Selective precipitation and purification of monovalent proteins using oligovalent ligands and ammonium sulfate

Bioconjugate Chemistry

Volumn 23, Issue 2, 2012, Pages 293-299

  Mirica, Katherine A ^a   Lockett, Matthew R ^a   Snyder, Phillip W ^a   Shapiro, Nathan D ^a   MacK, Eric T ^a   Nam, Sarah ^a   Whitesides, George M ^a,b  

^a HARVARD UNIVERSITY   (United States)

^b HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AGGREGATES; BIOSYNTHESIS; NITROGEN COMPOUNDS; PRECIPITATION (CHEMICAL); PURIFICATION; REMOVAL; SULFUR COMPOUNDS;

AGGREGATE FORMATION; CELL LYSATES; CELLULAR LYSATES; COMPLEX PRECIPITATE; HIGH MOLECULAR WEIGHT; HUMAN CARBONIC ANHYDRASES; PROTEIN LIGANDS; SELECTIVE PRECIPITATION; SELECTIVE PURIFICATION; TARGET PROTEINS;

LIGANDS;

AMMONIUM SULFATE; CARBONATE DEHYDRATASE; LIGAND; SODIUM CHLORIDE; SULFONAMIDE;

ARTICLE; CELL LYSATE; CHROMATOGRAPHY; CONTROLLED STUDY; DIALYSIS; HUMAN; NONHUMAN; PRECIPITATION; PROTEIN PURIFICATION; SOLUBILITY;

EID: 84856910946     PISSN: 10431802     EISSN: 15204812     Source Type: Journal    
DOI: 10.1021/bc200390q     Document Type: Article

References (17)

1. Arakawa, T. and Timasheff, S. N. (1985) Theory of protein solubility Methods Enzymol. 114, 49-77
2. Chiew, Y. C., Kuehner, D., Blanch, H. W., and Prausnitz, J. M. (1995) Molecular thermodynamics for salt-induced protein precipitation AIChE J. 41, 2150-2159
3. Hatti-Kaul, R. and Mattiasson, B. (2003) Isolation and purification of proteins, Marcel Dekker, New York.
4. Wheelwright, S. M. (1991) Protein purification: design and scale up of downstream processing, Hanser Publishers; Distributed in the U.S.A. and in Canada by Oxford University Press, New York.
5. Scopes, R. K. (1994) Protein purification: principles and practice, Springer-Verlag, New York.
6. Bilgicer, B., Thomas, S. W., Shaw, B. F., Kaufman, G. K., Krishnamurthy, V. M., Estroff, L. A., Yang, J., and Whitesides, G. M. (2009) A non-chromatographic method for the purification of a bivalently active monoclonal IgG antibody from biological fluids J. Am. Chem. Soc. 131, 9361-9367
7. Flygare, S., Griffin, T., Larsson, P. O., and Mosbach, K. (1983) Affinity precipitation of dehydrogenases Anal. Biochem. 133, 409-416
8. Gupta, M. N., Kaul, R., Guoqiang, D., Dissing, U., and Mattiasson, B. (1996) Affinity precipitation of proteins J. Mol. Recognit. 9, 356-359 (Pubitemid 126446979)
9. Hilbrig, F. and Freitag, R. (2003) Protein purification by affinity precipitation J. Chromatogr., B: Anal. Technol. Biomed. Life Sci. 790, 79-90 (Pubitemid 36588772)
10. Mammen, M., Choi, S. K., and Whitesides, G. M. (1998) Polyvalent interactions in biological systems: implications for design and use of multivalent ligands and inhibitors Angew. Chem., Int. Ed. 37, 2754-2794 (Pubitemid 29008561)
11. Krishnamurthy, V. M., Kaufman, G. K., Urbach, A. R., Gitlin, I., Gudiksen, K. L., Weibel, D. B., and Whitesides, G. M. (2008) Carbonic anhydrase as a model for biophysical and physical-organic studies of proteins and protein-ligand binding Chem. Rev. 108, 946-1051 (Pubitemid 351577855)
12. Mack, E. T., Snyder, P. W., Perez-Castillejos, R., and Whitesides, G. M. (2011) Using covalent dimers of human carbonic anhydrase ii to model bivalency in immunoglobulins J. Am. Chem. Soc. 133, 11701-11715
13. Jain, A., Huang, S. G., and Whitesides, G. M. (1994) Lack of effect of the length of oligoglycine-derived and oligo(ethylene-glycol)-derived para-substituents on the affinity of benzenesulfonamides for carbonic-anhydrase-II in solution J. Am. Chem. Soc. 116, 5057-5062 (Pubitemid 24241755)
14. Krishnamurthy, V. M., Semetey, V., Bracher, P. J., Shen, N., and Whitesides, G. M. (2007) Dependence of effective molarity on linker length for an intramolecular protein-ligand system J. Am. Chem. Soc. 129, 1312-1320 (Pubitemid 46208603)
15. Wang, Z.-X. (1995) An exact mathematical expression for describing competitive binding of two different ligands to a protein molecule FEBS Lett. 360, 111-114
16. Mack, E. T., Perez-Castillejos, R., Suo, Z., and Whitesides, G. M. (2008) Exact analysis of ligand-induced dimerization of monomeric receptors Anal. Chem. 80, 5550-5555
17. Nair, S. K., Calderone, T. L., Christianson, D. W., and Fierke, C. A. (1991) Altering the mouth of the hydrophobic pocket. structure and kinetics of human carbonic anhydrase II mutants at residue Val-121 J. Biol. Chem. 266, 17320-17325 (Pubitemid 21907942)