Cartilage-characteristic matrix reconstruction by sequential addition of soluble factors during expansion of human articular chondrocytes and their cultivation in collagen sponges

Tissue Engineering - Part C: Methods

Volumn 18, Issue 2, 2012, Pages 104-112

  Claus, Stéphanie ^a   Mayer, Nathalie ^a   Aubert Foucher, Elisabeth ^a   Chajra, Hanane ^b   Perrier Groult, Emeline ^a   Lafont, Jérôme ^a   Piperno, Muriel ^c   Damour, Odile ^a,d   Mallein Gerin, Frédéric ^a  

^a University Lyon i   (France)

^b Symatese Biomatériaux   (France)

^c CENTRE HOSPITALIER LYON SUD   (France)

^d EDOUARD HERRIOT HOSPITAL   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BODY FLUIDS; CARTILAGE; CELL CULTURE; CELLS; COLLAGEN; EXPANSION; GENE EXPRESSION; INSULIN; POLYMERASE CHAIN REACTION; TISSUE;

ARTICULAR CARTILAGES; BONE MORPHOGENETIC PROTEINS; CELL AMPLIFICATION; CELL EXPANSION; CHONDROCYTE REDIFFERENTIATION; CHONDROCYTES; COLLAGEN SCAFFOLDS; COLLAGEN SPONGE; FIBROBLAST GROWTH FACTOR; HUMAN ARTICULAR CHONDROCYTES; IMMUNOHISTOCHEMISTRY; IN-VITRO; MATRIX; ORGAN CULTURE; PROTEIN PRODUCTION; SEQUENTIAL ADDITION;

SCAFFOLDS (BIOLOGY);

MUS MUSCULUS;

BONE MORPHOGENETIC PROTEIN 2; COLLAGEN; FIBROBLAST GROWTH FACTOR 2; INSULIN; LIOTHYRONINE; SIGNAL PEPTIDE; TISSUE SCAFFOLD;

AGED; ANIMAL; ARTICLE; ARTICULAR CARTILAGE; CARTILAGE CELL; CATTLE; CELL CULTURE; CELL PROLIFERATION; CHEMISTRY; CHONDROGENESIS; CYTOLOGY; DRUG EFFECT; EXTRACELLULAR MATRIX; GENE EXPRESSION REGULATION; GENETICS; HUMAN; IMMUNOHISTOCHEMISTRY; METABOLISM; METHODOLOGY; MIDDLE AGED; MOUSE; PROTEIN SYNTHESIS; SOLUBILITY; TISSUE ENGINEERING;

AGED; ANIMALS; BONE MORPHOGENETIC PROTEIN 2; CARTILAGE, ARTICULAR; CATTLE; CELL PROLIFERATION; CELLS, CULTURED; CHONDROCYTES; CHONDROGENESIS; COLLAGEN; EXTRACELLULAR MATRIX; FIBROBLAST GROWTH FACTOR 2; GENE EXPRESSION REGULATION; HUMANS; IMMUNOHISTOCHEMISTRY; INSULIN; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; MICE; MIDDLE AGED; PROTEIN BIOSYNTHESIS; SOLUBILITY; TISSUE ENGINEERING; TISSUE SCAFFOLDS; TRIIODOTHYRONINE;

EID: 84856165883     PISSN: 19373384     EISSN: 19373392     Source Type: Journal    
DOI: 10.1089/ten.tec.2011.0259     Document Type: Article

References (27)

