Purification, molecular characterization and gene expression analysis of an aspartic protease (Sc-ASP113) from the nematode Steinernema carpocapsae during the parasitic stage

Molecular and Biochemical Parasitology

Volumn 182, Issue 1-2, 2012, Pages 37-44

  Balasubramanian, Natesan ^a   Toubarro, Duarte ^a   Nascimento, Gisela ^a   Ferreira, Ricardo ^a   Simões, Nelson ^a  

^a UNIVERSITY OF THE AZORES   (Portugal)

Author keywords

Aspartic protease; Expressed sequence tag; Gene expression; Parasitic stage; Steinernema carpocapsae

Indexed keywords

ASPARTIC PROTEINASE; COMPLEMENTARY DNA; HEMOGLOBIN; SIGNAL PEPTIDE;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SUBSTRATE; EXPRESSED SEQUENCE TAG; GENE DOSAGE; GENE EXPRESSION; GENE STRUCTURE; HAEMONCHUS CONTORTUS; IN SITU HYBRIDIZATION; MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR WEIGHT; NEMATODE; NEMATODE LIFE CYCLE STAGE; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE DEVELOPMENT; PHYLOGENETIC TREE; PRIORITY JOURNAL; PROTEIN DOMAIN; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS; SOUTHERN BLOTTING; STEINERNEMA CARPOCAPSAE; UPREGULATION;

ANIMALS; ASPARTIC ACID PROTEASES; CATALYTIC DOMAIN; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DNA, COMPLEMENTARY; EXPRESSED SEQUENCE TAGS; FEMALE; GENE EXPRESSION REGULATION; GENES, HELMINTH; HELMINTH PROTEINS; HEMOGLOBINS; HOST-PARASITE INTERACTIONS; INSECTS; MALE; MASS SPECTROMETRY; NEMATODA; NEMATODE INFECTIONS; PHYLOGENY; PROTEIN SORTING SIGNALS; TIME FACTORS; UP-REGULATION;

BACTERIA (MICROORGANISMS); HAEMONCHUS CONTORTUS; HEXAPODA; NEMATODA; STEINERNEMA CARPOCAPSAE; STEINERNEMA FELTIAE; XENORHABDUS NEMATOPHILA;

EID: 84856716427     PISSN: 01666851     EISSN: 18729428     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2011.12.001     Document Type: Article

References (34)

