Fixative composition alters distributions of immunoreactivity for glutaminase and two markers of nociceptive neurons, Nav1.8 and TRPV1, in the rat dorsal root ganglion

Journal of Histochemistry and Cytochemistry

Volumn 58, Issue 4, 2010, Pages 329-344

  Hoffman, E Matthew ^a   Schechter, Ruben ^a   Miller, Kenneth E ^a  

^a OKLAHOMA STATE UNIVERSITY   (United States)

Author keywords

Glutamate; Image analysis; ImageJ; Immunohistochemistry; Paraformaldehyde; Phosphate activated glutaminase; Primary afferent neurons

Indexed keywords

FORMALDEHYDE; GLUTAMINASE; PICRIC ACID; VANILLOID RECEPTOR 1; VOLTAGE GATED SODIUM CHANNEL;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; IMMUNOFLUORESCENCE; IMMUNOREACTIVITY; INCUBATION TIME; NERVE CELL; NOCICEPTIVE RECEPTOR; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SPINAL GANGLION;

ANIMALS; BIOLOGICAL MARKERS; FIXATIVES; FLUORESCENT ANTIBODY TECHNIQUE; FORMALDEHYDE; GANGLIA, SPINAL; GLUTAMINASE; IMMUNE SERA; NERVE TISSUE PROTEINS; NOCICEPTORS; PICRATES; RATS; RATS, SPRAGUE-DAWLEY; SODIUM CHANNELS; TIME FACTORS; TISSUE FIXATION; TRPV CATION CHANNELS;

RATTUS;

EID: 77949785244     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.2009.954008     Document Type: Article

References (43)

