Factor VIII light chain contains a binding site for factor X that contributes to the catalytic efficiency of factor xase

Biochemistry

Volumn 51, Issue 3, 2012, Pages 820-828

  Takeyama, Masahiro ^a   Wakabayashi, Hironao ^a   Fay, Philip J ^a  

^a UNIVERSITY OF ROCHESTER SCHOOL OF MEDICINE AND DENTISTRY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATED PROTEIN C; APPARENT K; BINDING ASSAYS; CATALYTIC EFFICIENCIES; CHEMICAL CROSS-LINKING; CLEAVAGE RATES; COFACTORS; COOH-TERMINAL; ELISA-BASED ASSAYS; FACTOR VIII; FACTOR X; FACTOR XASE; HIGH AFFINITY; LIGHT CHAIN; SYNTHETIC PEPTIDE; TRIPLE MUTANTS; WESTERN BLOTTING;

CATALYSIS; CROSSLINKING; SURFACE PLASMON RESONANCE;

BINDING SITES;

ACTIVATED PROTEIN C; ARGININE; BLOOD CLOTTING FACTOR 10; BLOOD CLOTTING FACTOR 8; BLOOD CLOTTING FACTOR 8A; RECOMBINANT BLOOD CLOTTING FACTOR 8;

ARTICLE; BINDING AFFINITY; BINDING ASSAY; BINDING SITE; CATALYSIS; CHEMICAL MUTAGENESIS; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; LIGHT CHAIN; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN CROSS LINKING; SOLID PHASE SYNTHESIS; SURFACE PLASMON RESONANCE; WESTERN BLOTTING;

CATALYTIC DOMAIN; CYSTEINE ENDOPEPTIDASES; FACTOR VIII; FACTOR X; HUMANS; MUTAGENESIS; NEOPLASM PROTEINS; PROTEIN BINDING; PROTEIN SUBUNITS; RECOMBINANT PROTEINS;

EID: 84856287741     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi201731p     Document Type: Article

References (31)

