Improved detection of Rhodococcus coprophilus with a new quantitative PCR assay

Applied Microbiology and Biotechnology

Volumn 93, Issue 5, 2012, Pages 2161-2169

  Wicki, Melanie ^a,b,c   Auckenthaler, Adrian ^d   Felleisen, Richard ^a   Liniger, Marianne ^a   Loutre, Caroline ^e   Niederhauser, Isabel ^a   Tanner, Marcel ^b,c   Baumgartner, Andreas ^a  

^a FEDERAL OFFICE OF PUBLIC HEALTH   (Switzerland)

^b SWISS TROPICAL AND PUBLIC HEALTH INSTITUTE   (Switzerland)

^c UNIVERSITY OF BASEL   (Switzerland)

^d Office for Environmental Protection and Energy   (Switzerland)

^e UNIVERSITY OF LAUSANNE   (Switzerland)

Author keywords

Contamination; LightCycler; Sewage; Validation; Water quality

Indexed keywords

16S RRNA GENE; AGRICULTURAL PRACTICES; DETECTION LIMITS; DETECTION METHODS; FAECAL CONTAMINATION; FIELD INVESTIGATION; LIGHTCYCLER; LIQUID MANURE; MICROBIAL SOURCE TRACKING; MOLECULAR APPROACH; PCR ASSAY; QUANTITATIVE PCR; QUANTITATIVE POLYMERASE CHAIN REACTION; RHODOCOCCUS; SPRING WATER; SWITZERLAND; VALIDATION;

AGRICULTURE; ANIMALS; ASSAYS; CONTAMINATION; FILTRATION; GENES; GROUNDWATER; MANURES; RNA; SEWAGE; WATER QUALITY; WATER RESOURCES;

POLYMERASE CHAIN REACTION;

RNA 16S;

AGRICULTURAL PRACTICE; BACTERIUM; DETECTION METHOD; FIELD METHOD; FILTRATION; MANURE; MOLECULAR ANALYSIS; POLYMERASE CHAIN REACTION; SEWAGE; SPRING WATER; SURFACE WATER; TRACKING; WATER POLLUTION;

ARTICLE; BACTERIUM COLONY; BACTERIUM DETECTION; CONTROLLED STUDY; HOST; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; RHODOCOCCUS; RHODOCOCCUS COPROPHILUS; SENSITIVITY AND SPECIFICITY; SWITZERLAND; WATER CONTAMINATION;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; DNA, BACTERIAL; DNA, RIBOSOMAL; REAL-TIME POLYMERASE CHAIN REACTION; RHODOCOCCUS; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; SWITZERLAND; WATER MICROBIOLOGY; WATER POLLUTION;

SWITZERLAND;

ANIMALIA; RHODOCOCCUS COPROPHILUS;

EID: 84857917784     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-3888-4     Document Type: Article

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