Necator americanus (human hookworm) aspartyl proteinases and digestion of skin macromolecules during skin penetration

American Journal of Tropical Medicine and Hygiene

Volumn 60, Issue 5, 1999, Pages 840-847

  Brown, Alan ^a   Girod, Nadine ^b   Billett, Ellen E ^b   Pritchard, David I ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^b NOTTINGHAM TRENT UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC PROTEINASE; COLLAGEN TYPE 1; COLLAGEN TYPE 3; COLLAGEN TYPE 5; ELASTIN; FIBRONECTIN; LAMININ; METALLOPROTEINASE; PEPSTATIN; SERINE PROTEINASE;

ARTICLE; COLLAGEN DEGRADATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; HAMSTER; LARVA; MACROMOLECULE; NECATOR AMERICANUS; NONHUMAN; SKIN PENETRATION;

EID: 0032900715     PISSN: 00029637     EISSN: None     Source Type: Journal    
DOI: 10.4269/ajtmh.1999.60.840     Document Type: Article

References (26)

1. Matthews BE, 1982. Skin penetration by Necator americanus larvae. Z Parasitenkd 68: 81-86.
2. Salafsky B, Fusco AC, Siddiqui A, 1990. Necator americanus: factors influencing skin penetration by larvae. Schad GA, Warren KS, eds. Hookworm Disease: Current Status and New Directions. London: Taylor and Francis, 326-329.
3. Kumar S, Pritchard DI, 1992. The partial characterisation of proteases present in the excretory/secretory products and exsheathing fluid of the infective (L3) larva of Necator americanus. Int J Parasitol 22: 563-572.
4. Kumar S, Pritchard DI, 1992. Secretion of metalloproteases by living infective larvae of Necator americanus. J Parasitol 78: 917-919.
5. Sen HG, Seth D, 1967. Complete development of the human hookworm, Necator americanus, in golden hamsters, Mesocricetus auratus. Nature 214: 609-610.
6. Harada Y, Mori O, 1955. A new method of culturing hookworms. Y Acta Med 1: 177-179.
7. Hawdon JM, Schad GA, 1991. Long term storage of hookworm infective larvae in buffered saline solution maintains larval responsiveness to host signals. J Helminthol Soc (Wash) 58: 140-142.
8. Bradford MM, 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248-254.
9. Beynon RJ, Bond JS, 1989. Proteolytic Enzymes - A Practical Approach. Oxford, United Kingdom: IRL Press.
10. Lottenberg R, Christensen U, Jackson CM, Coleman PL, 1981. Assay of coagulation proteases using peptide chromogenic and fluorogenic substrates. Methods Enzymol 80: 341-361.
11. Graf L, Cralk CS, Patthy A, Roczniak S, Fletterick RJ, Rutter WJ, 1987. Selective alteration of substrate specificity by replacement of aspartic acid with lysine in the binding pocket of trypsin. Biochemistry 26: 2616-2623.
12. Ishura S, Sano M, Kamakura K, Sugita H, 1985. Isolation of two forms of the high molecular mass serine protease, ingensin, from porcine skeletal muscle. FEBS Lett 198: 119-123.
13. Hardy MF, Mantle D, Pennington RJT, 1983. Characteristics of a new muscle proteinase. Biochem Soc Trans 11: 348-349.
14. Barrett AJ, 1980. Fluorometric assays for cathepsin B and cathepsin H with methylcoumarylamide substrates. Biochem J 187: 909-912.
15. Morita T, Kata H, Iwanaga S, Takada K, Kimura T, Sakakibara S, 1977. New fluorogenic substrates for a-thrombin, factor Xa, kallikreins, and urokinase. J Biochem 82: 1495-1498.
16. Zimmerman M, Ashe BM, Yurewicz EC, Patel G, 1977. Sensitive assays for trypsin, elastase and chymotrypsin using new fluorogenic substrates. Anal Biochem 78: 47-51.
17. Dowd AJ, Dalton JP, Loukas AC, Prociv P, Brindley PJ, 1994. Secretion of cysteine proteinase by the zoonotic hookworm Ancylostoma caninum. Am J Trop Med Hyg 51: 341-347.
18. Knox DP, Kennedy MW, 1988. Proteinases released by the parasitic larval stages of Ascaris suum and their inhibition by antibody. Mol Biochem Parasitol 28: 207-216.
19. Laemmli UK, 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
20. Towbin H, Staehelin T, Gordon J, 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications Proc Natl Acad. Sci USA 76: 4350-4354.
21. Kumar S, Pritchard DI, 1992. Skin penetration by ensheathed third-stage infective larvae of N. americanus, and the host's immune response to larval antigens. Int. J Parasitol 22: 573-579.
22. Brown A, Burleigh, Billett EE, Pritchard DI, 1995. An initial characterisation of the proteolytic enzymes secreted by the adult stage of the human hookworm Necator americanus. Parasitology 110: 555-563.
23. Hotez P, Haggerty J, Hawdon J, Milstone L, Gamble HR, Schad G, Richards F, 1990. Metalloproteinases of infective Ancylostoma hookworm larvae and their possible functions in tissue invasion and ecdysis. Infect Immun 58: 3883-3892.
24. Karanu FN, Rurangirwa FR, McGuire TC, Jasmer DP, 1993. Haemonchus contortus: identification of proteases with diverse characteristics in adult worm excretory-secretory products. Exp Parasitol 77: 362-371.
25. Harrap SA, Prociv P, Brindley PJ, 1996. ACASP, A gene encoding a cathepsin D-like protease from the hookworm Ancylostoma caninum. Biochem Biophys Res Comm 227: 294-302.
26. McKerrow JH, Brindley P, Brown M, Gam AA, Staunton C, Neva F, 1990. Strongyloides stercoralis: identification of a protease that facilitates penetration of skin by infective larvae. Exp Parasitol 70: 134-143.