Identification of Immune Cells by Flow Cytometry in Vaginal Lavages from Women with Vulvovaginitis and Normal Microflora

American Journal of Reproductive Immunology

Volumn 67, Issue 3, 2012, Pages 198-205

  Giraldo, Paulo César ^a   de Carvalho, Joziani Beghini Junqueira ^a   do Amaral, Rose Luce Gomes ^a   da Silveira Gonçalves, Ana Katherine ^b   Eleutério, José ^c   Guimarães, Fernando ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

^b FEDERAL UNIVERSITY OF RIO GRANDE DO NORTE   (Brazil)

^c FEDERAL UNIVERSITY OF CEARÁ   (Brazil)

Author keywords

Bacterial vaginosis; Flow cytometry; Immunity; Leukocytes; Vagina; Vulvovaginal candidiasis

Indexed keywords

FLUOROCHROME; MONOCLONAL ANTIBODY;

ADULT; ARTICLE; B LYMPHOCYTE; CD4+ T LYMPHOCYTE; CD8+ T LYMPHOCYTE; CONTROLLED STUDY; FEMALE; FLOW CYTOMETRY; HUMAN; IMMUNOCOMPETENT CELL; LEUKOCYTE; MACROPHAGE; MAJOR CLINICAL STUDY; MICROFLORA; NATURAL KILLER T CELL; NEUTROPHIL; OUTCOME ASSESSMENT; PRIORITY JOURNAL; VAGINA CANDIDIASIS; VAGINAL LAVAGE; VULVOVAGINITIS;

ADULT; CANDIDIASIS, VULVOVAGINAL; FEMALE; FLOW CYTOMETRY; HUMANS; VAGINA; VAGINAL DOUCHING; VAGINOSIS, BACTERIAL;

EID: 84856370187     PISSN: 10467408     EISSN: 16000897     Source Type: Journal    
DOI: 10.1111/j.1600-0897.2011.01093.x     Document Type: Article

References (41)

