Comparative analysis of different TaqMan real-time RT-PCR assays for the detection of swine Hepatitis e virus and integration of Feline calicivirus as internal control

Journal of Applied Microbiology

Volumn 106, Issue 4, 2009, Pages 1360-1369

  Ward, P ^a   Poitras, E ^a   Leblanc, D ^a   Letellier, A ^b   Brassard, J ^a   Plante, D ^c   Houde, A ^a  

^a FOOD RESEARCH AND DEVELOPMENT CENTRE   (Canada)

^b UNIVERSITÉ DE MONTRÉAL   (Canada)

^c HEALTH CANADA   (Canada)

Author keywords

Feline calicivirus; Internal control; Nested RT PCR; Swine Hepatitis E virus; TaqMan real time RT PCR

Indexed keywords

EXTRACTION; FINANCE; POLYMERASE CHAIN REACTION; RNA; VIRUSES;

FAECAL SAMPLES; FELINE CALICIVIRUS; HEPATITIS E VIRUS; INTERNAL CONTROLS; NEGATIVE SAMPLES; NESTED RT-PCR; PCR ASSAY; REAL TIME RT-PCR; SWINE HEPATITIS E VIRUS; TAQMAN REAL-TIME RT-PCR;

ASSAYS;

VIRUS RNA;

BIOASSAY; BLOOD; COMPARATIVE STUDY; DETECTION METHOD; FECES; HEPATITIS; INOCULATION; PERFORMANCE ASSESSMENT; PIG; POLYMERASE CHAIN REACTION; REAL TIME; RNA; VIRUS;

ANALYTICAL EQUIPMENT; ARTICLE; BLOOD ANALYSIS; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; FECES ANALYSIS; HEPATITIS E VIRUS; INOCULATION; INTERMETHOD COMPARISON; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMA; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SWINE; VESIVIRUS; VIRAL CONTAMINATION; VIRUS DETECTION;

EID: 62149149108     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2008.04104.x     Document Type: Article

References (46)

