Coexpression of chaperonin GroEL/GroES markedly enhanced soluble and functional expression of recombinant human interferon-gamma in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 93, Issue 3, 2012, Pages 1065-1074

  Yan, Xiao ^a   Hu, Sheng ^a   Guan, Yi Xin ^a   Yao, Shan Jing ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

GroEL GroES coexpression system; Protein folding; Protein protein interactions; Recombinant human interferon gamma; Soluble expression

Indexed keywords

BIOLOGICAL ACTIVITIES; CHAPERONIN; CHEMICAL CROSS-LINKING; CIRCULAR DICHROISM SPECTRA; CLINICAL THERAPY; CO-EXPRESSION; CO-EXPRESSION SYSTEMS; CULTURE TEMPERATURE; CYTOPLASMIC INCLUSION; E. COLI; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPRESSION LEVELS; EXPRESSION VECTORS; FERMENTATION BROTHS; FLUORESCENCE SPECTRA; FUNCTIONAL EXPRESSION; GROEL/GROES; INCUBATION TIME; INTERFERON-GAMMA; L-ARABINOSE; OPTIMIZED CONDITIONS; POSITIVE CORRELATIONS; PROTEIN-PROTEIN INTERACTIONS; RECOMBINANT E. COLI; RECOMBINANT PROTEIN; SOLUBLE EXPRESSION; THREE-DIMENSIONAL STRUCTURE; YEAST TWO-HYBRID SYSTEM;

ANTIBIOTICS; CROSSLINKING; DICHROISM; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; GLYCOPROTEINS; HYBRID SYSTEMS; PROTEIN FOLDING; SIZE EXCLUSION CHROMATOGRAPHY;

INTERFERONS;

ARABINOSE; CHAPERONIN; HUMAN INTERFERON; ISOPROPYL THIOGALACTOSIDE; RECOMBINANT GAMMA INTERFERON;

BIOACTIVITY; BIOASSAY; CHROMATOGRAPHY; COLIFORM BACTERIUM; FERMENTATION; FLUORESCENCE; GAMMA RAY RADIATION; GENE EXPRESSION; HYBRID; PROTEIN; RECOMBINATION; SOLUBILIZATION; THREE-DIMENSIONAL MODELING;

ARTICLE; CIRCULAR DICHROISM; CONCENTRATION (PARAMETERS); CONFORMATIONAL TRANSITION; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FERMENTATION; FLUORESCENCE SPECTROSCOPY; GEL PERMEATION CHROMATOGRAPHY; INCUBATION TIME; NONHUMAN; PLASMID; PROTEIN CROSS LINKING; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; TEMPERATURE; TWO HYBRID SYSTEM; YEAST;

BIOTECHNOLOGY; CHAPERONIN 10; CHAPERONIN 60; CULTURE MEDIA; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FERMENTATION; GENE EXPRESSION REGULATION, BACTERIAL; HUMANS; INTERFERON-GAMMA; PLASMIDS; PROTEIN FOLDING; RECOMBINANT PROTEINS; SOLUBILITY; TWO-HYBRID SYSTEM TECHNIQUES;

ESCHERICHIA COLI;

EID: 84856404197     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3599-2     Document Type: Article

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