Patch oligodeoxynucleotide synthesis (POS): A novel method for synthesis of long DNA sequences and full-length genes

Biotechnology Letters

Volumn 34, Issue 4, 2012, Pages 721-728

  Yang, Guanghua ^a,c,d   Wang, Shuqi ^b   Wei, Huilin ^e   Ping, Jian ^d   Liu, Jia ^f   Xu, Lieming ^d,f   Zhang, Wenwei ^d,f  

^a Hangzhou Televector Biomedical Ltd Co   (China)

^b BRIGHAM AND WOMEN S HOSPITAL   (United States)

^c Suzhou Telebio Biotechnology Ltd Co   (China)

^d SHANGHAI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE   (China)

^e Brunswick Labs   (United States)

^f Shanghai Televector Biomedical Ltd Co   (China)

Author keywords

Catenin 1 gene; Gene synthesis; Ligation; Oligodeoxynucleotide; Polymerase chain reaction

Indexed keywords

DNA FRAGMENT; DNA MANIPULATION; DNA SYNTHESISS; DOUBLE STRANDED DNA; GENE SYNTHESIS; LIGATION; OLIGODEOXYNUCLEOTIDES; POLYADENYLATION SIGNALS; SECONDARY STRUCTURES; YELLOW FLUORESCENT PROTEINS;

BIOSYNTHESIS; DNA; GENES; OLIGONUCLEOTIDES;

POLYMERASE CHAIN REACTION;

BACTERIAL PROTEIN; BETA CATENIN; DNA; PHOTOPROTEIN; SYNTHETIC DNA; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ANIMAL; ARTICLE; BIOTECHNOLOGY; GENETICS; LIGASE CHAIN REACTION; METABOLISM; METHODOLOGY; MOLECULAR BIOLOGY; POLYMERASE CHAIN REACTION; RAT; REGULATORY RNA SEQUENCE; SIMIAN VIRUS 40;

ANIMALS; BACTERIAL PROTEINS; BETA CATENIN; BIOTECHNOLOGY; DNA; GENES, SYNTHETIC; LIGASE CHAIN REACTION; LUMINESCENT PROTEINS; MOLECULAR BIOLOGY; POLYMERASE CHAIN REACTION; RATS; RNA 3' POLYADENYLATION SIGNALS; SIMIAN VIRUS 40;

RATTUS; RATTUS NORVEGICUS; SIMIAN VIRUS 40;

EID: 84858003034     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-011-0832-0     Document Type: Article

References (21)

1. Adams SE, Johnson ID, Braddock M, Kingsman AJ, Kingsman SM, Edwards RM (1988) Synthesis of a gene for the HIV transactivator protein TAT by a novel single stranded approach involving in vivo gap repair. Nucleic Acids Res 16: 4287-4298.
2. Au LC, Yang FY, Yang WJ, Lo SH, Kao CF (1998) Gene synthesis by a LCR-based approach: high-level production of leptin-L54 using synthetic gene in Escherichia coli. Biochem Biophys Res Commun 248: 200-203.
3. Bang D, Church GM (2008) Gene synthesis by circular assembly amplification. Nat Methods 5: 37-39.
4. Chen HB, Weng JM, Jiang K, Bao JS (1990) A new method for the synthesis of a structural gene. Nucleic Acids Res 18: 871-878.
5. Ciccarelli RB, Gunyuzlu P, Huang J, Scott C, Oakes FT (1991) Construction of synthetic genes using PCR after automated DNA synthesis of their entire top and bottom strands. Nucleic Acids Res 19: 6007-6013.
6. Climie S, Santi DV (1990) Chemical synthesis of the thymidylate synthase gene. Proc Natl Acad Sci USA 87: 633-637.
7. Ecker DJ, Khan MI, Marsh J, Butt TR, Crooke ST (1987) Chemical synthesis and expression of a cassette adapted ubiquitin gene. J Biol Chem 262: 3524-3527.
8. Franklin S, Ngo B, Efuet E, Mayfield SP (2002) Development of a GFP reporter gene for Chlamydomonas reinhardtii chloroplast. Plant J 30: 733-744.
9. Gao X, Yo P, Keith A, Ragan TJ, Harris TK (2003) Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences. Nucleic Acids Res 31: e143.
10. Kosuri S, Eroshenko N, Leproust EM, Super M, Way J, Li JB et al (2010) Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips. Nat Biotechnol 28: 1295-1299.
11. Mandecki W, Bolling TJ (1988) FokI method of gene synthesis. Gene 68: 101-107.
12. Marsic D, Hughes RC, Byrne-Steele ML, Ng JD (2008) PCR-based gene synthesis to produce recombinant proteins for crystallization. BMC Biotechnol 8: 44.
13. Matzas M, Stahler PF, Kefer N, Siebelt N, Boisguerin V, Leonard JT et al (2010) High-fidelity gene synthesis by retrieval of sequence-verified DNA identified using high-throughput pyrosequencing. Nat Biotechnol 28: 1291-1294.
14. Mehrnejad F, Naderi-Manesh H, Ranjbar B, Maroufi B, Asoodeh A, Doustdar F (2008) PCR-based gene synthesis, molecular cloning, high level expression, purification, and characterization of novel antimicrobial peptide, Brevinin-2R, in Escherichia Coli. Appl Biochem Biotechnol 149: 109-118.
15. Smith HO, Hutchison CA III, Pfannkoch C, Venter JC (2003) Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotides. Proc Natl Acad Sci USA 100: 15440-15445.
16. Sproat BS, Gait MJ (1985) Chemical synthesis of a gene for somatomedin C. Nucleic Acids Res 13: 2959-2977.
17. Stemmer WP, Crameri A, Ha KD, Brennan TM, Heyneker HL (1995) Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides. Gene 164: 49-53.
18. Strizhov N, Keller M, Mathur J, Koncz-Kalman Z, Bosch D, Prudovsky E et al (1996) A synthetic cryIC gene, encoding a Bacillus thuringiensis delta-endotoxin, confers Spodoptera resistance in alfalfa and tobacco. Proc Natl Acad Sci USA 93: 15012-15017.
19. Wu G, Wolf JB, Ibrahim AF, Vadasz S, Gunasinghe M, Freeland SJ (2006) Simplified gene synthesis: a one-step approach to PCR-based gene construction. J Biotechnol 124: 496-503.
20. Xiong AS, Yao QH, Peng RH, Li X, Fan HQ, Cheng ZM et al (2004) A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences. Nucleic Acids Res 32: e98.
21. Xiong AS, Peng RH, Zhuang J, Gao F, Li Y, Cheng ZM et al (2008) Chemical gene synthesis: strategies, softwares, error corrections, and applications. FEMS Microbiol Rev 32: 522-540.