Isolation of Mycoplasma genitalium from first-void urine specimens by coculture with vero cells

Journal of Clinical Microbiology

Volumn 45, Issue 3, 2007, Pages 847-850

  Hamasuna, Ryoichi ^a,b   Osada, Yukio ^b   Jensen, Jørgen Skov ^a  

^a STATENS SERUM INSTITUT   (Denmark)

^b UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMPHOTERICIN B; AMPICILLIN; ANTIBIOTIC AGENT; BACTERIAL DNA; PENICILLIN G; POLYMYXIN B;

ANIMAL CELL; ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATION; CHLAMYDIA TRACHOMATIS; CLINICAL ARTICLE; GROWTH INHIBITION; HUMAN; MALE; MYCOPLASMA GENITALIUM; NEISSERIA GONORRHOEAE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEXUALLY TRANSMITTED DISEASE; UREAPLASMA UREALYTICUM; URETHRITIS; URINALYSIS; VERO CELL;

ANIMALS; ANTI-BACTERIAL AGENTS; BACTERIOLOGICAL TECHNIQUES; CERCOPITHECUS AETHIOPS; COCULTURE TECHNIQUES; HUMANS; JAPAN; MALE; MICROBIAL SENSITIVITY TESTS; MYCOPLASMA GENITALIUM; MYCOPLASMA INFECTIONS; URETHRITIS; URINE; VERO CELLS;

BACTERIA (MICROORGANISMS); CHLAMYDIA TRACHOMATIS; MYCOPLASMA GENITALIUM; NEISSERIA GONORRHOEAE; UREAPLASMA PARVUM; UREAPLASMA UREALYTICUM;

EID: 33947200740     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.02056-06     Document Type: Article

References (14)

1. Baseman, J. B., M. Cagle, J. E. Korte, C. Herrera, W. G. Rasmussen, J. G. Baseman, R. Shain, and J. M. Piper. 2004. Diagnostic assessment of Mycoplasma genitalium in culture-positive women. J. Clin. Microbiol. 42:203-211.
2. Deguchi, T., T. Yoshida, S. Yokoi, M. Ito, M. Tamaki, H. Ishiko, and S.-I. Maeda. 2002. Longitudinal quantitative detection by real-time PCR of Mycoplasma genitalium in first-pass urine of men with recurrent nongonococcal urethritis. J. Clin. Microbiol. 40:3854-3856.
3. Freundt, E. A. 1983. Culture media for classic mycoplasmas. Methods Mycoplasmol. 1:127-135.
4. Hamasuna, R., Y. Osada, and J. S. Jensen. 2005. Antibiotic susceptibility testing of Mycoplasma genitalium by TaqMan 5′ nuclease real-time PCR. Antimicrob. Agents Chemother. 49:4993-4998.
5. Hjorth, S. V., E. Björnelius, P. Lidbrink, L. Falk, B. Dohn, L. Berthelsen, L. Ma, D. H. Martin, and J. S. Jensen. 2006. Sequence-based typing of Mycoplasma genitalium reveals sexual transmission. J. Clin. Microbiol. 44:2078-2083.
6. Jensen, J. S., E. Björnelius, B. Dohn, and P. Lidbrink. 2004. Comparison of first void urine and urogenital swab specimens for detection of Mycoplasma genitalium and Chlamydia trachomatis by polymerase chain reaction in patients attending a sexually transmitted disease clinic. Sex. Transm. Dis. 31: 499-507.
7. Jensen, J. S., E. Björnelius, B. Dohn, and P. Lidbrink. 2004. Use of TaqMan 5′ nuclease real-time PCR for quantitative detection of Mycoplasma genitalium DNA in males with and without urethritis who were attendees at a sexually transmitted disease clinic. J. Clin. Microbiol. 42:683-692.
8. Jensen, J. S., H. T. Hansen, and K. Lind. 1996. Isolation of Mycoplasma genitalium strains from the male urethra. J. Clin. Microbiol. 34:286-291.
9. Jurstrand, M., J. S. Jensen, H. Fredlund, L. Falk, and P. Molling. 2005. Detection of Mycoplasma genitalium in urogenital specimens by real-time PCR and by conventional PCR assay. J. Med. Microbiol. 54:23-29.
10. Luo, D., W. Xu, G. Liang, S. Wang, Z. Wang, Z. Bi, and W. Zhu. 1999. Isolation and identification of Mycoplasma genitalium from high risk populations of sexually transmitted diseases in China. Chin. Med. J. 112:489-492.
11. Taylor-Robinson, D., J. G. Tully, P. M. Furr, R. M. Cole, D. L. Rose, and N. F. Hanna. 1981. Urogenital mycoplasma infection of man: a review with observations on a recently discovered mycoplasma. Isr. J. Med. Sci. 17:524-530.
12. Totten, P. A., M. A. Schwartz, K. E. Sjostrom, G. E. Kenny, H. H. Handsfield, J. B. Weiss, and W. L. Whittington. 2001. Association of Mycoplasma genitalium with nongonococcal urethritis in heterosexual men. J. Infect. Dis. 183:269-276.
13. Tully, J. G., D. L. Rose, J. B. Baseman, S. F. Dallo, A. L. Lazzell, and C. P. Davis. 1995. Mycoplasma pneumoniae and Mycoplasma genitalium mixture in synovial fluid isolate. J. Clin. Microbiol. 33:1851-1855.
14. Tully, J. G., D. Taylor-Robinson, R. M. Cole, and D. L. Rose. 1981. A newly discovered mycoplasma in the human urogenital tract. Lancet i:1288-1291.