Development of a rotor-gene real-time PCR assay for the detection and quantification of Mycoplasma genitalium

Journal of Microbiological Methods

Volumn 88, Issue 2, 2012, Pages 311-315

  Müller, Etienne E ^a   Venter, Johanna M E ^a   Magooa, Mahlape P ^a   Morrison, Charles ^b   Lewis, David A ^a,c,d   Mavedzenge, Sue Napierala ^e,f  

^a NATIONAL INSTITUTE FOR COMMUNICABLE DISEASES   (South Africa)

^b FAMILY HEALTH INTERNATIONAL   (United States)

^c UNIVERSITY OF THE WITWATERSRAND   (South Africa)

^d UNIVERSITY OF CAPE TOWN   (South Africa)

^e RTI INTERNATIONAL   (United States)

^f LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

Author keywords

Bacterial load; Endocervical swabs; Mycoplasma genitalium; Real time quantitative PCR

Indexed keywords

BACTERIAL DNA; DIHYDROLIPOAMIDE DEHYDROGENASE;

ARTICLE; BACTERIAL COUNT; BACTERIAL LOAD; BACTERIUM DETECTION; CLINICAL RESEARCH; CONTROLLED STUDY; GENE; GENE AMPLIFICATION; LIMIT OF DETECTION; MYCOPLASMA GENITALIUM; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; ROTOR GENE; SENSITIVITY AND SPECIFICITY; UREAPLASMA; UTERINE CERVIX CYTOLOGY; ZIMBABWE;

BACTERIAL LOAD; CERVIX UTERI; DNA, BACTERIAL; FEMALE; HUMANS; MYCOPLASMA GENITALIUM; MYCOPLASMA INFECTIONS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; ZIMBABWE;

BACTERIA (MICROORGANISMS); MYCOPLASMA; MYCOPLASMA GENITALIUM; UREAPLASMA;

EID: 84856365783     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2011.12.017     Document Type: Article

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