A dual approach employing MALDI-TOF MS and real-time PCR for fast species identification within the Enterobacter cloacae complex

FEMS Microbiology Letters

Volumn 328, Issue 1, 2012, Pages 46-53

  Pavlovic, Melanie ^a   Konrad, Regina ^a   Iwobi, Azuka N ^a   Sing, Andreas ^a   Busch, Ulrich ^a   Huber, Ingrid ^a  

^a Bavarian Health and Food Safety Authority   (Germany)

Author keywords

E. cloacae complex; Enterobacter cloacae; MALDI TOF MS; Real time PCR

Indexed keywords

ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; COMPARATIVE GENOMIC HYBRIDIZATION; CONTROLLED STUDY; ENTEROBACTER; ENTEROBACTER ASBURIAE; ENTEROBACTER CLOACAE; ENTEROBACTER HORMAECHEI; ENTEROBACTER KOBEI; ENTEROBACTER LUDWIGII; LIMIT OF DETECTION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR DYNAMICS; MULTILOCUS SEQUENCE TYPING; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; REAL TIME POLYMERASE CHAIN REACTION;

BACTERIOLOGICAL TECHNIQUES; ENTEROBACTER CLOACAE; ENTEROBACTERIACEAE INFECTIONS; HSP40 HEAT-SHOCK PROTEINS; HUMANS; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ENTEROBACTER; ENTEROBACTER ASBURIAE; ENTEROBACTER CLOACAE; ENTEROBACTER CLOACAE COMPLEX; ENTEROBACTER HORMAECHEI; ENTEROBACTER KOBEI; ENTEROBACTER LUDWIGII;

EID: 84856486113     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2011.02479.x     Document Type: Article

References (18)

1. Anderson A, Pietsch K, Zucker R, Mayr A, Müller-Hohe E, Messelhäusser U, Sing A, Busch U & Huber I (2011) Validation of a duplex real-time PCR for the detection of Salmonella spp. in different food products. Food Anal Methods 4: 259-267.
2. van Baar BL (2000) Characterisation of bacteria by matrix-assisted laser desorption/ionisation and electrospray mass spectrometry. FEMS Microbiol Rev 24: 193-219.
3. Brenner DJ, McWhorter AC, Kai A, Steigerwalt AG & Farmer JJ III (1986) Enterobacter asburiae sp. nov., a new species found in clinical specimens, and reassignment of Erwinia dissolvens and Erwinia nimipressuralis to the genus Enterobacter as Enterobacter dissolvens comb. nov. and Enterobacter nimipressuralis comb. nov. J Clin Microbiol 23: 1114-1120.
4. Hoffmann H & Roggenkamp A (2003) Population genetics of the nomenspecies Enterobacter cloacae. Appl Environ Microbiol 69: 5306-5318.
5. Hoffmann H, Stindl S, Ludwig W et al. (2005a) Enterobacter hormaechei subsp. oharae subsp. nov., E. hormaechei subsp. hormaechei comb. nov., and E. hormaechei subsp. steigerwaltii subsp. nov., three new subspecies of clinical importance. J Clin Microbiol 43: 3297-3303.
6. Hoffmann H, Stindl S, Stumpf A, Mehlen A, Monget D, Heesemann J, Schleifer KH & Roggenkamp A (2005b) Description of Enterobacter ludwigii sp. nov., a novel Enterobacter species of clinical relevance. Syst Appl Microbiol 28: 206-212.
7. Hoffmann H, Stindl S, Ludwig W, Stumpf A, Mehlen A, Heesemann J, Monget D, Schleifer KH & Roggenkamp A (2005c) Reassignment of Enterobacter dissolvens to Enterobacter cloacae as E. cloacae subspecies dissolvens comb. nov. and emended description of Enterobacter asburiae and Enterobacter kobei. Syst Appl Microbiol 28: 196-205.
8. Kosako Y, Tamura K, Sakazaki R & Miki K (1996) Enterobacter kobei sp. nov., a new species of the family Enterobacteriaceae resembling Enterobacter cloacae. Curr Microbiol 33: 261-265.
9. Mellmann A, Bimet F, Bizet C et al. (2009) High interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry-based species identification of non-fermenting bacteria. J Clin Microbiol 47: 3732-3734.
10. Morand PC, Billoet A, Rottman M et al. (2009) Specific distribution among the Enterobacter cloacae complex of strains isolated from infected orthopedic implants. J Clin Microbiol 47: 2489-2495.
11. Neto JR, Yano T, Beriam LOS, Destéfano SAL, Oliveira VM & Rosato YB (2003) Comparative RFLP-ITS analysis between Enterobacter cloacae strains isolated from plants and clinical origin. Arq Inst Biol 70: 367-372.
12. O'Hara CM, Steigerwalt AG, Hill BC, Farmer JJ III, Fanning GR & Brenner DJ (1989) Enterobacter hormaechei, a new species of the family Enterobacteriaceae formerly known as enteric group 75. J Clin Microbiol 27: 2046-2049.
13. Paauw A, Caspers MP, Schuren FH, Leverstein-van Hall MA, Delétoile A, Montijn RC, Verhoef J & Fluit AC (2008) Genomic diversity within the Enterobacter cloacae complex. PLoS ONE 21: e3018.
14. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29: 2002-2007.
15. Pham HN, Ohkusu K, Mishima N, Noda M, Monir Shah M, Sun X, Hayashi M & Ezaki T (2007) Phylogeny and species identification of the family Enterobacteriaceae based on dnaJ sequences. Diagn Microbiol Infect Dis 58: 153-161.
16. Sanders WE Jr & Sanders CC (1997) Enterobacter spp.: pathogens poised to flourish at the turn of the century. Clin Microbiol Rev 10: 220-241.
17. Seeberg AH, Tolxdorff-Neutzling RM & Wiedemann B (1983) Chromosomal beta-lactamases of Enterobacter cloacae are responsible for resistance to third-generation cephalosporins. Antimicrob Agents Chemother 23: 918-925.
18. Tzelepi E, Giakkoupi P, Sofianou D, Loukova V, Kemeroglou A & Tsakris A (2000) Detection of extended-spectrum β-lactamases in clinical isolates of Enterobacter cloacae and Enterobacter aerogenes. J Clin Microbiol 38: 542-546.