1. Aggarwal, R. Krawczynski, K. (2000) Hepatitis E: an overview and recent advances in clinical and laboratory research. J Gastroenterol Hepatol 15, 9 20.
2. Ahn, J.M., Rayamajhi, N., Gyun Kang, S. Sang Yoo, H. (2006) Comparison of real-time reverse transcriptase-polymerase chain reaction and nested or commercial reverse transcriptase-polymerase chain reaction for the detection of hepatitis E virus particle in human serum. Diagn Microbiol Infect Dis 56, 269 274.
3. Balayan, M.S., Andjaparidze, A.G., Savinskaya, S.S., Ketiladze, E.S., Braginsky, D.M., Savinov, A.P. Poleschuk, V.F. (1983) Evidence for a virus in non-A, non-B hepatitis transmitted via the fecal-oral route. Intervirology 20, 23 31.
4. Bidawid, S., Malik, N., Adegbunrin, O., Sattar, S.A. Farber, J.M. (2003) A feline kidney cell line-based plaque assay for feline calicivirus, a surrogate for Norwalk virus. J Virol Methods 107, 163 167.
5. Caron, M., Enouf, V., Than, S.C., Dellamonica, L., Buisson, Y. Nicand, E. (2006) Identification of genotype 1 hepatitis E virus in samples from swine in Cambodia. J Clin Microbiol 44, 3440 3442.
6. Chobe, L.P., Lole, K.S. Arankalle, V.A. (2006) Full genome sequence and analysis of Indian swine hepatitis E virus isolate of genotype 4. Vet Microbiol 114, 240 251.
7. Choi, I.S., Kwon, H.J., Shin, N.R. Yoo, H.S. (2003) Identification of swine hepatitis E virus (HEV) and prevalence of anti-HEV antibodies in swine and human populations in Korea. J Clin Microbiol 41, 3602 3608.
8. Cooper, K., Huang, F.F., Batista, L., Rayo, C.D., Bezanilla, J.C., Toth, T.E. Meng, X.J. (2005) Identification of genotype 3 hepatitis E virus (HEV) in serum and fecal samples from pigs in Thailand and Mexico, where genotype 1 and 2 HEV strains are prevalent in the respective human populations. J Clin Microbiol 43, 1684 1688.
9. Emerson, S.U. Purcell, R.H. (2003) Hepatitis E virus. Rev Med Virol 13, 145 154.
10. Emerson, S.U., Anderson, D., Arankalle, A., Meng, X.J., Purdy, M., Schauder, G.G. Tsarev, S.A. (2005) Hepevirus. In Virus taxonomy, Eighth Report of the International Committee on Taxonomy of Viruses ed. Fauquet, C.M., Mayo, M.A., Maniloff, J., Desselberger, U. Ball, L.A. pp. 853 857. London : Elsevier/Academic Press.
11. Enouf, V., Dos Reis, G., Guthmann, J.P., Guerin, P.J., Caron, M., Marechal, V. Nicand, E. (2006) Validation of single real-time TaqMan PCR assay for the detection and quantitation of four major genotypes of hepatitis E virus in clinical specimens. J Med Virol 78, 1076 1082.
12. Escobar-Herrera, J., Cancio, C., Guzman, G.I., Villegas-Sepulveda, N., Estrada-Garcia, T., Garcia-Lozano, H., Gomez-Santiago, F. Gutierrez-Escolano, A.L. (2006) Construction of an internal RT-PCR standard control for the detection of human caliciviruses in stool. J Virol Methods 137, 334 338.
13. Feagins, A.R., Opriessnig, T., Guenette, D.K., Halbur, P.G. Meng, X.J. (2007) Detection and characterization of infectious Hepatitis E virus from commercial pig livers sold in local grocery stores in the USA. J Gen Virol 88, 912 917.
14. Gyarmati, P., Mohammed, N., Norder, H., Blomberg, J., Belak, S. Widen, F. (2007) Universal detection of hepatitis E virus by two real-time PCR assays: TaqMan and Primer-Probe Energy Transfer. J Virol Methods 146, 226 235.
15. Huang, F.F., Haqshenas, G., Guenette, D.K., Halbur, P.G., Schommer, S.K., Pierson, F.W., Toth, T.E. Meng, X.J. (2002) Detection by reverse transcription-PCR and genetic characterization of field isolates of swine hepatitis E virus from pigs in different geographic regions of the United States. J Clin Microbiol 40, 1326 1332.
16. Innis, B.L., Seriwatana, J., Robinson, R.A., Shrestha, M.P., Yarbough, P.O., Longer, C.F., Scott, R.M., Vaughn, D.W. et al. (2002) Quantitation of immunoglobulin to hepatitis E virus by enzyme immunoassay. Clin Diagn Lab Immunol 9, 639 648.
17. Inoue, J., Takahashi, M., Yazaki, Y., Tsuda, F. Okamoto, H. (2006) Development and validation of an improved RT-PCR assay with nested universal primers for detection of hepatitis E virus strains with significant sequence divergence. J Virol Methods 137, 325 333.
18. Jothikumar, N., Paulmurugan, R., Padmanabhan, P., Sundari, R.B., Kamatchiammal, S. Rao, K.S. (2000) Duplex RT-PCR for simultaneous detection of hepatitis A and hepatitis E virus isolated from drinking water samples. J Environ Monit 2, 587 590.
19. Jothikumar, N., Cromeans, T.L., Robertson, B.H., Meng, X.J. Hill, V.R. (2006) A broadly reactive one-step real-time RT-PCR assay for rapid and sensitive detection of hepatitis E virus. J Virol Methods 131, 65 71.
20. Leblanc, D., Ward, P., Gagne, M.J., Poitras, E., Muller, P., Trottier, Y.L., Simard, C. Houde, A. (2007) Presence of hepatitis E virus in a naturally infected swine herd from nursery to slaughter. Int J Food Microbiol 117, 160 166.
21. Li, T.C., Chijiwa, K., Sera, N., Ishibashi, T., Etoh, Y., Shinohara, Y., Kurata, Y., Ishida, M. et al. (2005) Hepatitis E virus transmission from wild boar meat. Emerg Infect Dis 11, 1958 1960.
22. Lu, L., Li, C. Hagedorn, C.H. (2006) Phylogenetic analysis of global hepatitis E virus sequences: genetic diversity, subtypes and zoonosis. Rev Med Virol 16, 5 36.
23. Mansuy, J.M., Peron, J.M., Abravanel, F., Poirson, H., Dubois, M., Miedouge, M., Vischi, F., Alric, L. et al. (2004) Hepatitis E in the south west of France in individuals who have never visited an endemic area. J Med Virol 74, 419 424.