1. Mann, K. G., Nesheim, M. E., Church, W. R., Haley, P., and Krishnaswamy, S. (1990) Surface-dependent reactions of the vitamin K-dependent enzyme complexes Blood 76, 1-16 (Pubitemid 20220784)
2. Miyahara, N., Shoda, J., Ishige, K., Kawamoto, T., Ueda, T., Taki, R., Ohkohchi, N., Hyodo, I., Thomas, M. B., Krishnamurthy, S., Carraway, K. L., and Irimura, T. (2008) MUC4 interacts with ErbB2 in human gallbladder carcinoma: potential pathobiological implications Eur. J. Cancer 44, 1048-1056
3. Vehar, G. A., Keyt, B., Eaton, D., Rodriguez, H., OBrien, D. P., Rotblat, F., Oppermann, H., Keck, R., Wood, W. I., and Harkins, R. N. 1984, Structure of human factor VIII Nature 312, 337-342 (Pubitemid 15209310)
4. Andersson, L. O., Forsman, N., Huang, K., Larsen, K., Lundin, A., Pavlu, B., Sandberg, H., Sewerin, K., and Smart, J. (1986) Isolation and characterization of human factor VIII: molecular forms in commercial factor VIII concentrate, cryoprecipitate, and plasma Proc. Natl. Acad. Sci. U. S. A. 83, 2979-2983 (Pubitemid 16041785)
5. Fay, P. J., Anderson, M. T., Chavin, S. I., and Marder, V. J. (1986) The size of human factor VIII heterodimers and the effects produced by thrombin Biochim. Biophys. Acta 871, 268-278 (Pubitemid 16056673)
6. Eaton, D., Rodriguez, H., and Vehar, G. A. (1986) Proteolytic processing of human factor VIII. Correlation of specific cleavages by thrombin, factor Xa, and activated protein C with activation and inactivation of factor VIII coagulant activity Biochemistry 25, 505-512 (Pubitemid 16053817)
7. Fay, P. J., Mastri, M., Koszelak, M. E., and Wakabayashi, H. (2001) Cleavage of factor VIII heavy chain is required for the functional interaction of a2 subunit with factor IXA J. Biol. Chem. 276, 12434-12439
8. Regan, L. M. and Fay, P. J. (1995) Cleavage of factor VIII light chain is required for maximal generation of factor VIIIa activity J. Biol. Chem. 270, 8546-8552
9. Donath, M. S., Lenting, P. J., van Mourik, J. A., and Mertens, K. (1995) The role of cleavage of the light chain at positions Arg1689 or Arg1721 in subunit interaction and activation of human blood coagulation factor VIII J. Biol. Chem. 270, 3648-3655
10. Nogami, K., Wakabayashi, H., and Fay, P. J. (2003) Mechanisms of factor Xa-catalyzed cleavage of the factor VIIIa A1 subunit resulting in cofactor inactivation J. Biol. Chem. 278, 16502-16509 (Pubitemid 36799510)
11. Koszelak Rosenblum, M. E., Schmidt, K., Freas, J., Mastri, M., and Fay, P. J. (2002) Cofactor activities of factor VIIIa and A2 subunit following cleavage of A1 subunit at Arg336 J. Biol. Chem. 277, 11664-11669 (Pubitemid 34952717)
12. Nogami, K., Wakabayashi, H., Schmidt, K., and Fay, P. J. (2003) Altered interactions between the A1 and A2 subunits of factor VIIIa following cleavage of A1 subunit by factor Xa J. Biol. Chem. 278, 1634-1641 (Pubitemid 36801394)
13. Lapan, K. A. and Fay, P. J. (1997) Localization of a factor X interactive site in the A1 subunit of factor VIIIa J. Biol. Chem. 272, 2082-2088 (Pubitemid 27058483)
14. Lamphear, B. J. and Fay, P. J. (1992) Proteolytic interactions of factor IXa with human factor VIII and factor VIIIa Blood 80, 3120-3126 (Pubitemid 23004516)
15. Walker, F. J. and Fay, P. J. (1992) Regulation of blood coagulation by the protein C system FASEB J. 6, 2561-2567
16. Eaton, D. L., Wood, W. I., Eaton, D., Hass, P. E., Hollingshead, P., Wion, K., Mather, J., Lawn, R. M., Vehar, G. A., and Gorman, C. (1986) Construction and characterization of an active factor VIII variant lacking the central one-third of the molecule Biochemistry 25, 8343-8347 (Pubitemid 17003930)
17. Fay, P. J., Smudzin, T. M., and Walker, F. J. (1991) Activated protein C-catalyzed inactivation of human factor VIII and factor VIIIa. Identification of cleavage sites and correlation of proteolysis with cofactor activity J. Biol. Chem. 266, 20139-20145 (Pubitemid 21908437)
18. Krishnaswamy, S., Williams, E. B., and Mann, K. G. (1986) The binding of activated protein C to factors V and Va J. Biol. Chem. 261, 9684-9693 (Pubitemid 17214094)
19. Fay, P. J. and Walker, F. J. (1989) Inactivation of human factor VIII by activated protein C: evidence that the factor VIII light chain contains the activated protein C binding site Biochim. Biophys. Acta 994, 142-148 (Pubitemid 19030817)
20. Walker, F. J., Scandella, D., and Fay, P. J. (1990) Identification of the binding site for activated protein C on the light chain of factors V and VIII J. Biol. Chem. 265, 1484-1489
21. Nogami, K., Lapan, K. A., Zhou, Q., Wakabayashi, H., and Fay, P. J. (2004) Identification of a factor Xa-interactive site within residues 337-372 of the factor VIII heavy chain J. Biol. Chem. 279, 15763-15771 (Pubitemid 38509261)
22. Wakabayashi, H., Koszelak, M. E., Mastri, M., and Fay, P. J. (2001) Metal ion-independent association of factor VIII subunits and the roles of calcium and copper ions for cofactor activity and inter-subunit affinity Biochemistry 40, 10293-10300 (Pubitemid 32800136)
23. Ansong, C. and Fay, P. J. (2005) Factor VIII A3 domain residues 1954-1961 represent an A1 domain-interactive site Biochemistry 44, 8850-8857 (Pubitemid 40840450)
24. Nogami, K., Zhou, Q., Myles, T., Leung, L. L., Wakabayashi, H., and Fay, P. J. (2005) Exosite-interactive regions in the A1 and A2 domains of factor VIII facilitate thrombin-catalyzed cleavage of heavy chain J. Biol. Chem. 280, 18476-18487 (Pubitemid 41389097)
25. Lollar, P., Fay, P. J., and Fass, D. N. (1993) Factor VIII and factor VIIIa Methods Enzymol. 222, 128-143 (Pubitemid 23303231)
26. OBrien, L. M., Mastri, M., and Fay, P. J. (2000) Regulation of factor VIIIa by human activated protein C and protein S: inactivation of cofactor in the intrinsic factor Xase Blood 95, 1714-1720 (Pubitemid 30120834)
27. Carraway, K. L. and Koshland, D. E. (1972) Carbodiimide modification of proteins Methods Enzymol. 25, 616-623
28. Ngo, J. C., Huang, M., Roth, D. A., Furie, B. C., and Furie, B. (2008) Crystal structure of human factor VIII: implications for the formation of the factor IXa-factor VIIIa complex Structure 16, 597-606 (Pubitemid 351458707)
29. Shen, B. W., Spiegel, P. C., Chang, C. H., Huh, J. W., Lee, J. S., Kim, J., Kim, Y. H., and Stoddard, B. L. (2008) The tertiary structure and domain organization of coagulation factor VIII Blood 111, 1240-1247 (Pubitemid 351213406)
30. Lenting, P. J., van de Loo, J. W., Donath, M. J., van Mourik, J. A., and Mertens, K. (1996) The sequence Glu1811-Lys1818 of human blood coagulation factor VIII comprises a binding site for activated factor IX J. Biol. Chem. 271, 1935-1940
31. Wakabayashi, H. and Fay, P. J. (2008) Identification of residues contributing to A2 domain-dependent structural stability in factor VIII and factor VIIIa J. Biol. Chem. 283, 11645-11651