Expression of biologically active human clotting factor IX in Drosophila S2 cells: γ-carboxylation of a human vitamin K-dependent protein by the insect enzyme

Biotechnology Progress

Volumn 28, Issue 1, 2012, Pages 45-51

  Vatandoost, Jafar ^a,b   Zomorodipour, Alireza ^b   Sadeghizadeh, Majid ^a   Aliyari, Roghayeh ^c   Bos, Mettine H A ^d   Ataei, Fariba ^b  

^a TARBIAT MODARES UNIVERSITY   (Iran)

^b NATIONAL INSTITUTE OF GENETIC ENGINEERING AND BIOTECHNOLOGY   (Iran)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

carboxylation; Coagulation factor IX; Drosophila S2 cell line; Propeptide

Indexed keywords

CELL LINES; CHINESE HAMSTER OVARY CELLS; CO-EXPRESSION; COAGULATION FACTOR; COAGULATION FACTOR IX; COPPER IONS; CULTURE SUPERNATANT; DROSOPHILA CELLS; DROSOPHILA S2 CELL LINE; EXPRESSION EFFICIENCY; EXPRESSION LEVELS; HUMAN ENZYMES; IN-VITRO; IN-VIVO; METALLOTHIONEINS; PRODUCT YIELDS; PROPEPTIDES; SUBSTRATE RECOGNITION;

BARIUM; BIOSYNTHESIS; CARBOXYLATION; CELLS; COAGULATION; ENZYMES; METAL IONS; PROTEINS; SUBSTRATES;

CELL CULTURE;

BLOOD CLOTTING FACTOR 9; GLUTAMYL CARBOXYLASE; LIGASE; RECOMBINANT PROTEIN; VITAMIN K GROUP;

ANIMAL; ARTICLE; BIOSYNTHESIS; BLOOD CLOTTING; CHO CELL; CYTOLOGY; DROSOPHILA; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; GENETICS; HAMSTER; HUMAN; METABOLISM; PLASMID; PROTEIN PROCESSING;

ANIMALS; BLOOD COAGULATION; CARBON-CARBON LIGASES; CHO CELLS; CRICETINAE; DROSOPHILA; FACTOR IX; GENE EXPRESSION REGULATION; HUMANS; PLASMIDS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; TRANSFECTION; VITAMIN K;

CRICETULUS GRISEUS; HEXAPODA; VERTEBRATA;

EID: 84856578571     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.723     Document Type: Article

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