Optimisation of a triplex real time RT-PCR for detection of hepatitis E virus RNA and validation on biological samples

Journal of Virological Methods

Volumn 180, Issue 1-2, 2012, Pages 38-42

  Vasickova, Petra ^a   Kralik, Petr ^a   Slana, Iva ^a   Pavlik, Ivo ^a  

^a VETERINARY RESEARCH INSTITUTE   (Czech Republic)

Author keywords

Internal amplification control; RNA quantitation; Zoonosis

Indexed keywords

VIRUS RNA;

ARTICLE; COHORT ANALYSIS; CONTROLLED STUDY; DEER; DILUTION; GENE AMPLIFICATION; GENE LOCUS; HEPATITIS E VIRUS; INTERMETHOD COMPARISON; LIMIT OF DETECTION; NESTED REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SENSITIVITY AND SPECIFICITY; SHEEP; STANDARD; TRIPLEX REAL TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS DETECTION; WILD BOAR;

ANIMALS; DEER; HEPATITIS E; HEPATITIS E VIRUS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SHEEP; SUS SCROFA;

ANIMALIA; DAMA DAMA; HEPATITIS E VIRUS; OVIS ARIES MUSIMON; SUS SCROFA;

EID: 84857041696     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2011.12.007     Document Type: Article

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