1. Akopian AN, Sivilotti L, Wood JN (1996) A tetrodotoxin-resistant voltage-gated sodium channel expressed by sensory neurons. Nature 379:257-262
2. Battaglia G, Rustioni A (1988) Coexistence of glutamate and substance P in dorsal root ganglion neurons of the rat and monkey. J Comp Neurol 277:302-312
3. v1.9 (SNS2) in primary sensory neurons. J Neurosci 21:6077-6085
4. Blum F (1893) Der formaldehyd als hartungsmittel. Z Wiss Mikrosk 10:314-315
5. Brumovsky P, Watanabe M, Hokfelt T (2007) Expression of the vesicular glutamate transporters-1 and -2 in adult mouse dorsal root ganglia and spinal cord and their regulation by nerve injury. Neuroscience 147:469-490
6. Cangro CB, Sweetnam PM, Neale JH, Haser WG, Curthoys NP (1984) Selective localization of glutaminase in spinal and sensory nerve cells. A potential marker for glutamate neurotransmission. JAMA 251:797
7. Cangro CB, Sweetnam PM, Wrathall JR, Haser WB, Curthoys NP, Neale JH (1985) Localization of elevated glutaminase immunoreactivity in small DRG neurons. Brain Res 336:158-161
8. Caterina MJ, Schumacher MA, Tominaga M, Rosen TA, Levine JD, Julius D (1997) The capsaicin receptor: a heat-activated ion channel in the pain pathway. Nature 389:816-824
9. De Biasi S, Rustioni A (1990) Ultrastructural immunocytochemical localization of excitatory amino acids in the somatosensory system. J Histochem Cytochem 38:1745-1754
10. deGroot J, Zhou S, Carlton SM (2000) Peripheral glutamate release in the hindpaw following low and high intensity sciatic stimulation. Neuroreport 11:497-502
11. Djouhri L, Fang X, Okuse K, Wood JN, Berry CM, Lawson SN (2003) The TTX-resistant sodium channel Nav1.8 (SNS/PN3): expression and correlation with membrane properties in rat nociceptive primary afferent neurons. J Physiol 550:739-752
12. Dmitrieva N, Rodriguez-Malaver AJ, Perez J, Hernandez L (2004) Differential release of neurotransmitters from superficial and deep layers of the dorsal horn in response to acute noxious stimulation and inflammation of the rat paw. Eur J Pain 8:245-252
13. Fang X, Djouhri L, McMullan S, Berry C, Okuse K, Waxman SG, Lawson SN (2005a) trkA is expressed in nociceptive neurons and influences electrophysiological properties via Nav1.8 expression in rapidly conducting nociceptors. J Neurosci 25:4868-4878
14. Fang X, Djouhri L, McMullan S, Berry C, Waxman SG, Okuse K, Lawson SN (2006) Intense isolectin-B4 binding in rat dorsal root ganglion neurons distinguishes C-fiber nociceptors with broad action potentials and high Nav1.9 expression. J Neurosci 26:7281-7292
15. Fang X, McMullan S, Lawson SN, Djouhri L (2005b) Electrophysiological differences between nociceptive and non-nociceptive dorsal root ganglion neurones in the rat in vivo. J Physiol 565:927-943
16. Fox CH, Johnson FB, Whiting J, Roller PP (1985) Formaldehyde fixation. J Histochem Cytochem 33:845-853
17. Fraenkel-Conrat H, Mecham DK (1949) The reaction of formaldehyde with proteins; demonstration of intermolecular cross-linking by means of osmotic pressure measurements. J Biol Chem 177:477-486
18. Fraenkel-Conrat H, Olcott HS (1948a) The reaction of formaldehyde with proteins; cross-linking between amino and primary amide or guanidyl groups. J Am Chem Soc 70:2673-2684
19. Fraenkel-Conrat H, Olcott HS (1948b) Reaction of formaldehyde with proteins; cross-linking of amino groups with phenol, imidazole, or indole groups. J Biol Chem 174:827-843
20. Fukuoka T, Kobayashi K, Yamanaka H, Obata K, Dai Y, Noguchi K (2008) Comparative study of the distribution of the alpha-subunits of voltage-gated sodium channels in normal and axotomized rat dorsal root ganglion neurons. J Comp Neurol 510:188-206
21. Garry MG, Miller KE, Seybold VS (1989) Lumbar dorsal root ganglia of the cat: a quantitative study of peptide immunoreactivity and cell size. J Comp Neurol 284:36-47
22. Guo A, Vulchanova L, Wang J, Li X, Elde R (1999) Immunocytochemical localization of the vanilloid receptor 1 (VR1): relationship to neuropeptides, the P2X3 purinoceptor and IB4 binding sites. Eur J Neurosci 11:946-958
23. Haser WG, Shapiro RA, Curthoys NP (1985) Comparison of the phosphate-dependent glutaminase obtained from rat brain and kidney. Biochem J 229:399-408
24. Hopwood D (2002) Fixation and fixatives. In Bancroft JD, Gamble M, eds. Theory and Practice of Histological Techniques. London, Churchill Livingstone, 63-84
25. Jin YH, Nishioka H, Wakabayashi K, Fujita T, Yonehara N (2006) Effect of morphine on the release of excitatory amino acids in the rat hind instep: pain is modulated by the interaction between the peripheral opioid and glutamate systems. Neuroscience 138:1329-1339
26. Kaneko T, Itoh K, Shigemoto R, Mizuno N (1989) Glutaminase-like immunoreactivity in the lower brainstem and cerebellum of the adult rat. Neuroscience 32:79-98
27. Kaneko T, Urade Y, Mizuno N (1988) Correlation between immunochemical characteristics and immunohistochemical applicability of nine lines of monoclonal antibodies against rat brain glutaminase. J Histochem Cytochem 36:997-1004
28. Lee KH, Chung K, Chung JM, Coggeshall RE (1986) Correlation of cell body size, axon size, and signal conduction velocity for individually labelled dorsal root ganglion cells in the cat. J Comp Neurol 243:335-346
29. Li JL, Fujiyama F, Kaneko T, Mizuno N (2003a) Expression of vesicular glutamate transporters, VGluT1 and VGluT2, in axon terminals of nociceptive primary afferent fibers in the superficial layers of the medullary and spinal dorsal horns of the rat. J Comp Neurol 457:236-249
30. Li JL, Ohishi H, Kaneko T, Shigemoto R, Neki A, Nakanishi S, Mizuno N (1996) Immunohistochemical localization of a metabotropic glutamate receptor, mGluR7, in ganglion neurons of the rat: with special reference to the presence in glutamatergic ganglion neurons. Neurosci Lett 204:9-12
31. Li JL, Xiong KH, Dong YL, Fujiyama F, Kaneko T, Mizuno N (2003b) Vesicular glutamate transporters, VGluT1 and VGluT2, in the trigeminal ganglion neurons of the rat, with special reference to coexpression. J Comp Neurol 463:212-220
32. Miller KE, Douglas VD, Kaneko T (1993) Glutaminase immunoreactive neurons in the rat dorsal root ganglion contain calcitonin gene-related peptide (CGRP). Neurosci Lett 160:113-116
33. NIH-NRC (2003) Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research/ Committee on Guidelines for the Use of Animals in Neuroscience and Behavioral Research. Washington, DC, The National Academic Press, 223
34. Polak JM, Van Noorden S (2003) Introduction to Immunocytochemistry. 3rd ed. Oxford, BIOS Scientific Publishers
35. Price TJ, Flores CM (2007) Critical evaluation of the colocalization between calcitonin gene-related peptide, substance P, transient receptor potential vanilloid subfamily type 1 immunoreactivities, and isolectin B4 binding in primary afferent neurons of the rat and mouse. J Pain 8:263-272
36. Sangameswaran L, Delgado SG, Fish LM, Koch BD, Jakeman LB, Stewart GR, Sze P, et al. (1996) Structure and function of a novel voltage-gated, tetrodotoxin-resistant sodium channel specific to sensory neurons. J Biol Chem 271:5953-5956
37. Shi SR, Key ME, Kalra KL (1991) Antigen retrieval in formalinfixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 39:741-748
38. Shi SR, Liu C, Taylor CR (2007) Standardization of immunohistochemistry for formalin-fixed, paraffin-embedded tissue sections based on the antigen-retrieval technique: from experiments to hypothesis. J Histochem Cytochem 55:105-109
39. Skilling SR, Smullin DH, Beitz AJ, Larson AA (1988) Extracellular amino acid concentrations in the dorsal spinal cord of freely moving rats following veratridine and nociceptive stimulation. J Neurochem 51:127-132
40. Sompuram SR, Vani K, Messana E, Bogen SA (2004) A molecular mechanism of formalin fixation and antigen retrieval. Am J Clin Pathol 121:190-199
41. Tuchscherer MM, Seybold VS (1985) Immunohistochemical studies of substance P, cholecystokinin-octapeptide and somatostatin in dorsal root ganglia of the rat. Neuroscience 14:593-605
42. Yoshida S, Matsuda Y (1979) Studies on sensory neurons of the mouse with intracellular-recording and horseradish peroxidaseinjection techniques. J Neurophysiol 42:1134-1145
43. Zahn PK, Sluka KA, Brennan TJ (2002) Excitatory amino acid release in the spinal cord caused by plantar incision in the rat. Pain 100:65-76