Controlled release of nutrients to mammalian cells cultured in shake flasks

Biotechnology Progress

Volumn 28, Issue 1, 2012, Pages 188-195

  Hegde, Swati ^a,b   Pant, Tejal ^a,c   Pradhan, Ketaki ^a   Badiger, Manohar ^a   Gadgil, Mugdha ^a  

^a NATIONAL CHEMICAL LABORATORY   (India)

^b NATIONAL INSTITUTE OF TECHNOLOGY KARNATAKA   (India)

^c Hislop School of Biotechnology   (India)

Author keywords

[No Author keywords available]

Indexed keywords

BATCH MODES; CELL LINES; CHINESE HAMSTER OVARY; CONCENTRATED FEED; CONTINUOUS FEEDING; CONTROLLED RELEASE; INITIAL PROCESS; LACTATE CONCENTRATION; LOW LEVEL; MAMMALIAN CELLS; METABOLITE PRODUCTION; NUTRIENT CONCENTRATIONS; NUTRIENT DELIVERY; PROTEIN HYDROLYSATE; PROTEIN PRODUCTION; SCALE DOWN; SCALE DOWN MODEL; SHAKE FLASKS; VIABLE CELLS;

ANIMAL CELL CULTURE; FEEDING; GLUCOSE; HYDROGELS;

NUTRIENTS;

GLUCOSE; LACTIC ACID;

ANIMAL; ARTICLE; BIOREACTOR; CELL COUNT; CHEMISTRY; CHO CELL; CULTURE MEDIUM; CULTURE TECHNIQUE; FEMALE; FOOD; HAMSTER; HYDROGEL; KINETICS; METABOLISM; METHODOLOGY;

ANIMALS; BIOREACTORS; CELL COUNT; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CULTURE MEDIA; FEMALE; FOOD; GLUCOSE; HYDROGELS; KINETICS; LACTIC ACID;

CRICETULUS GRISEUS; MAMMALIA;

EID: 84856559861     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.729     Document Type: Article

References (34)

