PCR-based gene synthesis, molecular cloning, high level expression, purification, and characterization of novel antimicrobial peptide, Brevinin-2R, in Escherichia coli

Applied Biochemistry and Biotechnology

Volumn 149, Issue 2, 2008, Pages 109-118

  Mehrnejad, Faramarz ^a   Naderi Manesh, Hossein ^a   Ranjbar, Bijan ^a   Maroufi, Bahman ^a   Asoodeh, Ahmad ^b   Doustdar, Farahnoosh ^c  

^a TARBIAT MODARES UNIVERSITY   (Iran)

^b FERDOWSI UNIVERSITY OF MASHHAD   (Iran)

^c AHVAZ JUNDISHAPUR UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

Antimicrobial peptide; Brevinin 2R; Expression; Fusion partner; PCR based gene synthesis; Rana ridibunda

Indexed keywords

ANTIMICROBIAL PEPTIDE; BREVININ-2R; EXPRESSION; FUSION PARTNER; PCR-BASED GENE SYNTHESIS; RANA RIDIBUNDA;

CHARACTERIZATION; CHROMATOGRAPHIC ANALYSIS; CLONING; ESCHERICHIA COLI; POLYPEPTIDES; PROTEINS; PURIFICATION;

AMINES;

BLOOD CLOTTING FACTOR 10A; BREVININ 2R; HYBRID PROTEIN; POLYPEPTIDE ANTIBIOTIC AGENT; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; AMPHIBIAN PROTEIN; ANTIINFECTIVE AGENT; ANTIMICROBIAL CATIONIC PEPTIDE; BREVININ 2, RANA; BREVININ-2, RANA; PEPTIDE;

ANTIMICROBIAL ACTIVITY; ARTICLE; BACTERIAL GENE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; DRUG DETERMINATION; DRUG ISOLATION; DRUG MANUFACTURE; DRUG PURIFICATION; DRUG SYNTHESIS; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE EXPRESSION; GENE ISOLATION; GENE OVEREXPRESSION; GENE SYNTHESIS; GENE VECTOR; KLEBSIELLA PNEUMONIAE; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; AMINO ACID SEQUENCE; CHEMISTRY; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS;

ESCHERICHIA COLI; FUNGI; RANA RIDIBUNDA;

AMINO ACID SEQUENCE; AMPHIBIAN PROTEINS; ANTI-INFECTIVE AGENTS; ANTIMICROBIAL CATIONIC PEPTIDES; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; MOLECULAR SEQUENCE DATA; PEPTIDES; POLYMERASE CHAIN REACTION;

EID: 50049112246     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-007-8024-z     Document Type: Article

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