Application of polymerase chain reaction (PCR) and TaqMan™ PCR techniques to the detection and identification of Rhodococcus coprophilus in faecal samples

Journal of Microbiological Methods

Volumn 47, Issue 3, 2001, Pages 355-368

  Savill, Marion G ^a   Murray, Sonya R ^a   Scholes, Paula ^a   Maas, Els W ^a   McCormick, Rachel E ^a   Moore, Edward B ^b   Gilpin, Brent J ^a  

^a ESR Christchurch Science Centre   (New Zealand)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

Faecal source identification; Rhodococcus coprophilus; TaqMan

Indexed keywords

BACTERIAL DNA; RNA 16S;

ANIMAL CELL; ARTICLE; BACTERIUM; CONTROLLED STUDY; COW; DEER; DUCK; FECES ANALYSIS; GENE AMPLIFICATION; HORSE; HUMAN; INTERMETHOD COMPARISON; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RABBIT; RHODOCOCCUS; RNA GENE; SHEEP; SPECIES DIFFERENCE; SWINE; WATER POLLUTION;

ANIMALS; BACTERIAL TYPING TECHNIQUES; CATTLE; DEER; DNA, BACTERIAL; DNA, RIBOSOMAL; DUCKS; FECES; HORSES; HUMANS; OPOSSUMS; POLYMERASE CHAIN REACTION; RHODOCOCCUS; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; SHEEP; SWINE; TAQ POLYMERASE;

ANAS SP.; ANIMALIA; BACTERIA (MICROORGANISMS); CERVIDAE; COPROPHILUS; EQUUS CABALLUS; ORYCTOLAGUS CUNICULUS; OVIS ARIES; RHODOCOCCUS COPROPHILUS; SUS SCROFA;

EID: 0035170553     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(01)00343-8     Document Type: Article

References (45)

1. Ecological studies on nocardioforms and other actinomycetes in aquatic habitats
2. An assessment of Bacteroides fragilis group of organisms as indicators of human faecal pollution
3. The genus Rhodococcus
4. Identification and environmental detection of Rhodococcus species by 16S rDNA-targeted PCR
5. Rapid and simple method for the purification of nucleic acids
6. An inexpensive and simple method for DNA purifications on silica particles
7. The evaluation of a fluorogenic polymerase chain reaction assay for the detection of Salmonella species in food commodities
8. Detection of Salmonella enteritidis in faeces from poultry using booster polymerase chain reaction and oligonucleotide primers specific for all members of the genus Salmonella
9. Survival and detection of Bacteroides spp., prospective indicator bacteria
10. Phenotypic differentiation of bifidobacteria of human and animal origins
11. Evaluation of indicators of indicators for assessment of human and animal faecal pollution of surface run-off
12. Evaluation of Bacteroides fragilis bacteriophages as indicators of the virological quality of water
13. Two independently regulated cytochromes P-450 in a Rhodococcus rhodochrous strain that degrades 2-ethoxyphenol and 4-methoxybenzoate
14. Design and evaluation of Bacteroides DNA probes for the specific detection of human faecal pollution
15. Allelic discrimination by nick-translation PCR with fluorogenic probes
16. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridisation
17. The RDP (Ribosomal Database Project)
18. Occurrence of Rhodococcus coprophilus and associated actinomycetes in faeces, sewage, and freshwater
19. Sorbitol fermenting bifidobacteria as specific indicators of human faecal pollution
20. Bacteriological methods of distinguishing between human and animal faecal pollution of water: Results of fieldwork in Nigeria and Zimbabwe
21. A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species
22. Rapid isolation of high molecular weight plant material
23. Application of the 5′-nuclease PCR assay in evaluation and development of methods for quantitative detection of Campylobacter jejuni
24. Detection of viable Listeria monocytogenes with a 5′ nuclease PCR assay
25. A selective medium for the enumeration of Streptococcus bovis by membrane filtration
26. Investigation of the survival characteristics of Rhodococcus coprophilus and certain faecal indicator bacteria
27. Development of a quantitative real-time detection assay for hepatitis B virus DNA and comparison with two commercial assays
28. Improved tools for Biological Sequence Analysis
29. Phylogenetic analysis of the genera Rhodococcus and Nocardia and evidence for the evolutionary origin of the genus Nocardia from within the radiation of Rhodococcus species
30. Sorbitol-fermenting bifidobacteria as indicators of diffuse human faecal pollution in estuarine watersheds
31. Ecology of Rhodococcus coprophilus and associated actinomycetes in fresh water and agricultural habitats
32. Rapid identification of Yersinia enterocolitica in blood by the 5′ nuclease PCR assay
33. Isolation and identification of actinomycetes present in activated sludge scum
34. Distinguishing human from animal faecal contamination in water: A review
35. Identification and characterization of ecologically significant prokaryotes in the sediment of freshwater lakes: Molecular and cultivation studies
36. Protection of DNA from high LET radiation by two OH radical scavengers, tris (hydroxymethyl) aminomethane and 2-mercaptoethanol
37. Bacteriophages active against Bacteroides fragilis in sewage-polluted waters
38. Pseudomonas aeruginosa and Escherichia coli in sewage and fresh water
39. Development of molecular methods for identification of Streptococcus bovis from human and ruminal origins
40. Miniprep of bacterial genomic DNA
41. Bacterial evolution
42. Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids