Conserved glycine 33 residue in flexible domain I of hepatitis C virus core protein is critical for virus infectivity

Journal of Virology

Volumn 86, Issue 2, 2012, Pages 679-690

  Angus, Allan G N ^a   Loquet, Antoine ^b,c   Stack, Séamus J ^a   Dalrymple, David ^a   Gatherer, Derek ^a   Penin, François ^b   Patel, Arvind H ^a  

^a MRC UNIVERSITY OF GLASGOW CENTRE FOR VIRUS RESEARCH   (United Kingdom)

^b INSTITUT DE BIOLOGIE ET CHIMIE DES PROTÉINES   (France)

^c MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; CORE PROTEIN; GLYCINE;

AMINO ACID SUBSTITUTION; ARTICLE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; HELIX LOOP HELIX MOTIF; HEPATITIS C VIRUS; HUMAN; HUMAN CELL; MOLECULAR DYNAMICS; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN MOTIF; PROTEIN STRUCTURE; VIRION; VIRUS GENOME; VIRUS INFECTIVITY; VIRUS REPLICATION;

AMINO ACID SEQUENCE; CELL LINE; CONSERVED SEQUENCE; GLYCINE; HEPACIVIRUS; HEPATITIS C; HUMANS; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ALIGNMENT; VIRAL CORE PROTEINS; VIRUS REPLICATION;

HEPATITIS C VIRUS;

EID: 84856947208     PISSN: 0022538X     EISSN: 10985514     Source Type: Journal    
DOI: 10.1128/JVI.05452-11     Document Type: Article

References (45)