1. Duarte G, Landers DV: Vulvovaginites: aspectos epidemiológicos. J Bras Doenças Sex Transm 1998; 9:4-14.
2. ACOG technical bulletin: Vaginitis. Number 226 - July 1996 (replaces No. 221, March 1996). committee on technical bulletins of the American college of obstetricians and gynecologists. Int J Gynaecol Obstet 1996; 54:293-302.
3. Nyirjesy P: Vulvovaginal candidiasis and bacterial vaginosis. Infect Dis Clin N Am 2008; 22:637-652.
4. Sobel JD: Bacterial vaginosis. Ann Rev Med 2000; 51:349-356.
5. Verstraelen H, Verhelst R, Claeys G, Temmerman M, Vaneechoutte M: Culture-independent analysis of vaginal microflora: the unrecognized association of Atopobium vaginae with bacterial vaginosis. Am J Obstet Gynecol 2004; 191:1130-1132.
6. Centers for Disease Control and Prevention: Sexually Transmitted Diseases Treatment Guidelines 2010. Diseases Characterized by Vaginal Discharge. Bacterial Vaginosis.
7. St John E, Mares D, Spear GT: Bacterial vaginosis and host immunity. Curr HIV/AIDS Rep 2007; 4:22-28.
8. Sweet RL: Gynecologic conditions and bacterial vaginosis: implications for the non-pregnant patient. Infect Dis Obstet Gynecol 2000; 8:184-190.
9. Guaschino S, De Santo D, De Seta F: New perspectives in antibiotic prophylaxis for obstetric and gynecologic surgery. J Hosp Infect 2002; 50(Suppl A):S13-S16.
10. Sewankambo N, Gray RH, Wawer MJ, Paxton L, McNaim D, Wabwire-Mangen F, Serwadda D, Li C, Kiwanuka N, Hillier SL, Rabe L, Gaydos CA, Quinn TC, Konde-Lule J: HIV-1 infection associated with abnormal vaginal flora morphology and bacterial vaginosis. Lancet 1997; 350:546-550.
11. McGregor JA, French JI: Bacterial vaginosis in pregnancy. Obstet Gynecol Surv 2000; 55:S1-S19.
12. Donders G: Diagnosis and management of bacterial vaginosis and other types of abnormal vaginal bacterial flora: a review. Obstet Gynecol Surv 2010; 65:462-473.
13. Cauci S, Guachino S, De Aloysio D, Driussi S, De Santo D, Penacchioni P, Quadrifoglio F: Interrelationships of interleukin-8 with interleukin-1beta and neutrophils in vaginal fluid of healthy and bacterial vaginosis positive women. Mol Hum Reprod 2003; 9:53-58.
14. Sobel JD: Vulvovaginal candidosis. Lancet 2007; 369:1961-1971.
15. Giraldo PC, Gonçalves AKS, Eleutério Jr J: Mecanismos de defesa da mucosa genital feminina. In Infecção genital na mulher, S Peixoto (ed.). São Paulo, Editora Roca Ltda, 2005, pp 37-41.
16. Fahey JV, Sentman CL, Pioli PA, Shen L: Innate and adaptive immunity in female genital tract: cellular responses and interactions. Immunol Rev 2005; 206:306-335.
17. Ledger WJ, Witkin SS: Vaginal immunity. In Vulvovaginal infections, WJ Ledger, SS Witkin (eds). Washington, ASM Press, 2007, pp 12-15.
18. Soehnlein O, Lindbom L: Phagocyte partnership during the onset and resolution of inflammation. Nature Rev Immunol 2010; 10:427-439.
19. Giraldo PC, Feitoza SBN, Gonçalves AKS, Cornetta MCM, EleutérioJr J, Tristão AR: A resposta imune celular da mucosa vaginal às vulvovaginites. J bras Doenças Sex Transm 2006; 18:263-265.
20. Giraldo PC, Gonçalves AKS, Linhares IM, Cornetta MCM, Giraldo HPD: Corrimento genital. Diagnósticos clínicos e laboratoriais. In Patologia do trato genital inferior, NV Martins, JCL Ribalta (eds). São Paulo, Editora Roca Ltda, 2005, pp 124-129.
21. Balcells Gorina A: Exames de sangue. Hematologia clínica. In A clínica e o laboratório, A Balcells Gorina (ed.). Rio de Janeiro, Medsi, 1996, pp 121-158.
22. Balcells Gorina A: Exame do líquido cefalorraquidiano. In A clínica e o laboratório, A Balcells Gorina (ed.). Rio de Janeiro, Medsi, 1996, pp 213-219.
23. Givan AL, White HD, Stern JE, Colby E, Gosselin EJ, Guyre PM, Wira CR: Flow cytometric analysis of leukocytes in the human female reproductive tract: comparison of fallopian tube, uterus, cervix, and vagina. Am J Reprod Immunol 1997; 38:350-359.
24. Patton DL, Thwin SS, Meir A, Hooton TM, Stapleton AE, Eschenbach DA: Epithelial cell layer thickness and immune cell populations in the normal human vagina at different stages of the menstrual cycle. Am J Obstet Gynecol 2000; 183:967-973.
25. White HD, Prabhala RH, Humphrey SL, Crassi KM, Richardson JM, Wira CR: A method for the dispersal and characterization of leukocytes from the human female reproductive tract. Am J Reprod Immunol 2000; 44:96-103.
26. Ma Z, Lü FX, Torten M, Miller CJ: The number and distribution of immune cells in the cervicovaginal mucosa remain constant throughout the menstrual cycle of rhesus macaques. Clin Immunol 2001; 100:240-249.
27. Ildgruben AK, Sjöberg IM, Hammarström ML: Influence of hormonal contraceptives on the immune cells and thickness of human vaginal epithelium. Obstet Gynecol 2003; 102:571-582.
28. Feitoza SBN, Giraldo PC, Gonçalves AKS, Amaral RLG, Eleutério Jr J, Tristão A, Cornetta MCM: Identificação e quantificação de células de defesa na mucosa vaginal de mulheres com vaginose bacteriana, candidíase vaginal e sem vulvovaginites. Rev Bras Genitoscopia 2009; 3:76-79.
29. Di Fabio S, Corrias F, Monardo F, Titti F: Flow citometry analysis of immune cell populations isolated from cervicovaginal secretions of cynomolgus monkeys. J Immunol Methods 2004; 2:7-14.
30. Schultheiss T, Stolte-Leeb N, Sopper S, Stahl-Hennig C: Flow cytometric characterization of the lymphocyte composition in a variety of mucosal tissues in healthy rhesus macaques. J Med Primatol 2011; 40:41-51.
31. Amsel R, Totten PA, Spiegel CA, Chen KC, Eschenbach D, Holmes KK: Nonspecific vaginitis: diagnostic criteria and microbial and epidemiologic associations. Am J Med 1983; 74:14-22.
32. Nugent RP, Krohn MA, Hillier SL: Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation. J Clin Microbiol 1991; 29:297-301.
33. Spiegel CA, Amsel R, Holmes KK: Diagnosis of bacterial vaginosis by direct Gram staim of vaginal fluid. J Clin Microbiol 1983; 18:170-177.
34. Giraldo P, von Nowaskonski A, Gomes FA, Linhares I, Neves NA, Witkin SS: Vaginal colonization by Candida in asymptomatic women with and without a history of recurrent vulvovaginal candidiasis. Obstet Gynecol 2000; 95:413-416.
35. Fidel Jr PL: History and update on host defense against vaginal candidiasis. Am J Reprod Immunol 2007; 57:2-12.
36. Mirmonsef P, Gilbert D, Zariffard MR, Hamaker BR, Kaur A, Landay AL, Spear GT: The effects of commensal bacteria on innate immune responses in the female genital tract. Am J Reprod Immunol 2011; 65:190-195.
37. Wilson WH: International consensus recommendations on the flow cytometric immunophenotypic analysis of hematolymphoid neoplasia. Cytometry B Clin Cytom 2007; 72(Suppl 1):S2.
38. Davis BH, Holden JT, Bene MC, Borowitz MJ, Braylan RC, Cornfield D, Gorczyca W, Lee R, Maiese R, Orfao A, Wells D, Wood BL, Stetler-Stevenson M: 2006 Bethesda international consensus recommendations on the flow cytometric immunophenotypic analysis of hematolymphoid neoplasia: medical indications. Cytometry B Clin Cytom 2007; 72(Suppl 1):S5-S13.
39. Stetler-Stevenson M, Davis B, Wood B, Braylan R: 2006 Bethesda international consensus conference on flow cytometric immunophenotyping of hematolymphoid neoplasia. Cytometry B Clin Cytom 2007; 72(Suppl 1):S3.
40. Craig FE, Foon KA: Flow cytometric immunophenotyping for hematologic neoplasms. Blood 2008; 111:3941-3967.
41. Hengel RL, Nicholson JK: An update on the use of flow cytometry in HIV infection and AIDS. Clin Lab Med 2001; 21:841-856.