Selective expression of the soluble product fraction in Escherichia coli cultures employed in recombinant protein production processes

Applied Microbiology and Biotechnology

Volumn 87, Issue 6, 2010, Pages 2047-2058

  Gnoth, Stefan ^a   Simutis, Rimvydas ^b   Lübbert, Andreas ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^b KAUNAS UNIVERSITY OF TECHNOLOGY   (Lithuania)

Author keywords

Feedback control; Hybrid artificial neural network; Optimization; PAT; Process model; Specific biomass growth rate; Temperature

Indexed keywords

BIOMASS GROWTH RATE; CLOSED-LOOP CONTROL; CONTROL MEASURES; CULTIVATION PROCEDURE; CULTURE TEMPERATURE; DYNAMIC PROCESS; FED-BATCH FERMENTATION; FUNCTIONAL PROTEINS; HYBRID ARTIFICIAL NEURAL NETWORK; INCLUSION BODIES; PAT; PROCESS MODEL; PROCESS VALIDATION; PRODUCT FORMATION; PRODUCT FRACTION; REAL PROCESS; RECOMBINANT PROTEIN; RECOMBINANT PROTEIN PRODUCTIONS; REFOLDING PROCEDURES; REPRODUCIBILITIES; SOLUBLE PROTEINS; SPECIFIC GROWTH RATE; TARGET PROTEINS; TEMPERATURE PROFILES;

CELL CULTURE; DISSOLUTION; ESCHERICHIA COLI; FEEDBACK CONTROL; FERMENTATION; GROWTH RATE; NEURAL NETWORKS; OPTIMIZATION; PROTEINS; ROBUST CONTROL;

PROCESS CONTROL;

PROTEIN; RECOMBINANT PROTEIN;

ARTIFICIAL NEURAL NETWORK; BIOMASS; COLIFORM BACTERIUM; CYTOPLASM; EXPERIMENTAL STUDY; FEEDBACK MECHANISM; GENE EXPRESSION; GROWTH RATE; OPTIMIZATION; PROTEIN; REACTION KINETICS; RECOMBINATION; TEMPERATURE PROFILE;

ARTICLE; ARTIFICIAL NEURAL NETWORK; BACTERIUM CULTURE; CONTROLLED STUDY; ESCHERICHIA COLI; FED BATCH FERMENTATION; GROWTH RATE; NONHUMAN; PROCESS MODEL; TEMPERATURE;

ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; INCLUSION BODIES; KINETICS; RECOMBINANT PROTEINS; SOLUBILITY; TEMPERATURE;

ESCHERICHIA COLI;

EID: 77955552228     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2608-1     Document Type: Article

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