1. Alsaleh K, et al. 2010. Identification of basic amino acids at the N-terminal end of the core protein that are crucial for hepatitis C virus infectivity. J. Virol. 84:12515-12528.
2. Angus AG, et al. 2010. Requirement of cellular DDX3 for hepatitis C virus replication is unrelated to its interaction with the viral core protein. J. Gen. Virol. 91:122-132.
3. Anonymous. 2004. Global burden of disease (GBD) for hepatitis C. J. Clin. Pharmacol. 44:20-29.
4. Barba G, et al. 1997. Hepatitis C virus core protein shows a cytoplasmic localization and associates to cellular lipid storage droplets. Proc. Natl. Acad. Sci. U. S. A. 94:1200-1205.
5. Bartenschlager R, Penin F, Lohmann V, Andre P. 2011. Assembly of infectious hepatitis C virus particles. Trends Microbiol. 19:95-103.
6. Boulant S, et al. 2006. Structural determinants that target the hepatitis C virus core protein to lipid droplets. J. Biol. Chem. 281:22236-22247.
7. Boulant S, Targett-Adams P, McLauchlan J. 2007. Disrupting the association of hepatitis C virus core protein with lipid droplets correlates with a loss in production of infectious virus. J. Gen. Virol. 88:2204-2213.
8. Boulant S, Vanbelle C, Ebel C, Penin F, Lavergne JP. 2005. Hepatitis C virus core protein is a dimeric alpha-helical protein exhibiting membrane protein features. J. Virol. 79:11353-11365.
9. Bukh J, Purcell RH, Miller RH. 1994. Sequence analysis of the core gene of 14 hepatitis C virus genotypes. Proc. Natl. Acad. Sci. U. S. A. 91: 8239-8243.
10. Chen SL, Morgan TR. 2006. The natural history of hepatitis C virus (HCV) infection. Int. J. Med. Sci. 3:47-52.
11. Clayton RF, et al. 2002. Analysis of antigenicity and topology of E2 glycoprotein present on recombinant hepatitis C virus-like particles. J. Virol. 76:7672-7682.
12. Cuevas JM, Gonzalez-Candelas F, Moya A, Sanjuan R. 2009. Effect of ribavirin on the mutation rate and spectrum of hepatitis C virus in vivo. J. Virol. 83:5760-5764.
13. de Chassey B, et al. 2008. Hepatitis C virus infection protein network. Mol. Syst. Biol. 4:230.
14. Duvignaud JB, Leclerc D, Gagne SM. 2010. Structure and dynamics changes induced by 2,2,2-trifluoro-ethanol (TFE) on the N-terminal half of hepatitis C virus core protein. Biochem. Cell Biol. 88:315-323.
15. Duvignaud JB, et al. 2009. Structure and dynamics of the N-terminal half of hepatitis C virus core protein: an intrinsically unstructured protein. Biochem. Biophys. Res. Commun. 378:27-31.
16. Flint M, et al. 1999. Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81. J. Virol. 73: 6235-6244.
17. Fujimoto Y, et al. 2004. Identification of major proteins in the lipid droplet-enriched fraction isolated from the human hepatocyte cell line HuH7. Biochim. Biophys. Acta 1644:47-59.
18. Gastaminza P, et al. 2010. Ultrastructural and biophysical characterization of hepatitis C virus particles produced in cell culture. J. Virol. 84: 10999-11009.
19. Gastaminza P, Kapadia SB, Chisari FV. 2006. Differential biophysical properties of infectious intracellular and secreted hepatitis C virus particles. J. Virol. 80:11074-11081.
20. Hope RG, McLauchlan J. 2000. Sequence motifs required for lipid droplet association and protein stability are unique to the hepatitis C virus core protein. J. Gen. Virol. 81:1913-1925.
21. Hüssy P, Langen H, Mous J, Jacobsen H. 1996. Hepatitis C virus core protein: carboxy-terminal boundaries of two processed species suggest cleavage by a signal peptide peptidase. Virology 224:93-104.
22. Iro M, et al. 2009. A reporter cell line for rapid and sensitive evaluation of hepatitis C virus infectivity and replication. Antiviral Res. 83:148-155.
23. Jolivet M, Penin F, Dalbon P, Ladaviere L, Lacoux X. 1997. Antigenic structural peptide, antigenic and immunogenic compounds and uses for detecting, preventing and treating an HCV infection. Patent application EP 1015481, WO 98/39360.
24. Koradi R, Billeter M, Wuthrich K. 1996. MOLMOL: a program for display and analysis of macromolecular structures. J. Mol. Graph. 14: 51-55, 29-32.
25. Lavanchy D. 2009. The global burden of hepatitis C. Liver Int. 29(Suppl. 1):74-81.
26. Lindenbach BD, et al. 2005. Complete replication of hepatitis C virus in cell culture. Science 309:623-626.
27. Masaki T, et al. 2008. Interaction of hepatitis C virus nonstructural protein 5A with core protein is critical for the production of infectious virus particles. J. Virol. 82:7964-7976.
28. McLauchlan J. 2000. Properties of the hepatitis C virus core protein: a structural protein that modulates cellular processes. J. Viral. Hepat. 7:2-14.
29. Ménez R, et al. 2003. Crystal structure of a hydrophobic immunodominant antigenic site on hepatitis C virus core protein complexed to monoclonal antibody 19D9D6. J. Immunol. 170:1917-1924.
30. Miyanari Y, et al. 2007. The lipid droplet is an important organelle for hepatitis C virus production. Nat. Cell Biol. 9:1089-1097.
31. Moradpour D, Penin F, Rice CM. 2007. Replication of hepatitis C virus. Nat. Rev. Microbiol. 5:453-463.
32. Murray CL, Jones CT, Tassello J, Rice CM. 2007. Alanine scanning of the hepatitis C virus core protein reveals numerous residues essential for production of infectious virus. J. Virol. 81:10220-10231.
33. Rice LM, Brunger AT. 1994. Torsion angle dynamics: reduced variable conformational sampling enhances crystallographic structure refinement. Proteins 19:277-290.
34. Robertson B, et al. 1998. Classification, nomenclature, and database development for hepatitis C virus (HCV) and related viruses: proposals for standardization. International Committee on Virus Taxonomy. Arch. Virol. 143:2493-2503.
35. Santolini E, Migliaccio G, La Monica N. 1994. Biosynthesis and biochemical properties of the hepatitis C virus core protein. J. Virol. 68: 3631-3641.
36. Schwieters CD, Kuszewski JJ, Tjandra N, Clore GM. 2003. The Xplor-NIH NMR molecular structure determination package. J. Magn. Reson. 160:65-73.
37. Shavinskaya A, Boulant S, Penin F, McLauchlan J, Bartenschlager R. 2007. The lipid droplet binding domain of hepatitis C virus core protein is a major determinant for efficient virus assembly. J. Biol. Chem. 282: 37158-37169.
38. Shepard CW, Finelli L, Alter MJ. 2005. Global epidemiology of hepatitis C virus infection. Lancet Infect. Dis. 5:558-567.
39. Shimoike T, Mimori S, Tani H, Matsuura Y, Miyamura T. 1999. Interaction of hepatitis C virus core protein with viral sense RNA and suppression of its translation. J. Virol. 73:9718-9725.
40. Simmonds P, et al. 2005. Consensus proposals for a unified system of nomenclature of hepatitis C virus genotypes. Hepatology 42:962-973.
41. Soriano V, Peters MG, Zeuzem S. 2009. New therapies for hepatitis C virus infection. Clin. Infect. Dis. 48:313-320.
42. Stein EG, Rice LM, Brunger AT. 1997. Torsion-angle molecular dynamics as a new efficient tool for NMR structure calculation. J. Magn. Reson. 124:154-164.
43. Vieyres G, et al. 2010. Characterization of the envelope glycoproteins associated with infectious hepatitis C virus. J. Virol. 84:10159-10168.
44. Wakita T, et al. 2005. Production of infectious hepatitis C virus in tissue culture from a cloned viral genome. Nat. Med. 11:791-796.
45. Witteveldt J, et al. 2009. CD81 is dispensable for hepatitis C virus cellto-cell transmission in hepatoma cells. J. Gen. Virol. 90:48-58.