A therapeutic antibody and its antigen form different complexes in serum than in phosphate-buffered saline: A study by analytical ultracentrifugation

Analytical Biochemistry

Volumn 388, Issue 2, 2009, Pages 279-287

  Demeule, Barthélemy ^a   Shire, Steven J ^a   Liu, Jun ^a  

^a GENENTECH INC   (United States)

Author keywords

Analytical ultracentrifugation; Biopharmaceutics; Characterization; Complex; Ex vivo; Fluorescence; IgE; Labeling; Omalizumab; Protein; rhuMabE25; Sedimentation velocity; Serum; Xolair

Indexed keywords

ASSOCIATION REACTIONS; CENTRIFUGATION; FLUORESCENCE; LABELING; MONOCLONAL ANTIBODIES; PROTEINS;

ANALYTICAL ULTRACENTRIFUGATION; BIOPHARMACEUTICS; COMPLEX; EX-VIVO; OMALIZUMAB; RHUMABE25; SEDIMENTATION VELOCITIES; SERUM; XOLAIR;

SIZE EXCLUSION CHROMATOGRAPHY;

ANTIBODY; ANTIGEN; FLUORESCENT DYE; IMMUNOGLOBULIN E ANTIBODY; MONOCLONAL ANTIBODY; OMALIZUMAB; PHOSPHATE BUFFERED SALINE;

ANIMAL CELL; AREA UNDER THE CURVE; ARTICLE; BLOOD ANALYSIS; BODY FLUID; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DRUG BINDING; DRUG POTENCY; FEMALE; FLUORESCENCE ANALYSIS; FLUORESCENCE DETECTION SYSTEM; GEL PERMEATION CHROMATOGRAPHY; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; SELF CONCEPT; SENSITIVITY AND SPECIFICITY; SERUM; ULTRACENTRIFUGATION;

ALEXA;

EID: 64549087965     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.03.012     Document Type: Article

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