Assessment of bacterial endospore viability with fluorescent dyes

Journal of Applied Microbiology

Volumn 96, Issue 4, 2004, Pages 684-692

  Laflamme, C ^a   Lavigne, S ^a   Ho, J ^b   Duchaine, C ^a,c  

^a LAVAL UNIVERSITY   (Canada)

^b CFB Suffield   (Canada)

^c UNIVERSITÉ LAVAL   (Canada)

Author keywords

Bacillus spores; Flow cytometry; Fluorescence microscopy; Viability assays

Indexed keywords

BACILLUS CEREUS; BACTERIOLOGY; CHLORINE COMPOUNDS; FLUORESCENCE; IRRADIATION; TEMPERATURE CONTROL;

B.SUBTILIS; BACILLUS ENDOSPORES; BACILLUS SPORE; BACTERIAL ENDOSPORES; ENERGIZATION; FLUORESCENT DYES; PERMEABILIZATION; RESPIRATORY ACTIVITY; VEGETATIVE CELLS; VIABILITY ASSAY;

FLUORESCENCE MICROSCOPY;

5 CYANO 2,3 DIOTOLYLTETRAZOLIUM CHLORIDE; FLUORESCENT DYE; UNCLASSIFIED DRUG;

MICROBIAL ACTIVITY;

ARTICLE; BACILLUS CEREUS; BACILLUS COAGULANS; BACILLUS SUBTILIS; BACTERIAL ENDOSPORE; BACTERIUM CULTURE; CELL VIABILITY; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; GAMMA IRRADIATION; MEMBRANE PERMEABILITY; MEMBRANE POTENTIAL; NONHUMAN; SPORE GERMINATION;

BACILLUS CEREUS; BACILLUS COAGULANS; BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); POSIBACTERIA;

EID: 1842453766     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2004.02184.x     Document Type: Article

References (29)

