A two-step fluorescence-activated cell sorting approach to isolate genetically modified mammalian cells with isopropyl-beta-D-thiogalactoside (IPTG)-inducible gene expression

Cytometry Part A

Volumn 81 A, Issue 2, 2012, Pages 101-104

  Mok, Myth T S ^a   Henderson, Beric R ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ISOPROPYL THIOGALACTOSIDE;

ANIMAL; ARTICLE; CELL SEPARATION; DRUG EFFECT; FLOW CYTOMETRY; GENE EXPRESSION; GENE VECTOR; GENETIC ENGINEERING; GENETIC TRANSFECTION; GENETICS; HUMAN; MAMMAL; METABOLISM; METHODOLOGY; MOUSE; TUMOR CELL LINE;

ANIMALS; CELL LINE, TUMOR; CELL SEPARATION; FLOW CYTOMETRY; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC VECTORS; HUMANS; ISOPROPYL THIOGALACTOSIDE; MAMMALS; MICE; TRANSFECTION;

EID: 84856197803     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22003     Document Type: Article

References (12)

1. MacLachlan TK, Somasundaram K, Sgagias M, Shifman Y, Muschel RJ, Cowan KH, El-Deiry WS.BRCA1 effects on the cell cycle and the DNA damage response are linked to altered gene expression.J Biol Chem 2000;275:2777-2785.
2. Fabbro M, Schuechner S, Au WW, Henderson BR.BARD1 regulates BRCA1 apoptotic function by a mechanism involving nuclear retention.Exp Cell Res 2004;298:661-673.
3. Lee JY, Lee YS, Kim JM, Kim KL, Lee JS, Jang HS, Shin IS, Suh W, Jeon ES, Byun J, et al.A novel chimeric promoter that is highly responsive to hypoxia and metals.Gene Ther 2006;13:857-868.
4. Jaalouk DE, Eliopoulos N, Couture C, Mader S, Galipeau J.Glucocorticoid-inducible retrovector for regulated transgene expression in genetically engineered bone marrow stromal cells.Hum Gene Ther 2000;11:1837-849.
5. Mok MT, Henderson BR.A comparison of BRCA1 nuclear localization with 14 DNA damage response proteins and domains: Identification of specific differences between BRCA1 and 53BP1 at DNA damage-induced foci.Cell Signal 2010;22:47-56.
6. Mathupala S, Sloan AA.An agarose-based cloning-ring anchoring method for isolation of viable cell clones.Biotechniques 2009;46:305-307.
7. Domann R, Martinez J.Alternative to cloning cylinders for isolation of adherent cell clones.Biotechniques 1995;18:594-595.
8. McFarland DC.Preparation of pure cell cultures by cloning.Methods Cell Sci 2000;22:63-66.
9. Luo X, Celler JW, Berndt A.LacSwitch inducible mammalian expression system in mouse Swiss 3T3 fibroblasts.Mol Cell Biochem 1999;200:127-132.
10. Fu Y, Ishii KK, Munakata Y, Saitoh T, Kaku M, Sasaki T.Regulation of tumor necrosis factor alpha promoter by human parvovirus B19 NS1 through activation of AP-1 and AP-2.J Virol 2002;76:5395-5403.
11. McGowan EM, Clarke CL.Effect of overexpression of progesterone receptor A on endogenous progestin-sensitive endpoints in breast cancer cells.Mol Endocrinol 1999;13:1657-1671.
12. Martin BR, Giepmans BN, Adams SR, Tsien RY.Mammalian cell-based optimization of the biarsenical-binding tetracysteine motif for improved fluorescence and affinity.Nat Biotechnol 2005;23:1308-1314.