargiello TA (2000a). Reversal of the gating polarity of gap junctions by negative charge substitutions in the N-terminus of connexin 32. Biophys J 79, 2403-2415.
25. Qu Y & Dahl G (2002). Function of the voltage gate of gap junction channels: Selective exclusion of molecules. Proc Natl Acad Sci U S A 99, 697-702.
26. Rackauskas M, Verselis VK & Bukauskas FF (2007). Permeability of homotypic and heterotypic gap junction channels formed of cardiac connexins mCx30.2, Cx40, Cx43, and Cx45. Am J Physiol Heart Circ Physiol 293, H1729-H1736.
27. Skerrett IM, Aronowitz J, Shin JH, Cymes GE, Kasperek E, Cao FL & Nicholson BJ (2002). Identification of amino acid residues lining the pore of a gap junction channel. J Cell Biol 159, 349-360.
28. Srinivas M, Calderon DP, Kronengold J & Verselis VK (2006). Regulation of connexin hemichannels by monovalent cations. J Gen Physiol 127, 67-75.
29. Trexler EB, Bukauskas FF, Kronengold J, Bargiello TA & Verselis VK (2000). The first extracellular loop is a major determinant of charge selectivity in connexin46 channels. Biophys J 79, 3036-3051.
30. Verselis VK & Brink PR (1986). The gap junction channel: Its aqueous nature as indicated by deuterium oxide effects. Biophys J 50, 1003-1007.
31. Verselis VK, Ginter CS & Bargiello TA (1994). Opposite voltage gating polarities of two closely related connexins. Nature 368, 348-351.
32. Wang HZ & Veenstra RD (1997). Monovalent ion selectivity sequences of the rat connexin43 gap junction channel. J Gen Physiol 109, 491-507.
33. Zhou XW, Pfahnl A, Werner R, Hudder A, Llanes A, Luebke A & Dahl G (1997). Identification of a pore lining segment in gap junction hemichannels. Biophys J 72, 1946-1953.