Effects of resin ligand density on yield and impurity clearance in preparative cation exchange chromatography. I. Mechanistic evaluation

Journal of Chromatography A

Volumn 1225, Issue , 2012, Pages 62-69

  Fogle, Jace ^a   Mohan, Nina ^a   Cheung, Eric ^a   Persson, Josefine ^a  

^a GENENTECH INC   (United States)

Author keywords

Aggregates; Cation exchange chromatography; Charge variants; High molecular weight variants; Host cell protein; Ligand density; Monoclonal antibodies

Indexed keywords

CATION EXCHANGE CHROMATOGRAPHY; CHARGE VARIANTS; HIGH MOLECULAR WEIGHT; HOST CELL PROTEIN; LIGAND DENSITY;

AGGREGATES; CHROMATOGRAPHY; MOLECULAR WEIGHT; MONOCLONAL ANTIBODIES; POSITIVE IONS; PROTEINS; SURFACE PLASMON RESONANCE;

LIGANDS;

AGAROSE; CATION EXCHANGE RESIN; MONOCLONAL ANTIBODY;

ANIMAL CELL; ARTICLE; BINDING KINETICS; CLEARANCE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; DENSITY; ELUTION; GEL PERMEATION CHROMATOGRAPHY; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; LIGAND DENSITY; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PURIFICATION; SURFACE CHARGE;

ANIMALS; ANTIBODIES, MONOCLONAL; CATIONS; CHO CELLS; CHROMATOGRAPHY, ION EXCHANGE; CRICETINAE; CRICETULUS; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; ION EXCHANGE RESINS; LIGANDS; MOLECULAR WEIGHT; PROTEINS;

EID: 84856234304     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2011.12.048     Document Type: Article

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