1. Barer, M.R. and Harwood, C.R. (1999) Bacterial viability and culturability. Advance in Microbial Physiology 41, 93-137.
2. Bhupathiraju, V.K., Hernandez, M., Landfear, D. and Alvarez-Cohen, L. (1999) Application of a tetrazolium dye as an indicator of viability in anaerobic bacteria. Journal of Microbiological Methods 37, 231-243.
3. Botha, S.J. and Holzapfel, W.H. (1988) Resistance of vegetative cells and endospores of Sporolactobacillus to gamma-irradiation. International Journal of Food Microbiology 7, 169-172.
4. Caramello, P., Mazzucco, G., Romeo, M., Ullio, A., DeRosa, G., Lucchini, A., Forno, B., Brancale, T. et al. (1995) Clinical and microscopical features of small-intestinal microsporidiosis in patients with AIDS. Infection 23, 362-368.
5. Comas-Riu, J. and Vives-Rego, J. (2002) Cytometric monitoring of growth, sporogenesis and spore cell sorting in Paenibacillus polymyxa (formerly Bacillus polymyxa). Journal of Applied Microbiology 92, 475-481.
6. -), for the flow cytometry assessment of bacterial viability. FEMS Microbiological Letters 130, 165-170.
7. Dragon, D.C. and Rennie, R.P. (1995) The ecology of anthrax spores: tough but not invicible. Canadian Veterinary Journal 36, 295-301.
8. Driks, A. (1999) Bacillus subtilis spore coat. Microbiology and Molecular Biology Reviews 63, 1-20.
9. Edwards, C. (2000) Problems posed by natural environments for monitoring microorganisms. Molecular Biotechnology 15, 211-223.
10. Frazier, W.C. and Westhoff, D.C. (1988) Preservation by radiation. In Food Microbiology, 4th edn. pp. 539. New York: McGraw-Hill.
11. Fry, J.C. (1990) Direct methods and biomass estimation. Methods in Microbiology 22, 41-85.
12. Gantotti, B.V. and Woodske, D.V. (1991) Nuclear staining of Colletotrichum gloeosporioides f. sp. Malvae conidia with fluorescent and nonfluorescent stains. Biotechnic and Histochemistry 1, 7-13.
13. Green, L.C., Leblanc, P.J. and Didier, E.S. (2000) Discrimination between viable and dead Encephalitizoon cuniculi (microsporidian) spores by dual staining with Sytox green and calcofluor with M2R. Journal of Clinical Microbiology 38, 3811-3814.
14. Han, Y.W. (1990) Microbial levan. Advances in Applied Microbiology 35, 171-194.
15. Ho, J. (2002) Future of biological aerosol detection. Analytica Chimica Acta 457, 125-148.
16. Ho, J., Spence, M. and Hairston, P. (1999) Measurement of biological aerosol with a fluorescent aerodynamic particle sizer (FLAPS): correlation of optical data with biological data. In Rapid Methods for Analysis of Biological Materials in the Environment ed. Stopa, P.J. and Bartoszcze, M.A., pp. 177-201. the Netherlands: Kluwer Academic Publishers.
17. Holt, J.G. and Krieg, N.R. (1994) Growth. Methods for general and molecular bacteriology. In Enrichment and Isolation ed. Gerhardt, P., Murray, R.G.E., Wood, W.A. and Krieg, N.R. pp. 179-215. Washington DC: American Society for Microbiology.
18. Inglesby, T.M., Henderson, D.A., Barlett, J.G., Ascher, M.S., Eitzen, E., Friedlander, A.M., Hauer, J., McDade, J. et al. (1999) Anthrax as a biological weapon. Medical and public health management. Journal of the American Medical Association 281, 1735-1745.
19. Kell, D.B., Kaprelyants, A.S., Weichart, D.H., Harwood C.R. and Barer, M.R. (1998) Viability and activity in readily culturable bacteria: a review and discussion of the pratical issues. Antonie Van Leeuwenhoek 73, 169-187.
20. Nebe-von-Caron, G., Stephens, P.J., Hewitt, C.J., Powell, J.R. and Badley, R.A. (2000) Analysis of bacterial function by multi-colour fluorescence flow cytometry and single cell sorting. Journal of Microbiological Methods 42, 97-114.
21. Nicholson, W.L. and Law, J.F. (1999) Method for purification of bacterial endospores from soils: UV resistance of natural Sonoran desert soil populations of Bacillus sp. with reference to B. subtilis strain 168. Journal of Microbiological Methods 35, 13-21.
22. Nicholson, W.L., Munakata, N., Horneck, G., Melosh, H.J. and Setlow, P. (2000) Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments. Microbiology and Molecular Biology Reviews 64, 548-572.
23. Nishihara, T.Y., Takubo, E., Kawamata, T., Koshikawa, J., Ogaki, J. and Kondo, M. (1989) Role of outer coat in resistance of Bacillus megaterium spore. Journal of Biochemistry 106, 270-273.
24. Ogbadu, G. (1981) Ultrastructural changes in gamma-irradiated Aspergillus flavus spores. Cytobios 30, 167-171.
25. Rodriguez, G.G., Phipps, D., Ishiguro, K. and Ridgway, H.F. (1992) Use of a fluorescent redox probe for direct visualisation of actively respiring bacteria. Applied and Environmental Microbiology 58, 1801-1808.
26. Russel, A.D. (1990) Bacterial spore and chemical sporocidal agents. Clinical Microbiology Reviews 3, 99-119.
27. Scherrer, R., Beaman, T.C. and Gerhardt, P. (1971) Macromolecular sieving by the dormant spore of Bacillus cereus. Journal of Bacteriology 108, 868-873.
28. Stopa, P.J. (2000) The flow cytometry of Bacillus anthracis spores revisited. Cytometry 41, 237-244.
29. Williams, R.P. (1986) Infectious disease and medical microbiology. In Bacillus anthracis and Other Spore Forming Bacilli ed. Braude, A., Davis, L.E. and Fierer, J. pp. 270-278. Phidadelphia, PA: WB Saunders Co.