A non-chromatographic method for the purification of a bivalently active monoclonal IgG antibody from biological fluids

Journal of the American Chemical Society

Volumn 131, Issue 26, 2009, Pages 9361-9367

  Bilgiçer, Başar ^a   Thomas III, Samuel W ^a   Shaw, Bryan F ^a   Kaufman, George K ^a   Krishnamurthy, Vijay M ^a   Estroff, Lara A ^a   Yang, Jerry ^a   Whitesides, George M ^a  

^a HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIUM SULFATE; BINDING CONSTANT; BIOLOGICAL FLUIDS; CHROMATOGRAPHIC METHODS; CHROMATOGRAPHIC SEPARATIONS; DINITROPHENYL; MULTIVALENT LIGANDS; SELECTIVE PRECIPITATION;

AMMONIUM COMPOUNDS; ANTIGENS; BINDING ENERGY; BODY FLUIDS; FLUIDS; LIQUID CHROMATOGRAPHY; MONOCLONAL ANTIBODIES; OLIGOMERS; PROTEINS; PURIFICATION; SPIN POLARIZATION;

BINDING SITES;

2,4 DINITROPHENOL; AMMONIUM SULFATE; DIGOXIN; DIMER; GLOBULIN; HAPTEN; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN G ANTIBODY; OLIGOMER;

ARTICLE; ASCITES FLUID; BINDING AFFINITY; BINDING SITE; GEL PERMEATION CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION PROCEDURE; MOUSE; NONHUMAN; PRECIPITATION; PURIFICATION; RAT;

AMMONIUM SULFATE; ANIMALS; ANTIBODIES, MONOCLONAL; ASCITES; BINDING SITES, ANTIBODY; CHROMATOGRAPHY, GEL; DIGOXIN; DIMERIZATION; DINITROBENZENES; HAPTENS; IMMUNOGLOBULIN G; MICE; MODELS, MOLECULAR; PRECIPITATION; RATS;

EID: 67649980003     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9023836     Document Type: Article

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