In vitro stoichiometry of complexes between the soluble RANK ligand and the monoclonal antibody denosumab

Biochemistry

Volumn 51, Issue 3, 2012, Pages 795-806

  Arthur, Kelly K ^a   Gabrielson, John P ^a   Hawkins, Nessa ^a,b   Anafi, Dan ^a,c   Wypych, Jette ^a   Nagi, Athena ^a   Sullivan, John K ^a   Bondarenko, Pavel V ^a  

^a AMGEN INC   (United States)

^b Neurozon LLC   (United States)

^c Oncobiologics   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTICAL ULTRACENTRIFUGATION; ANTIBODY MOLECULES; GAS-PHASE ANALYSIS; HIGH AFFINITY; HIGH CONCENTRATION; IN-VITRO; INTERMEDIATE SPECIE; OSTEOPROTEGERIN; RANK LIGANDS; SEDIMENTATION VELOCITIES; SEPARATION TECHNIQUES; STABLE COMPLEXES; STATIC LIGHT SCATTERING; THERAPEUTIC ANTIBODIES; TIME OF FLIGHT MASS SPECTROMETRY;

CENTRIFUGATION; CHEMICAL DETECTION; ELECTROSPRAY IONIZATION; GAS CHROMATOGRAPHY; IONIZATION OF GASES; LIGANDS; MASS SPECTROMETRY; MOLECULES; MONOCLONAL ANTIBODIES; OLIGOMERS; SIZE EXCLUSION CHROMATOGRAPHY; STOICHIOMETRY;

SIZE SEPARATION;

DENOSUMAB; OSTEOCLAST DIFFERENTIATION FACTOR; OSTEOPROTEGERIN; RECEPTOR ACTIVATOR OF NUCLEAR FACTOR KAPPA B;

ANTIGEN ANTIBODY COMPLEX; ANTIGEN BINDING; AQUEOUS SOLUTION; ARTICLE; BINDING AFFINITY; ELECTROSPRAY MASS SPECTROMETRY; GEL PERMEATION CHROMATOGRAPHY; IN VITRO STUDY; LIGHT SCATTERING; PRIORITY JOURNAL; PROTEIN ASSEMBLY; RECEPTOR BINDING; SEDIMENTATION; STOICHIOMETRY; TEMPERATURE; TIME OF FLIGHT MASS SPECTROMETRY; ULTRACENTRIFUGATION;

ANIMALS; ANTIBODIES, MONOCLONAL; BUFFERS; CHO CELLS; CRICETINAE; ESCHERICHIA COLI; GLYCOSYLATION; HUMANS; IMMUNOGLOBULIN G; PROTEIN INTERACTION MAPPING; PROTEIN STABILITY; RANK LIGAND; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

EID: 84856252552     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi2007806     Document Type: Article

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