Tissue preparation and immunostaining of mouse sensory nerve fibers innervating skin and limb bones

Journal of Visualized Experiments

Volumn , Issue 59, 2012, Pages 1-6

  Shepherd, Andrew J ^a   Mohapatra, Durga P ^a,b  

^a UNIVERSITY OF IOWA   (United States)

^b UNIVERSITY OF IOWA   (United States)

Author keywords

Bone; CGRP; Immunostaining; Issue 59; Neuroscience; NF200; Pain; Plantar punch; Sensory nerve fiber; Skin; TRPV1; Tubulin

Indexed keywords

EID: 84856977683     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3485     Document Type: Article

References (12)

1. Hoffman, E.M., Schechter, R., & Miller, K.E. Fixative Composition Alters Distributions of Immunoreactivity for Glutaminase and Two Markers of Nociceptive Neurons, Nav 1.8 and TRPV1, in the Rat Dorsal Root Ganglion. Journal of Histochemistry & Cytochemistry. 58, 329-344 (2010).
2. Neves, J.D.S., Omar, N.F., Narvaes, E.A.O., Gomes, J.R., & Novaes, P.D. Influence of different decalcifying agents on EGF and EGFR immunostaining. Acta Histochemica. 113, 484-488 (2011).
3. Watson, R.E., Jr., Wiegand, S.J., Clough, R.W., & Hoffman, G.E. Use of cryoprotectant to maintain long-term peptide immunoreactivity and tissue morphology. Peptides. 7, 155-159 (1986).
4. Yen, L.D., Bennett, G.J., & Ribeiro-da-Silva, A. Sympathetic sprouting and changes in nociceptive sensory innervation in the glabrous skin of the rat hind paw following partial peripheral nerve injury. The Journal of Comparative Neurology. 495, 679-690 (2006).
5. Boyette-Davis, J., Xin, W., Zhang, H., & Dougherty, P.M. Intraepidermal nerve fiber loss corresponds to the development of Taxol-induced hyperalgesia and can be prevented by treatment with minocycline. Pain. 152, 308-313 (2011).
6. Bloom, A.P., et al. Breast Cancer-Induced Bone Remodeling, Skeletal Pain, and Sprouting of Sensory Nerve Fibers. The Journal of Pain. 12, 698-711 (2011).
7. Constantin, C.E., et al. Endogenous Tumor Necrosis Factor α (TNFα) Requires TNF Receptor Type 2 to Generate Heat Hyperalgesia in a Mouse Cancer Model. J. Neurosci. 28, 5072-5081 (2008).
8. Jankowski, M.P. et al. Sensitization of Cutaneous Nociceptors after Nerve Transection and Regeneration: Possible Role of Target-Derived Neurotrophic Factor Signaling. The Journal of Neuroscience. 29, 1636-1647 (2009).
9. Jimenez-Andrade, J.M., et al. Pathological Sprouting of Adult Nociceptors in Chronic Prostate Cancer-Induced Bone Pain. J. Neurosci. 30, 14649-14656 (2010).
10. Persson, A.-K., et al. Sodium-calcium exchanger and multiple sodium channel isoforms in intra-epidermal nerve terminals. Molecular Pain. 6, 84 (2010).
11. Ohshima, M., Miyake, M., Takeda, M., Kamijima, M., & Sakamoto, T. Staphylococcal Enterotoxin B Causes Proliferation of Sensory C-Fibers and Subsequent Enhancement of Neurogenic Inflammation in Rat Skin. Journal of Infectious Diseases. 203, 862-869 (2011).
12. Johnson, M.S., Ryals, J.M., & Wright, D.E. Early loss of peptidergic intraepidermal nerve fibers in an STZ-induced mouse model of insensate diabetic neuropathy. Pain. 140, 35-47 (2008).