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High throughput sequencing reveals a complex pattern of dynamic interrelationships among human T cell subsets
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Using high-throughput sequencing, this study addressed both TRB and TRA repertoires of different CD4 and CD8 T-cell subpopulations
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Wang C, Sanders CM, Yang Q, et al. High throughput sequencing reveals a complex pattern of dynamic interrelationships among human T cell subsets. Proc Natl Acad Sci U S A 2010; 107:1518-1523. Using high-throughput sequencing, this study addressed both TRB and TRA repertoires of different CD4 and CD8 T-cell subpopulations.
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(2010)
Proc Natl Acad Sci U S A
, vol.107
, pp. 1518-1523
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Wang, C.1
Sanders, C.M.2
Yang, Q.3
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42
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77950541195
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Comprehensive assessment of T-cell receptor beta-chain diversity in alphabeta T cells
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Here, the TCR repertoire diversity is analyzed in naive and Ag-experienced CD4 and CD8 T cells isolated ex vivo. Compared with previous two studies [40,41] based on mRNA/cDNA sequencing, this study presents the advantage to address the TCR sequences at the DNA level allowing an evaluation of their relative frequency within T-cell population. On the basis of sequences analysis and unseen species model, the entire TRB repertoire was estimated at 3-4 million CDR3 sequences
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Robins HS, Campregher PV, Srivastava SK, et al. Comprehensive assessment of T-cell receptor beta-chain diversity in alphabeta T cells. Blood 2009; 114:4099-4107. Here, the TCR repertoire diversity is analyzed in naive and Ag-experienced CD4 and CD8 T cells isolated ex vivo. Compared with previous two studies [40,41] based on mRNA/cDNA sequencing, this study presents the advantage to address the TCR sequences at the DNA level allowing an evaluation of their relative frequency within T-cell population. On the basis of sequences analysis and unseen species model, the entire TRB repertoire was estimated at 3-4 million CDR3 sequences.
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(2009)
Blood
, vol.114
, pp. 4099-4107
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Robins, H.S.1
Campregher, P.V.2
Srivastava, S.K.3
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43
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77956460339
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+ T cell receptor repertoire
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This study revealed a large interindividual overlap of TCR repertoire and described several mechanisms governing the generation of CDR3 sequences and their selection in naive and memory T-cell compartments
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+ T cell receptor repertoire. Sci Transl Med 2010; 2:47ra64. This study revealed a large interindividual overlap of TCR repertoire and described several mechanisms governing the generation of CDR3 sequences and their selection in naive and memory T-cell compartments.
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(2010)
Sci Transl Med
, vol.2
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Robins, H.S.1
Srivastava, S.K.2
Campregher, P.V.3
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44
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79955022207
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A mechanism for TCR sharing between T cell subsets and individuals revealed by pyrosequencing
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One TRBV-TRBJ rearrangement was analyzed by deep sequencing in naive and memory T cells from four individuals. More than 100 000 and 200 000 sequences were obtained in each compartment, respectively. A high degree of overlap was observed between individuals and between T-cell populations within one individual. The degree of CDR3 sequence sharing was correlated with its frequency in both naive and memory pool, which is influenced by convergent recombination
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Venturi V, Quigley MF, Greenaway HY, et al. A mechanism for TCR sharing between T cell subsets and individuals revealed by pyrosequencing. J Immunol 2011; 186:4285-4294. One TRBV-TRBJ rearrangement was analyzed by deep sequencing in naive and memory T cells from four individuals. More than 100 000 and 200 000 sequences were obtained in each compartment, respectively. A high degree of overlap was observed between individuals and between T-cell populations within one individual. The degree of CDR3 sequence sharing was correlated with its frequency in both naive and memory pool, which is influenced by convergent recombination.
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(2011)
J Immunol
, vol.186
, pp. 4285-4294
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Venturi, V.1
Quigley, M.F.2
Greenaway, H.Y.3
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