Excitation spectra and brightness optimization of two-photon excited probes

Biophysical Journal

Volumn 102, Issue 4, 2012, Pages 934-944

  Mütze, Jörg ^a,b   Iyer, Vijay ^a   MacKlin, John J ^a   Colonell, Jennifer ^a   Karsh, Bill ^a   Petrášek, Zdeněk ^b   Schwille, Petra ^b   Looger, Loren L ^a   Lavis, Luke D ^a   Harris, Timothy D ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; FLUORESCENT DYE; RHODAMINE;

ABSORPTION; ARTICLE; CHEMISTRY; METHODOLOGY; PHOTON; SPECTROFLUOROMETRY;

ABSORPTION; CALCIUM; FLUORESCENT DYES; PHOTONS; RHODAMINES; SPECTROMETRY, FLUORESCENCE;

EID: 84857241902     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2011.12.056     Document Type: Article

References (47)

1. W.R. Zipfel, R.M. Williams, and W.W. Webb Nonlinear magic: multiphoton microscopy in the biosciences Nat. Biotechnol. 21 2003 1369 1377
2. W. Denk, J.H. Strickler, and W.W. Webb Two-photon laser scanning fluorescence microscopy Science 248 1990 73 76
3. M.A. Albota, C. Xu, and W.W. Webb Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm Appl. Opt. 37 1998 7352 7356
4. M. Kauert, and P.C. Stoller J. Ricka Absolute measurement of molecular two-photon absorption cross-sections using a fluorescence saturation technique Opt. Express 14 2006 8434 8447
5. D.A. Oulianov, and I.V. Tomov P.M. Rentzepis Observations on the measurement of two-photon absorption cross-section Opt. Commun. 191 2001 235 243
6. P. Tian, and W.S. Warren Ultrafast measurement of two-photon absorption by loss modulation Opt. Lett. 27 2002 1634 1636
7. P. Sengupta, and J. Balaji S. Maiti Sensitive measurement of absolute two-photon absorption cross sections J. Chem. Phys. 112 2000 9201 9205
8. R. Kapoor, C.S. Friend, and A. Patra Two-photon-excited absolute emission cross-sectional measurements calibrated with a luminance meter J. Opt. Soc. Am. B 20 2003 1550 1554
9. M. Sheik-Bahae, and A.A. Said E.W. Van Stryland Sensitive measurement of optical nonlinearities using a single beam IEEE J. Quantum Electron. 26 1990 760 769
10. J.P. Hermann, and J. Ducuing Absolute measurement of two-photon cross section Phys. Rev. A 5 1972 2557 2568
11. C. Xu, and W. Zipfel W.W. Webb Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy Proc. Natl. Acad. Sci. USA 93 1996 10763 10768
12. C. Xu, and W.W. Webb Measurement of two-photon excitation cross sections of molecular fluorophores with data from 690 to 1050 nm J. Opt. Soc. Am. B 13 1996 481 491
13. C. Xu, and R.M. Williams W.W. Webb Multiphoton excitation cross-sections of molecular fluorophores Bioimaging 4 1996 198 207
14. N.S. Makarov, M. Drobizhev, and A. Rebane Two-photon absorption standards in the 550-1600 nm excitation wavelength range Opt. Express 16 2008 4029 4047
15. D.R. Larson, and W.R. Zipfel W.W. Webb Water-soluble quantum dots for multiphoton fluorescence imaging in vivo Science 300 2003 1434 1436
16. V. Iyer, M.J. Rossow, and N.M. Waxham Peak two-photon molecular brightness of fluorophores is a robust measure of quantum efficiency and photostability J. Opt. Soc. Am. B 23 2006 1420 1433
17. P. Schwille, and U. Haupts W.W. Webb Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation Biophys. J. 77 1999 2251 2265
18. Z. Petrásek, and P. Schwille Photobleaching in two-photon scanning fluorescence correlation spectroscopy Chem. Phys. Chem 9 2008 147 158
19. A. Nagy, J. Wu, and K.M. Berland Observation volumes and γ-factors in two-photon fluorescence fluctuation spectroscopy Biophys. J. 89 2005 2077 2090
20. K.G. Heinze, A. Koltermann, and P. Schwille Simultaneous two-photon excitation of distinct labels for dual-color fluorescence crosscorrelation analysis Proc. Natl. Acad. Sci. USA 97 2000 10377 10382