1. R.U. Ehlers Mass production of entomopathogenic nematodes for plant protection Appl Microbiol Biotechnol 56 2001 623 633
2. D.I. Shapiro-Ilan, E.E. Lewis, Y. Son, and W.L. Tedders Superior efficacy observed in entomopathogenic nematodes applied in infected-host cadavers compared with application in aqueous suspension J Invertebr Pathol 83 2003 270 272 (Pubitemid 36953232)
3. M. Sicard, K. Brugirard-Ricaud, S. Pages, A. Lanois, N.E. Boemare, and M. Brehelin Stages of infection during the tripartite interaction between Xenorhabdus nematophila, its nematode vector, and insect hosts Appl Environ Microbiol 70 2004 6473 6480 (Pubitemid 39518553)
4. J.M. Dzik Molecules released by helminth parasites involved in host colonization Acta Biochim Pol 53 1 2006 33 64
5. D. Toubarro, M. Lucena-Robles, G. Nascimento, R. Santos, R. Montiel, and P. Veríssimo Serine protease-mediated host invasion by the parasitic nematode Steinernema carpocapsae J Biol Chem 285 2010 30666 30675
6. S. Forst, B. Dowds, N. Boemare, and E. Stackebrandt Xenorhabdus and Photorhabdus spp. bugs that kill bugs Annu Rev Microbiol 51 1997 47 72 (Pubitemid 27433042)
7. Y.J. Jing, D. Toubarro, Y.J. Hao, and N. Simões Cloning, characterisation and heterologous expression of an astacin metalloprotease, Sc-AST, from the entomoparasitic nematode Steinernema carpocapsae Mol Biochem Parasitol 174 2010 101 108
8. Y. Hao, R. Montiel, S. Abubucker, M. Mitreva, and N. Simões Transcripts analysis of the entomopathogenic nematode Steinernema carpocapsae induced in vitro with insect haemolymph Mol Biochem Parasitol 169 2 2010 79 86
9. S. Kocabiyik, and H. Ozel An extracellular-pepstatin insensitive acid protease produced by Thermoplasma volcanium Bioresour Technol 98 2007 112 117 (Pubitemid 44301897)
10. P.J. Brindley, B.H. Kalinna, J.Y. Wong, B.J. Bogitsh, L.T. King, and D.J. Smyth Proteolysis of human hemoglobin by Schistosome cathepsin D Mol Biochem Parasitol 112 2001 103 112 (Pubitemid 32144841)
11. A. Brown, N. Girod, E.E. Billett, and D.I. Pritchard Necator americanus (human hookworm) aspartyl proteinases and digestion of skin macromolecules during skin penetration Am J Trop Med Hyg 60 1999 840 847 (Pubitemid 29233091)
12. A. Jolodar, P. Fischer, D.W. Buttner, D.J. Miller, C. Schmetz, and N.W. Brattig Onchocerca volvulus: expression and immunolocalization of a nematode cathepsin D-like lysosomal aspartic protease Exp Parasitol 107 2004 145 156 (Pubitemid 39194166)
13. D. Longbottom, D.L. Redmond, M. Russell, S. Liddell, W.D. Smith, and D.P. Knox Molecular cloning and characterisation of a putative aspartate proteinase associated with a gut membrane protein complex from adult Haemonchus contortus Mol Biochem Parasitol 88 1997 63 72 (Pubitemid 27346818)
14. R.A. Bedding, A.S. Molyneux, and R.J. Akhurst Heterorhabditis spp., Neoaplectana spp., and Steinernema kraussei: interspecific and intraspecific differences in infectivity for insects Exp Parasitol 55 1983 249 257
15. N. Balasubramanian, Y.J. Hao, D. Toubarro, G. Nascimento, and N. Simões Purification, biochemical and molecular analysis of a chymotrypsin protease with prophenoloxidase suppression activity from the entomopathogenic nematode Steinernema carpocapsae Int J Parasitol 39 2009 975 984
16. N. Balasubramanian, D. Toubarro, and N. Simoes Biochemical study and in vitro insect immune suppression by a trypsin-like secreted protease from the nematode Steinernema carpocapsae Parasite Immunol 32 2010 165 175
17. S.R. Dutky Insect microbiology Adv Appl Microbiol 1 1959 175 200
18. H. Xiao, T. Tanaka, M. Ogawa, and R.Y. Yada Expression and enzymatic characterization of the soluble recombinant plasmepsin I from Plasmodium falciparum Protein Eng Des Sel 10 2007 1 9
19. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
20. J. Gobom, E. Nordhoff, E. Mirgorodskaya, R. Ekman, and P. Roepstorff Sample purification and preparation technique based on nano-scale reversed-phase columns for the sensitive analysis of complex peptide mixtures by matrix assisted laser desorption/ionization mass spectrometry J Mass Spectrom 34 1999 105 116
21. R. Santos, G. da Costa, C. Franco, P. Gomes-Alves, P. Flammang, and A.V. Coelho First insights into the biochemistry of tube foot adhesive from the sea urchin Paracentrotus lividus (Echinoidea, Echinodermata) Mar Biotechnol 11 6 2009 686 698
22. K.J. Livak, and T.D. Schmittgen Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta DeltaC(T)) method Methods 25 2001 402 408 (Pubitemid 34164012)
23. J.M. de Boer, Y. Yan, E.L. Davis, G. Smant, and T.J. Baum In-situ hybridization to messenger RNA in Heterodera glycines J Nematol 30 1998 309 312
24. P.B. Szecsi The aspartic proteases Scand J Clin Lab Invest Suppl 210 1992 5 22
25. L.V. Mello, H.O. Meara, D.J. Rigden, and S. Paterson Identification of novel aspartic proteases from Strongyloides ratti and characterisation of their evolutionary relationships, stage-specific expression and molecular structure BMC Genomics 10 2009 611 625
26. L. Jean, M. Long, J. Young, P. Pery, and F. Tomley Aspartyl proteinase genes from picomplexan parasites: evidence for evolution of the gene structure Trends Parasitol 17 2001 491 498 (Pubitemid 32918269)
27. M.E. Morales, H. Bernd, Kalinnac, O. Heyersc, H. Victoria, and Manna Genomic organization of the Schistosoma mansoni aspartic protease gene, a platyhelminth orthologue of mammalian lysosomal cathepsin D Gene 338 2004 99 109 (Pubitemid 39092416)
28. A.A. Hill, C.P. Hunter, B.T. Tsung, G. Tucker-Kellogg, and E.L. Brown Genomic analysis of gene expression in C. elegans Science 290 5492 2000 809 812
29. B.J. Bogitsh, and K.F. Kirschner Schistosoma japonicum: immunocytochemistry of adults using heterologous antiserum to bovine cathepsin D Exp Parasitol 64 2 1987 213 218
30. Y.J. Hao, R. Montiel, G. Nascimento, D. Toubarro, and N. Simoes Identification and expression analysis of the Steinernema carpocapsae elastase-like serine protease gene during the parasitic stage Exp Parasitol 122 2009 51 60
31. R.S. Hussey Disease-inducing secretions of plant-parasitic nematodes Annu Rev Phytopathol 27 1989 123 141
32. A. Jolodar Determination of aspartic protease gene dosage in the Onchocerca volvulus genome Iran J Vet Res 11 4 2010 368 372
33. M.M. Becker, S.A. Harrop, J.P. Dalton, B.H. Kalinna, D.P. McManus, and B.J. Brindley Cloning and characterization of the Schistosoma japonicum aspartic proteinase involved in hemoglobin degradation J Biol Chem 270 41 1995 24496 24501
34. F. Petry, M.W. Shirley, M.A. Miles, and V. McDonald Characterisation of a Cryptosporidium parvum-specific cDNA clone and detection of parasite DNA in mucosal scrapings of infected mice Mol Biochem Parasitol 95 1 1998 21 31 (Pubitemid 28404735)