Interplay of a ligand sensor and an enzyme in controlling expression of the Saccharomyces cerevisiae GAL genes

Eukaryotic Cell

Volumn 11, Issue 3, 2012, Pages 334-342

  Abramczyk, Dariusz ^a   Holden, Stacey ^a   Page, Christopher J ^a   Reece, Richard J ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; GALACTOKINASE; GALACTOSE; PHOTOPROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN; TRANSCRIPTION FACTOR; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ARTICLE; CELL NUCLEUS; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; PROTEIN BINDING; REPORTER GENE; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION; ULTRASTRUCTURE;

BACTERIAL PROTEINS; CELL NUCLEUS; GALACTOKINASE; GALACTOSE; GENE EXPRESSION REGULATION, FUNGAL; GENES, REPORTER; LUMINESCENT PROTEINS; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SIGNAL TRANSDUCTION; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 84857709060     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.05294-11     Document Type: Article

References (32)

1. Barberis A, et al. 1995. Contact with a component of the polymerase II holoenzyme suffices for gene activation. Cell 81:359-368.
2. Bhat PJ, Hopper JE. 1991. The mechanism of inducer formation in gal3 mutants of the yeast galactose system is independent of normal galactose metabolism and mitochondrial respiratory function. Genetics 128:233-239.
3. Bhat PJ, Hopper JE. 1992. Overproduction of the GAL1 or GAL3 protein causes galactose-independent activation of the GAL4 protein: evidence for a new model of induction for the yeast GAL/MEL regulon. Mol. Cell. Biol. 12:2701-2707.
4. Bhaumik SR, Raha T, Aiello DP, Green MR. 2004. In vivo target of a transcriptional activator revealed by fluorescence resonance energy transfer. Genes Dev. 18:333-343.
5. Egriboz O, Jiang F, Hopper JE. 2011. Rapid GAL gene switch of Saccharomyces cerevisiae depends on nuclear Gal3, not nucleocytoplasmic trafficking of Gal3 and Gal80. Genetics 189:825-836.
6. Gietz RD, Sugino A. 1988. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74:527-534.
7. Gietz RD, Woods RA. 2006. Yeast transformation by the LiAc/SS carrier DNA/PEG method. Methods Mol. Biol. 313:107-120.
8. Giniger E, Varnum SM, Ptashne M. 1985. Specific DNA binding of GAL4, a positive regulatory protein of yeast. Cell 40:767-774.
9. Griggs DW, Johnston M. 1991. Regulated expression of the GAL4 activator gene in yeast provides a sensitive genetic switch for glucose repression. Proc. Natl. Acad. Sci. U. S. A. 88:8597-8601.
10. Jiang F, Frey BR, Evans ML, Friel JC, Hopper JE. 2009. Gene activation by dissociation of an inhibitor from a transcriptional activation domain. Mol. Cell. Biol. 29:5604-5610.
11. Kumar PR, Yu Y, Sternglanz R, Johnston SA, Joshua-Tor L. 2008. NADP regulates the yeast GAL induction system. Science 319:1090-1092.
12. Leuther KK, Johnston SA. 1992. Nondissociation of GAL4 and GAL80 in vivo after galactose induction. Science 256:1333-1335.
13. Ma J, Ptashne M. 1987. Deletion analysis of GAL4 defines two transcriptional activating segments. Cell 48:847-853.
14. Nguyen AW, Daugherty PS. 2005. Evolutionary optimization of fluorescent proteins for intracellular FRET. Nat. Biotechnol. 23:355-360.
15. Ohashi Y, Brickman JM, Furman E, Middleton B, Carey M. 1994. Modulating the potency of an activator in a yeast in vitro transcription system. Mol. Cell. Biol. 13:2731-2739.
16. Panse VG, Kuster B, Gerstberger T, Hurt E. 2003. Unconventional tethering of Ulp1 to the transport channel of the nuclear pore complex by karyopherins. Nat. Cell Biol. 5:21-27.
17. Parthun MR, Jaehning JA. 1992. A transcriptionally active form of GAL4 is phosphorylated and associated with GAL80. Mol. Cell. Biol. 12:4981-4987.
18. Peng G, Hopper JE. 2000. Evidence for Gal3p's cytoplasmic location and Gal80p's dual cytoplasmic-nuclear location implicates new mechanisms for controlling Gal4p activity in Saccharomyces cerevisiae. Mol. Cell. Biol. 20:5140-5148.
19. Peng G, Hopper JE. 2002. Gene activation by interaction of an inhibitor with a cytoplasmic signaling protein. Proc. Natl. Acad. Sci. U. S. A. 99: 8548-8553.
20. Platt A, Reece RJ. 1998. The yeast galactose genetic switch is mediated by the formation of a Gal4p/Gal80p/Gal3p complex. EMBO J. 17:4086-4091.
21. Platt A, Ross HC, Hankin S, Reece RJ. 2000. The insertion of two amino acids into a transcriptional inducer converts it into a galactokinase. Proc. Natl. Acad. Sci. U. S. A. 97:3154-3159.
22. Rudolph H, Koenig-Rauseo I, Hinnen A. 1985. One-step gene replacement in yeast by cotransformation. Gene 36:87-95.
23. Sellick CA, Reece RJ. 2005. Eukaryotic transcription factors as direct nutrient sensors. Trends Biochem. Sci. 30:405-412.
24. Sil AK, et al. 1999. The Gal3p-Gal80p-Gal4p transcription switch of yeast: Gal3p destabilizes the Gal80p-Gal4p complex in response to galactose and ATP. Mol. Cell. Biol. 19:7828-7840.
25. Thoden JB, Ryan LA, Reece RJ, Holden HM. 2008. The interaction between an acidic transcriptional activator and its inhibitor: the molecular basis of Gal4p recognition by Gal80p. J. Biol. Chem. 283:30266-30272.
26. Thoden JB, Sellick CA, Reece RJ, Holden HM. 2007. Understanding a transcriptional paradigm at the molecular level: the structure of yeast Gal80p. J. Biol. Chem. 282:1534-1538.
27. Thoden JB, Sellick CA, Timson DJ, Reece RJ, Holden HM. 2005. Molecular structure of Saccharomyces cerevisiae Gal1p-a bifunctional galactokinase and transcriptional inducer. J. Biol. Chem. 280:36905-36911.
28. Wightman R, Bell R, Reece RJ. 2008. Localization and interaction of the proteins constituting the GAL genetic switch in Saccharomyces cerevisiae. Eukaryot. Cell 7:2061-2068.
29. Winston F, Dollard C, Ricupero-Hovasse SL. 1995. Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C. Yeast 11:53-55.
30. Wu Y, Reece RJ, Ptashne M. 1996. Quantitation of putative activatortarget affinities predicts transcriptional activating potentials. EMBO J. 15: 3951-3963.
31. Yarger JG, Halvorson HO, Hopper JE. 1984. Regulation of galactokinase (GAL1) enzyme accumulation in Saccharomyces cerevisiae. Mol. Cell. Biochem. 61:173-182.
32. Zenke FT, et al. 1996. Activation of Gal4p by galactose-dependent interaction of galactokinase and Gal80p. Science 272:1662-1665.