Rapid filtration separation-based sample preparation method for Bacillus spores in powdery and environmental matrices

Applied and Environmental Microbiology

Volumn 78, Issue 5, 2012, Pages 1505-1512

  Isabel, Sandra ^a   Boissinot, Maurice ^a   Charlebois, Isabelle ^a   Fauvel, Chantal M ^a   Shi, Lu E ^a   Lévesque, Julie Christine ^b   Paquin, Amélie T ^a   Bastien, Martine ^a   Stewart, Gale ^a   Leblanc, Éric ^a   Sato, Sachiko ^b   Bergeron, Michel G ^a  

^a LAVAL UNIVERSITY   (Canada)

^b University Laval   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACILLUS ANTHRACIS; BACILLUS ATROPHAEUS; BACILLUS SPORE; BACTERIAL LYSIS; BACTERIAL SPORE; BIOTHREAT AGENTS; BIOWARFARES; DNA EXTRACTION; ENVIRONMENTAL MATRIXES; ENVIRONMENTAL SAFETY; ENVIRONMENTAL SAMPLE; FIELD APPLICATION; LABORATORY FACILITIES; NUCLEIC ACID DETECTION; PCR DETECTION; PCR-BASED DETECTION; QUALITY LEVELS; SAMPLE PREPARATION METHODS; SAMPLE PREPARATION PROCEDURE; TECHNICAL CHALLENGES; VERSATILE METHODS;

BACILLI; BACTERIOLOGY; POLYMERASE CHAIN REACTION; SEPARATION;

ENVIRONMENTAL TECHNOLOGY;

BACTERIAL DNA;

AUTOMATION; BACTERIUM; BIOLOGICAL WEAPON; ENVIRONMENTAL PROTECTION; FILTRATION; POLYMERASE CHAIN REACTION; SEPARATION; SPORE; TECHNOLOGICAL DEVELOPMENT;

ARTICLE; BACILLUS; BACTERIAL SPORE; EVALUATION; FILTRATION; GENETICS; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; TIME;

BACILLUS; BACTERIOLOGICAL TECHNIQUES; DNA, BACTERIAL; ENVIRONMENTAL MICROBIOLOGY; FILTRATION; SPECIMEN HANDLING; SPORES, BACTERIAL; TIME FACTORS;

BACILLUS (BACTERIUM); BACILLUS ANTHRACIS; BACILLUS ATROPHAEUS; BACTERIA (MICROORGANISMS);

EID: 84857088923     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.06696-11     Document Type: Article

References (17)

1. Carrera M, Zandomeni RO, Fitzgibbon J, Sagripanti JL. 2007. Difference between the spore sizes of Bacillus anthracis and other Bacillus species. J. Appl. Microbiol. 102:303-312.
2. Dauphin LA, Moser BD, Bowen MD. 2009. Evaluation of five commercial nucleic acid extraction kits for their ability to inactivate Bacillus anthracis spores and comparison of DNA yields from spores and spiked environmental samples. J. Microbiol. Methods 76:30-37.
3. Geissler M, et al. 2011. Extraction of nucleic acids from bacterial spores using bead-based mechanical lysis on a plastic chip. Eng. Life Sci. 11:174-181.
4. Gilchrist MJR, McKinney WP, Miller JM, Weissfield AS. 2000. Cumitech 33, Laboratory safety, management, and diagnosis of biological agents associated with bioterrorism. Coordinating ed, Snyder JW. ASM Press, Washington, DC.
5. Kellogg DE, et al. 1994. TaqStart antibody: "hot start" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. Biotechniques 16:1134-1137.
6. Kowalski WJ, Bahnfleth W. July 1998. Airborne respiratory diseases and mechanical systems for control of microbes. HPAC Eng. 70(7):34-48.
7. Laflamme C, Lavigne S, Ho J, Duchaine C. 2004. Assessment of bacterial endospore viability with fluorescent dyes. J. Appl. Microbiol. 96:684-692.
8. LeClaire RD, Pitt MLM. 2005. Biological weapons defense: effect levels, p 41-64. In Lindler LE, Lebada FJ, Korch GW (ed), Biological weapons defense: infectious diseases and counterbioterrorism. Humana Press, Totowa, NJ.
9. Lim DV, Simpson JM, Kearns EA, Kramer MF. 2005. Current and developing technologies for monitoring agents of bioterrorism and biowarfare. Clin. Microbiol. Rev. 18:583-607.
10. Luna VA, et al. 2003. Novel sample preparation method for safe and rapid detection of Bacillus anthracis spores in environmental powders and nasal swabs. J. Clin. Microbiol. 41:1252-1255.
11. Picard FJ, et al. 2009. Internal control for nucleic acid testing based on the use of purified Bacillus atrophaeus subsp. globigii spores. J. Clin. Microbiol. 47:751-757.
12. Rose HL, et al. 2011. Comparison of eight methods for the extraction of Bacillus atrophaeus spore DNA from eleven common interferents and a common swab. PLoS One 6:e22668.
13. Rotz LD, Khan AS, Lillibridge SR, Ostroff SM, Hughes JM. 2002. Public health assessment of potential biological terrorism agents. Emerg. Infect. Dis. 8:225-230.
14. Shoham DJ, Jacobsen SM. 2007. Technical intelligence in retrospect: the 2001 anthrax letter powder. Int. J. Intelligence Counter Intelligence 20: 79-105.
15. U.S. Department of Justice. 19 February 2010. Amerithrax investigative summary. U.S. Department of Justice, Washington, DC. http://www.justice.gov/amerithrax/docs/amx-investigative-summary.pdf.
16. Wills B, Leikin J, Rhee J, Saeedi B. 2008. Analysis of suspicious powders following the post 9/11 anthrax scare. J. Med. Toxicol. 4:93-95.
17. Wolffs PF, Glencross K, Thibaudeau R, Griffiths MW. 2006. Direct quantitation and detection of salmonellae in biological samples without enrichment, using two-step filtration and real-time PCR. Appl. Environ. Microbiol. 72:3896-3900.