An agarose-based cloning-ring anchoring method for isolation of viable cell clones

BioTechniques

Volumn 46, Issue 4, 2009, Pages 305-307

  Mathupala, Saroj P ^a   Sloan, Andrew E ^b  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY HOSPITALS OF CLEVELAND   (United States)

Author keywords

Cell cloning; Cell culture; Clonal isolation; Cloning rings; Mammalian cells; Tissue culture

Indexed keywords

AGAROSE; EDETIC ACID; TRYPSIN; SEPHAROSE;

ARTICLE; BIOENGINEERING; CELL CLONE; CELL CLONING; CELL COUNT; CELL CULTURE; CELL DAMAGE; CELL ISOLATION; CELL POPULATION; CELL STRAIN; CELL VIABILITY; CYTOLOGY; DESICCATION; EMBEDDING; GENOTYPE; MICROBIAL CONTAMINATION; MONOLAYER CULTURE; NONHUMAN; PHENOTYPIC VARIATION; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; CELL SEPARATION; CELL SURVIVAL; COMPARATIVE STUDY; CULTURE TECHNIQUE; HUMAN; METHODOLOGY; TEMPERATURE; TUMOR CELL LINE;

MAMMALIA;

CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; CELL SEPARATION; CELL SURVIVAL; CLONE CELLS; HUMANS; SEPHAROSE; TEMPERATURE;

EID: 67650509114     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113079     Document Type: Article

References (5)

1. Ian Freshney, R. 2000. Isolation of clones with cloning rings, Protocol 13.6, p. 204-206. In R. Ian Freshney (Ed.), Culture of Animal Cells: A Manual of Basic Technique, 4th ed., Wiley-Liss, Wilmington.
2. McFarland, D.C. 2000. Preparation of pure cell cultures by cloning. Methods Cell Sci. 22:63-66.
3. Martin, B.M. 1994. Cell Cloning, p. 143-151. In Tissue Culture Techniques: An Introduction. Birkhäuser, Basel.
4. Domann, R. and J. Martinez. 1995. Alternative to cloning cylinders for isolation of adherent cell clones. BioTechniques 18:592-594.
5. Ian Freshney, R. 2000. MTT-based cytotoxicity assay, Protocol 21.4, p. 336-338. In R. Ian Freshney (Ed.), Culture of Animal Cells: A Manual of Basic Technique, 4th ed. Wiley-Liss, Wilmington.