Extraction of nucleic acids from bacterial spores using bead-based mechanical lysis on a plastic chip

Engineering in Life Sciences

Volumn 11, Issue 2, 2011, Pages 174-181

  Geissler, Matthias ^a   Beauregard, Julie A ^a,b   Charlebois, Isabelle ^b   Isabel, Sandra ^b   Normandin, François ^a   Voisin, Benoît ^a   Boissinot, Maurice ^b   Bergeron, Michel G ^b   Veres, Teodor ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b LAVAL UNIVERSITY   (Canada)

Author keywords

Bacterial spores; DNA extraction; Mechanical cell lysis; Microfluidics; PCR

Indexed keywords

ACID EXTRACTION; BACILLUS ATROPHAEUS; BACTERIAL SPORE; CELL DISRUPTION; DNA EXTRACTION; FRICTIONAL FORCES; GENETIC MATERIALS; GENOMIC DNA; INSTRUMENTAL PARAMETERS; LYSATES; MAGNETIC ACTUATION; MATRIX; MECHANICAL CELLS; METAL DISK; MICROBEADS; PCR; PLASTIC CHIPS; REAL-TIME PCR;

ACTUATOR DISKS; BACTERIOLOGY; DNA; MICROFLUIDICS; NUCLEIC ACIDS; POLYMERASE CHAIN REACTION; THERMAL VARIABLES MEASUREMENT;

EXTRACTION;

BACTERIAL DNA; NUCLEIC ACID; POLY(METHYL METHACRYLATE);

BACTERIUM; BIOCHEMISTRY; CYTOLOGY; EFFICIENCY MEASUREMENT; EXTRACTION METHOD; FILM; FLUID MECHANICS; LYSIS; MAGNETIC METHOD; NUCLEIC ACID; PARAMETERIZATION; POLYMER; POLYMERASE CHAIN REACTION; SPORE;

ARTICLE; BACILLUS; BACILLUS ATROPHAEUS; BACTERIAL GENETICS; BACTERIAL SPORE; CELL DISRUPTION; DNA EXTRACTION; FLUORESCENCE; FRICTION; MAGNETISM; MICROFLUIDICS; NONHUMAN; POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION;

BACILLUS ATROPHAEUS; BACTERIA (MICROORGANISMS);

EID: 79955372769     PISSN: 16180240     EISSN: 16182863     Source Type: Journal    
DOI: 10.1002/elsc.201000132     Document Type: Article

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