24. Mattison, K., Brassard, J., Houde, A., Simard, C., Pagotto, F., Jones, T. Trottier, Y.-L. Octobre (2007) The Feline Calicivirus (FCV) As an Internal Control for the Detection of RNA Viruses from Foods. Federal Food Safety and Nutrition Research Meeting. Winnipeg, Manitoba, Canada.
25. Meng, X.J., Purcell, R.H., Halbur, P.G., Lehman, J.R., Webb, D.M., Tsareva, T.S., Haynes, J.S., Thacker, B.J. et al. (1997) A novel virus in swine is closely related to the human hepatitis E virus. Proc Natl Acad Sci USA 94, 9860 9865.
26. Meng, X.J., Halbur, P.G., Shapiro, M.S., Govindarajan, S., Bruna, J.D., Mushahwar, I.K., Purcell, R.H. Emerson, S.U. (1998) Genetic and experimental evidence for cross-species infection by swine hepatitis E virus. J Virol 72, 9714 9721.
27. Meng, X.J., Dea, S., Engle, R.E., Friendship, R., Lyoo, Y.S., Sirinarumitr, T., Urairong, K., Wang, D. et al. (1999) Prevalence of antibodies to the hepatitis E virus in pigs from countries where hepatitis E is common or is rare in the human population. J Med Virol 59, 297 302.
28. Meng, X.J., Wiseman, B., Elvinger, F., Guenette, D.K., Toth, T.E., Engle, R.E., Emerson, S.U. Purcell, R.H. (2002) Prevalence of antibodies to hepatitis E virus in veterinarians working with swine and in normal blood donors in the United States and other countries. J Clin Microbiol 40, 117 122.
29. Mizuo, H., Yazaki, Y., Sugawara, K., Tsuda, F., Takahashi, M., Nishizawa, T. Okamoto, H. (2005) Possible risk factors for the transmission of hepatitis E virus and for the severe form of hepatitis E acquired locally in Hokkaido, Japan. J Med Virol 76, 341 349.
30. Mushahwar, I.K. (2008) Hepatitis E virus: molecular virology, clinical features, diagnosis, transmission, epidemiology, and prevention. J Med Virol 80, 646 658.
31. Nishizawa, T., Takahashi, M., Mizuo, H., Miyajima, H., Gotanda, Y. Okamoto, H. (2003) Characterization of Japanese swine and human hepatitis E virus isolates of genotype IV with 99% identity over the entire genome. J Gen Virol 84, 1245 1251.
32. Okamoto, H. (2007) Genetic variability and evolution of hepatitis E virus. Virus Res 127, 216 228.
33. Orru, G., Masia, G., Romano, L., Piras, V. Coppola, R.C. (2004) Detection and quantitation of hepatitis E virus in human faeces by real-time quantitative PCR. J Virol Methods 118, 77 82.
34. Renou, C., Cadranel, J.F., Bourliere, M., Halfon, P., Ouzan, D., Rifflet, H., Carenco, P., Harafa, A. et al. (2007) Possible zoonotic transmission of hepatitis E from pet pig to its owner. Emerg Infect Dis 13, 1094 1096.
35. Rutjes, S.A., Lodder, W.J., Bouwknegt, M. de Roda Husman, A.M. (2007) Increased hepatitis E virus prevalence on Dutch pig farms from 33 to 55% by using appropriate internal quality controls for RT-PCR. J Virol Methods 143, 112 116.
36. Scipioni, A., Bourgot, I., Mauroy, A., Ziant, D., Saegerman, C., Daube, G. Thiry, E. (2008) Detection and quantification of human and bovine noroviruses by a TaqMan RT-PCR assay with a control for inhibition. Mol Cell Probes 22, 215 222.
37. Smith, J.L. (2001) A review of hepatitis E virus. J Food Prot 64, 572 586.
38. Takahashi, M., Nishizawa, T., Miyajima, H., Gotanda, Y., Iita, T., Tsuda, F. Okamoto, H. (2003) Swine hepatitis E virus strains in Japan form four phylogenetic clusters comparable with those of Japanese isolates of human hepatitis E virus. J Gen Virol 84, 851 862.
39. Takahashi, M., Nishizawa, T., Tanaka, T., Tsatsralt-Od, B., Inoue, J. Okamoto, H. (2005) Correlation between positivity for immunoglobulin A antibodies and viraemia of swine hepatitis E virus observed among farm pigs in Japan. J Gen Virol 86, 1807 1813.
40. Tam, A.W., Smith, M.M., Guerra, M.E., Huang, C.C., Bradley, D.W., Fry, K.E. Reyes, G.R. (1991) Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome. Virology 185, 120 131.
41. Tei, S., Kitajima, N., Takahashi, K. Mishiro, S. (2003) Zoonotic transmission of hepatitis E virus from deer to human beings. Lancet 362, 371 373.
42. Wang, Y., Zhang, H., Li, Z., Gu, W., Lan, H., Hao, W., Ling, R., Li, H. et al. (2001) Detection of sporadic cases of hepatitis E virus (HEV) infection in China using immunoassays based on recombinant open reading frame 2 and 3 polypeptides from HEV genotype 4. J Clin Microbiol 39, 4370 4379.
43. Ward, P., Muller, P., Letellier, A., Quessy, S., Simard, C., Trottier, Y.L., Houde, A. Brassard, J. (2008) Molecular characterization of hepatitis E virus detected in swine farms in the province of Quebec. Can J Vet Res 72, 27 31.
44. Williams, T.P., Kasorndorkbua, C., Halbur, P.G., Haqshenas, G., Guenette, D.K., Toth, T.E. Meng, X.J. (2001) Evidence of extrahepatic sites of replication of the hepatitis E virus in a swine model. J Clin Microbiol 39, 3040 3046.
45. Yazaki, Y., Mizuo, H., Takahashi, M., Nishizawa, T., Sasaki, N., Gotanda, Y. Okamoto, H. (2003) Sporadic acute or fulminant hepatitis E in Hokkaido, Japan, may be food-borne, as suggested by the presence of hepatitis E virus in pig liver as food. J Gen Virol 84, 2351 2357.
46. Yoo, D., Willson, P., Pei, Y., Hayes, M.A., Deckert, A., Dewey, C.E., Friendship, R.M., Yoon, Y. et al. (2001) Prevalence of hepatitis E virus antibodies in Canadian swine herds and identification of a novel variant of swine hepatitis E virus. Clin Diagn Lab Immunol 8, 1213 1219.