1. Gadgil M, Carrier T. Increasing performance of mammalian expression platforms. In: Langer E, editor. Advances in Large-Scale Biopharmaceutical Manufacturing and Scale-Up Production, 2nd ed. Bioplan Associates and ASM Press; 2007.
2. Hu W-S, Aunins JG. Large-scale mammalian cell culture. Curr Opin Biotechnol. 1997; 8: 148-153.
3. Wlaschin KF, Hu W-S. Fedbatch culture and dynamic nutrient feeding. Adv Biochem Eng Biotechnol. Springer: Berlin, Heidelberg, 2006; 101: 43-74.
4. van Berkel PHC, Gerritsen J, Perdok G, Valbjørn J, Vink T, van de Winkel JGJ, Parren PWHI. N-linked glycosylation is an important parameter for optimal selection of cell lines producing biopharmaceutical human IgG. Biotechnol Prog. 2009; 25: 244-251.
5. Silk N, Denby S, Lewis G, Kuiper M, Hatton D, Field R, Baganz F, Lye G. Fed-batch operation of an industrial cell culture process in shaken microwells. Biotechnol Lett. 2010; 32: 73-78.
6. Lindgren K, Salmén A, Lundgren M, Bylund L, Ebler Å, Fäldt E, Sörvik L, Fenge C, Skoging-Nyberg U. Automation of cell line development. Cytotechnology 2009; 59: 1-10.
7. Marques MPC, Cabral JMS, Fernandes P. High throughput in biotechnology: from shake-flasks to fully instrumented microfermentors. Rec Pat Biotechnol. 2009; 3: 124-140.
8. Legmann R, Schreyer HB, Combs RG, McCormick EL, Russo AP, Rodgers ST. A predictive high-throughput scale-down model of monoclonal antibody production in CHO cells. Biotechnol Bioeng. 2009; 104: 1107-120.
9. Stokelman A, Slaff G, Zazur A, Rodgers F, Low D, Seewoester T, High throughput cell culture experimentation with Bioprocessors' SimCell Platform. In: Biochemical Engineering XIV: Frontiers and Advances in Biotechnology, Biological and Biomolecular Engineering. British Columbia, Canada; July 10-14, 2005.
10. Thomas D, Ward T, Drake R, Hollway R, Savage D, Owen S. A novel automated approach to enabling high-throughput cell line development, selection, and other cell culture tasks performed in Erlenmeyer (shake) flasks. J Assoc Lab Autom. 2008; 13: 145-151.
11. Gryseels T. Considering cell culture automation in upstream bioprocess development. Bioproc Intl. 2008; 6: 12-16.
12. Büchs J. Introduction to advantages and problems of shaken cultures. Biochem Eng J. 2001; 7: 91-98.
13. Jänicke G, Sauter C, Bux R, Haas J. Characterisation of shake flasks for cultivation of animal cell cultures. In: Smith R, editor. Cell Technology for Cell Products, Proceedings of the 19th ESACT Meeting, Harrogate, UK, June 5-8, 2005. The Netherlands: Springer; 2007.
14. Zhou WC, Rehm J, Hu WS. High viable cell concentration fed-batch cultures of hybridoma cells through online nutrient feeding. Biotechnol Bioeng. 1995; 46: 579-587.
15. Chee, FWD, Wong KTW, Goh LT, Heng CK, Yap MGS. Impact of dynamic online fed-batch strategies on metabolism, productivity and N-glycosylation quality in CHO cell cultures. Biotechnol Bioeng. 2005; 89: 164-177.
16. Yuk IH, Zhang B, Yang Y, Dutina G, Leach KD, Vijayasankaran N, Shen AY, Andersen DC, Snedecor BR, Joly JC. Controlling glycation of recombinant antibody in fed-batch cell cultures. Biotechnol Bioeng. 2011; 108: 2600-2610.
17. Maharbiz MM, Holtz WJ, Howe RT, Keasling JD. Microbioreactor arrays with parametric control for high-throughput experimentation. Biotechnol Bioeng. 2004; 85: 376-381.
18. Puskeiler R, Kaufmann K, Weuster-Botz D. Development, parallelization, and automation of a gas-inducing milliliter-scale bioreactor for high-throughput bioprocess design (HTBD). Biotechnol Bioeng. 2005; 89: 512-523.
19. Weuster-Botz D, Parallel reactor systems for bioprocess development. Adv Biochem Eng Biotechnol. 2005; 92: 125-143.
20. Weuster-Botz D, Altenbach-Rehm J, Arnold M. Parallel substrate feeding and pH-control in shaking-flasks. Biochem Eng J 2001; 7: 163-170.
21. Panula-Perälä J, Šiurkus J, Vasala A, Wilmanowski R, Casteleijn MG, Neubauer P. Enzyme controlled glucose auto-delivery for high cell density cultivations in microplates and shake flasks. Microb Cell Fact. 2008; 7:31.
22. Künzler JF, Hydrogels. In Encyclopedia of Polymer Science and Technology. John Wiley & Sons, Inc.; 2002. Available at http://onlinelibrary.wiley.com/.
23. Hoare TR, Kohane DS. Hydrogels in drug delivery: progress and challenges. Polymer 2008; 49: 1993-2007.
24. Hoffman AS. Hydrogels for biomedical applications. Adv Drug Deliv Rev. 2002; 54: 3-12.
25. Lee KY, Mooney DJ. Hydrogels for tissue engineering. Chem Rev. 2001; 101: 1869-1880.
26. Silva AKA, Richard C, Bessodes M, Scherman D, Merten O-W. Growth factor delivery approaches in hydrogels. Biomacromolecules 2008; 10: 9-18.
27. Jeude M, Dittrich B, Niederschulte H, Anderlei T, Knocke C, Klee D, Büchs J. Fed-batch mode in shake flasks by slow-release technique. Biotechnol Bioeng. 2006; 95: 433-445.
28. Scheidle M, Jeude M, Dittrich B, Denter S, Kensy F, Suckow M, Klee D, Büchs J. High-throughput screening of Hansenula polymorpha clones in the batch compared with the controlled-release fed-batch mode on a small scale. FEMS Yeast Res. 2010; 10: 83-92.
29. De Jesus MJ, Girard P, Bourgeois M, Baumgartner G, Jacko B, Amstutz H, Wurm FM. TubeSpin satellites: a fast track approach for process development with animal cells using shaking technology. Biochem Eng J. 2004; 17: 217-223.
30. Zhou WC, Rehm J, Europa AF, Hu W-S. Alteration of mammalian cell metabolism by dynamic nutrient feeding. Cytotechnology 1997; 24: 99-108.
31. Refojo MF, Yasuda H. Hydrogels from 2-hydroxyethyl methacrylate and propylene glycol monoacrylate. J Appl Polym Sci. 1965; 9: 2425-2435.
32. Wichterle O, Lim D. Hydrophilic gels for biological use. Nature 1960; 185: 117-118.
33. Canal T, Peppas NA. Correlation between mesh size and equilibrium degree of swelling of polymeric networks. J Biomed Mater Res. 1989; 23: 1183-1193.
34. Peppas NA, Moynihan HJ, Lucht LM. The structure of highly crosslinked poly(2-hydroxyethyl methacrylate) hydrogels. J Biomed Mater Res. 1985; 19: 397-411.