21. J. Mütze, T. Ohrt, and P. Schwille Fluorescence correlation spectroscopy in vivo Laser Photonics Rev. 5 2011 52 67
22. D.E. Koppel Statistical accuracy in fluorescence correlation spectroscopy Phys. Rev. A 10 1974 1938 1945
23. N. Ji, J.C. Magee, and E. Betzig High-speed, low-photodamage nonlinear imaging using passive pulse splitters Nat. Methods 5 2008 197 202
24. Z. Petrásek, and P. Schwille Precise measurement of diffusion coefficients using scanning fluorescence correlation spectroscopy Biophys. J. 94 2008 1437 1448
25. W. Demtröder Laser Spectroscopy. Vol. 1: Basic Principles 2008 Springer New York
26. J. Mütze, Z. Petrásek, and P. Schwille Independence of maximum single molecule fluorescence count rate on the temporal and spectral laser pulse width in two-photon FCS J. Fluoresc. 17 2007 805 810
27. P.S. Dittrich, and P. Schwille Photobleaching and stabilization of fluorophores used for single-molecule analysis with one- and two-photon excitation Appl. Phys. B 73 2001 829 837
28. D. Magde, E.L. Elson, and W.W. Webb Fluorescence correlation spectroscopy. II. An experimental realization Biopolymers 13 1974 29 61
29. C. Xu, and W.W. Webb Topics in Fluorescence Spectroscopy. Vol. 5: Nonlinear and Two-Photon-Induced Fluorescence 1997 Plenum Press New York
30. J. Mertz Molecular photodynamics involved in multi-photon excitation fluorescence microscopy Eur. Phys. J. D. 3 1998 53 66
31. N. Panchuk-Voloshina, and R.P. Haugland R.P. Haugland Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates J. Histochem. Cytochem. 47 1999 1179 1188
32. F. Helmchen, and W. Denk Deep tissue two-photon microscopy Nat. Methods 2 2005 932 940
33. Z.F. Mainen, and M. Maletic-Savatic K. Svoboda Two-photon imaging in living brain slices Methods 18 1999 231 239
34. K. Svoboda, D.W. Tank, and W. Denk Direct measurement of coupling between dendritic spines and shafts Science 272 1996 716 719
35. R.M. Paredes, and J.C. Etzler J.D. Lechleiter Chemical calcium indicators Methods 46 2008 143 151
36. M. Mank, and O. Griesbeck Genetically encoded calcium indicators Chem. Rev. 108 2008 1550 1564
37. 2+ indicator GCaMP2 Proc. Natl. Acad. Sci. USA 103 2006 4753 4758
38. L. Tian, and S.A. Hires L.L. Looger Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators Nat. Methods 6 2009 875 881
39. S.J. Wawilow The fluorescence yield of dye solutions as a function of the exciting light wavelength II. Z. Phys. 42 1927 311 318
40. A. Hopt, and E. Neher Highly nonlinear photodamage in two-photon fluorescence microscopy Biophys. J. 80 2001 2029 2036
41. G.H. Patterson, and D.W. Piston Photobleaching in two-photon excitation microscopy Biophys. J. 78 2000 2159 2162
42. R. Niesner, W. Roth, and K.-H. Gericke Photophysical aspects of single-molecule detection by two-photon excitation with consideration of sequential pulsed illumination Chem. Phys. Chem 5 2004 678 687
43. T.S. Chen, and S.Q. Zeng W. Zhou High-order photobleaching of green fluorescent protein inside live cells in two-photon excitation microscopy Biochem. Biophys. Res. Commun. 291 2002 1272 1275
44. E.J. Sanchez, and L. Novotny X.S. Xie Room-temperature fluorescence imaging and spectroscopy of single molecules by two-photon excitation J. Phys. Chem. A 101 1997 7019 7023
45. G. Donnert, C. Eggeling, and S.W. Hell Major signal increase in fluorescence microscopy through dark-state relaxation Nat. Methods 4 2007 81 86
46. J. Widengren, and A. Chmyrov C.A. Seidel Strategies to improve photostabilities in ultrasensitive fluorescence spectroscopy J. Phys. Chem. A 111 2007 429 440
47. T.J. Mitchison, and K.E. Sawin A. Mallavarapu Caged fluorescent probes Methods Enzymol. 291 1998 63 78