Influence of different decalcifying agents on EGF and EGFR immunostaining

Acta Histochemica

Volumn 113, Issue 4, 2011, Pages 484-488

  Neves, Juliana dos Santos ^a   Omar, Nádia Fayez ^a   Narvaes, Eliene Aparecida Orsini ^a   Gomes, José Rosa ^a   Novaes, Pedro Duarte ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

Decalcification; EGF; EGFR; Immunohistochemistry; Rat; Submandibular gland

Indexed keywords

EDETIC ACID; EPIDERMAL GROWTH FACTOR; EPIDERMAL GROWTH FACTOR RECEPTOR; FORMALDEHYDE; NITRIC ACID; TRICHLOROACETIC ACID;

ANIMAL TISSUE; ARTICLE; BONE DEMINERALIZATION; CONTROLLED STUDY; ENZYME ACTIVITY; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; MALE; MORPHOLOGY; NONHUMAN; PROCESS MODEL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; RAT; SUBMANDIBULAR GLAND; TISSUE STRUCTURE;

ANIMALS; DECALCIFICATION TECHNIQUE; EDETIC ACID; EPIDERMAL GROWTH FACTOR; FORMALDEHYDE; IMMUNOHISTOCHEMISTRY; MALE; MANDIBLE; NITRIC ACID; RATS; RATS, WISTAR; RECEPTOR, EPIDERMAL GROWTH FACTOR; SENSITIVITY AND SPECIFICITY; STAINING AND LABELING; SUBMANDIBULAR GLAND; TISSUE FIXATION; TRICHLOROACETIC ACID;

RATTUS; RATTUS NORVEGICUS;

EID: 79953767152     PISSN: 00651281     EISSN: 16180372     Source Type: Journal    
DOI: 10.1016/j.acthis.2010.04.006     Document Type: Article

References (21)

1. Alers J.C., Krijtenburg P., Vissers K.J., Dekken H.V. Effect of bone decalcification procedures on DNA in situ hybridization and comparative genomic hybridization: EDTA is highly preferable to a routinely used acid decalcifier. J Histochem Cytochem 1999, 47:703-709.
2. Arana-Chavez V.E., Nanci A. High-resolution immunocytochemistry of noncollagenous matrix proteins in rat mandibles processed with microwave irradiation. J Histochem Cytochem 2001, 49:1099-1109.
3. Athanassou N.A., Quinn J., Heryet A., Woods C.G., McGee J.O'.D. Effect of decalcification agents on immunoreactivity of cellular antigens. J Clin Pathol 1987, 40:874-878.
4. Bourque W.T., Gross M., Hall B.K. A histological processing technique that preserves the integrity of calcified tissues (bone, enamel), yolky amphibian embryos, and growth factor antigens in skeletal tissue. J Histochem Cytochem 1993, 41:1429-1434.
5. Carpenter G. Epidermal growth factor; biology and receptor metabolism. J Cell Sci 1985, 3:1-9.
6. Cohen S. Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new born animal. J Biol Chem 1962, 237:1555-1562.
7. Curran R.C., Gregory J. Effects of fixation and processing on immunohistochemical demonstration of immunoglobulin in paraffin sections of tonsil and bone marrow. J Clin Pathol 1980, 33:1047-1057.
8. Dubiel B., Mytar B., Tarnawski A., Zembala M., Stachura J. Epidermal growth factor (EGF) expression in human salivary glands. An immunohistochemical study. J Physiol Pharmacol 1992, 43:21-32.
9. Frank J.D., Balens R., Masarachia P., Seedor J.G., Cartwright M.E. The effects of three different demineralization agents on osteopontin localization in adult rat bone using immunohistochemistry. Histochemistry 1993, 99:295-301.
10. Graner E., Line S.R.P., Almeida O.P., Lopes M.A. Use of TCA as a decalcifying agent for laminin immunohistochemistry. Calcif Tissue Int 1995, 57:306.
11. Gresik E.W., Kashimata M., kadoya Y., Mathews R., Minami N., Yamashina S. Expression of epidermal growth factor receptor in fetal mouse submandibular gland detected by a biotinyltyramide-based catalyzed signal amplification method. J Histochem Cytochem 1997, 45:1651-1657.
12. Lantini M.S., Cossu M., Isola M., Piras M., Piludu M. Subcellular localization of epidermal growth factor receptor in human submandibular gland. J Anat 2006, 208:595-599.
13. Matthews J.B. Influence of decalcification on immunohistochemical staining of formalin-fixed paraffin-embedded tissue. J Clin Pathol 1982, 35:1392-1394.
14. Merchán-Pérez A., Gil-Loyzaga P., Bartolomé M.V., Remezal M., Fernández P., Rodríguez T. Decalcification by ascorbic acid for immuno- and affinohistochemical techniques on the inner ear. Histochem Cell Biol 1999, 112:125-130.
15. Mori S., Sawai T., Teshima T., Kyogoku M. A new decalcifying technique for immunohistochemical studies of calcified tissue, especially applicable to cell surface marker demonstration. J Histochem Cytochem 1988, 36:111-114.
16. Mukai K., Yoshimura S., Anzai M. Effects of decalcification on immunoperoxidase staining. Am J Surg Pathol 1986, 10:413-419.
17. Neves J.S., Salmon C.R., Omar N.F., Narvaes E.A.O., Gomes J.R., Novaes P.D. Immunolocalization of CSF-1, RANKL and OPG in the enamel-related periodontium of the rat incisor and their implications for alveolar bone remodeling. Arch Oral Biol 2009, 54:651-657.
18. Sanderson C., Radley K., Mayton L. Ethylenediaminetetraacetic acid in ammonium hydroxide for reducing decalcification time. Biotech Histochem 1995, 70:12-18.
19. Shi S., Coté C., Kalra K.L., Taylor C.R., Tandon A.K. A technique for retrieving antigens in formalin-fixed, routinely acid-decalcified, celloidin-embedded human temporal bone sections for immunohistochemistry. J Histochem Cytochem 1992, 40:787-792.
20. Suzuki H., Amizuka N., Noda M., Amano O., Maeda T. Histological and immunohistochemical changes in the submandibular gland in klotho-deficient mice. Arch Histol Cytol 2006, 69:119-128.
21. Walsh L., Freemont A.J., Hoyland J.A. The effect of tissue decalcification on mRNA retention within bone for in-situ hybridization studies. Int J Exp Pathol 1993, 64:237-241.