Construction of membrane-anchoring fusion protein of Thermococcus kodakaraensis glycerol kinase and its application to repetitive batchwise reactions

Journal of Bioscience and Bioengineering

Volumn 113, Issue 4, 2012, Pages 521-525

  Restiawaty, Elvi ^a   Honda, Kohsuke ^a,b   Okano, Kenji ^a   Hirota, Ryuichi ^c   Omasa, Takeshi ^a,d   Kuroda, Akio ^c   Ohtake, Hisao ^a  

^a OSAKA UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c HIROSHIMA UNIVERSITY   (Japan)

^d UNIVERSITY OF TOKUSHIMA   (Japan)

Author keywords

Glycerol kinase; Polyphosphate kinase; Thermophilic enzyme; Whole cell catalyst; YedZ

Indexed keywords

BATCHWISE REACTIONS; E. COLI; FUSION PROTEINS; GLYCEROL KINASE; HYPERTHERMOPHILES; INHIBITORY EFFECT; MEMBRANE FRACTION; POLYPHOSPHATES; REACTION MIXTURE; RECOMBINANT CELLS; SPACE BETWEEN; STOICHIOMETRIC CONVERSION; THERMOCOCCUS KODAKARAENSIS; THERMOPHILIC ENZYME; THERMUS THERMOPHILUS; YEDZ;

CELLS; CENTRIFUGATION; CYTOLOGY; DEHALOGENATION; ENZYMES; ESCHERICHIA COLI; MAGNESIUM; METAL IONS; RECOMBINANT PROTEINS;

GLYCEROL;

CHELATING AGENT; GLYCEROL KINASE; GLYCEROPHOSPHATE; MEMBRANE FUSION PROTEIN; MEMBRANE PROTEIN; METAL ION; POLYPHOSPHATE; POLYPHOSPHATE KINASE; UNCLASSIFIED DRUG; YEDZ PROTEIN;

ARTICLE; BACTERIAL CELL; CENTRIFUGATION; CROSS COUPLING REACTION; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME INHIBITION; ESCHERICHIA COLI; HEAT; INCUBATION TEMPERATURE; INCUBATION TIME; NONHUMAN; THERMOCOCCUS KODAKARENSIS; THERMUS THERMOPHILUS;

ESCHERICHIA COLI; GLYCEROL; GLYCEROL KINASE; HOT TEMPERATURE; INDUSTRIAL MICROBIOLOGY; MEMBRANE FUSION PROTEINS; POLYPHOSPHATES; THERMOCOCCUS; TIME FACTORS;

ESCHERICHIA COLI; THERMOCOCCUS KODAKARENSIS; THERMUS THERMOPHILUS;

EID: 84857686753     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.11.016     Document Type: Article

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