1. Brittberg, M., Lindahl, A., Nilsson, A., Ohlsson, C., Isaksson, O., and Peterson, L. Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med 331, 889, 1994.
2. Schnabel, M., Marlovits, S., Eckhoff, G., Fichtel, I., Gotzen, L., Vecsei, V., and Schlegel, J. Dedifferentiation-associated changes in morphology and gene expression in primary human articular chondrocytes in cell culture. Osteoarthritis Cartilage 10, 62, 2002.
3. Jakob, M., Demarteau, O., Schafer, D., Hintermann, B., Dick, W., Heberer, M., and Martin, I. Specific growth factors during the expansion and redifferentiation of adult human articular chondrocytes enhance chondrogenesis and cartilaginous tissue formation in vitro. J Cell Biochem 81, 368, 2001.
4. Martin, I., Suetterlin, R., Baschong, W., Heberer, M., Vunjak-Novakovic, G., and Freed, L.E. Enhanced cartilage tissue engineering by sequential exposure of chondrocytes to FGF-2 during 2D expansion and BMP-2 during 3D cultivation. J Cell Biochem 83, 121, 2001.
5. Yang, K.G., Saris, D.B., Geuze, R.E., Helm, Y.J., Rijen, M.H., Verbout, A.J., Dhert, W.J., and Creemers, L.B. Impact of expansion and redifferentiation conditions on chondrogenic capacity of cultured chondrocytes. Tissue Eng 12, 2435, 2006.
6. Claus, S., Aubert-Foucher, E., Demoor, M., Camuzeaux, B., Paumier, A., Piperno, M., Damour, O., Duterque-Coquillaud, M., Galera, P., and Mallein-Gerin, F. Chronic exposure of bone morphogenetic protein-2 favors chondrogenic expression in human articular chondrocytes amplified in monolayer cultures. J Cell Biochem 111, 1642, 2010.
7. Hautier, A., Salentey, V., Aubert-Foucher, E., Bougault, C., Beauchef, G., Ronziere, M.C., De Sobarnitsky, S., Paumier, A., Galera, P., Piperno, M., Damour, O., and Mallein-Gerin, F. Bone morphogenetic protein-2 stimulates chondrogenic expression in human nasal chondrocytes expanded in vitro. Growth Factors 26, 201, 2008.
8. Liu, G., Kawaguchi, H., Ogasawara, T., Asawa, Y., Kishimoto, J., Takahashi, T., Chung, U.I., Yamaoka, H., Asato, H., Nakamura, K., Takato, T., and Hoshi, K. Optimal combination of soluble factors for tissue engineering of permanent cartilage from cultured human chondrocytes. J Biol Chem 282, 20407, 2007.
9. Freyria, A.M., Courtes, S., and Mallein-Gerin, F. [Differentiation of adult human mesenchymal stem cells: chondrogenic effect of BMP-2]. Pathol Biol (Paris) 56, 326, 2008.
10. Valcourt, U., Gouttenoire, J., Moustakas, A., Herbage, D., and Mallein-Gerin, F. Functions of transforming growth factor-beta family type I receptors and Smad proteins in the hypertrophic maturation and osteoblastic differentiation of chondrocytes. J Biol Chem 277, 33545, 2002.
11. Le Guellec, D., Mallein-Gerin, F., Treilleux, I., Bonaventure, J., Peysson, P., and Herbage, D. Localization of the expression of type I, II and III collagen genes in human normal and hypochondrogenesis cartilage canals. Histochem J 26, 695, 1994.
12. Warman, M., Kimura, T., Muragaki, Y., Castagnola, P., Tamei, H., Iwata, K., and Olsen, B.R. Monoclonal antibodies against two epitopes in the human alpha 1 (IX) collagen chain. Matrix 13, 149, 1993.
13. Lefebvre, V., Huang, W., Harley, V.R., Goodfellow, P.N., and de Crombrugghe, B. SOX9 is a potent activator of the chondrocyte-specific enhancer of the pro alpha1(II) collagen gene. Mol Cell Biol 17, 2336, 1997.
14. Marlovits, S., Hombauer, M., Truppe, M., Vecsei, V., and Schlegel, W. Changes in the ratio of type-I and type-II collagen expression during monolayer culture of human chondrocytes. J Bone Joint Surg Br 86, 286, 2004.
15. McAlinden, A., Johnstone, B., Kollar, J., Kazmi, N., and Hering, T.M. Expression of two novel alternatively spliced COL2A1 isoforms during chondrocyte differentiation. Matrix Biol 27, 254, 2008.
16. Piperno, M., Moyen, B., Carillon, Y., Vignon, E., and Damour, O. Communications orales en sessions paralle'les O.09 Greffe de chondorcytes autologues dans les lésions du cartilage fémoral: l'expérience lyonnaise. Rev RhumEd Fr 72, 905, 2005.
17. Takahashi, T., Ogasawara, T., Kishimoto, J., Liu, G., Asato, H., Nakatsuka, T., Uchinuma, E., Nakamura, K., Kawaguchi, H., Chung, U.I., Takato, T., and Hoshi, K. Synergistic effects of FGF-2 with insulin or IGF-I on the proliferation of human auricular chondrocytes. Cell Transplant 14, 683, 2005.
18. Kamil, S.H., Aminuddin, B.S., Bonassar, L.J., Silva, C.A., Weng, Y., Woda, M., Vacanti, C.A., Eavey, R.D., and Vacanti, M.P. Tissue-engineered human auricular cartilage demonstrates euploidy by flow cytometry. Tissue Eng 8, 85, 2002.
19. Wroblewski, J., and Edwall-Arvidsson, C. Inhibitory effects of basic fibroblast growth factor on chondrocyte differentiation. J Bone Miner Res 10, 735, 1995.
20. Bonaventure, J., Kadhom, N., Cohen-Solal, L., Ng, K.H., Bourguignon, J., Lasselin, C., and Freisinger, P. Reexpression of cartilage-specific genes by dedifferentiated human articular chondrocytes cultured in alginate beads. Exp Cell Res 212, 97, 1994.
21. Murphy, C.L., and Sambanis, A. Effect of oxygen tension and alginate encapsulation on restoration of the differentiated phenotype of passaged chondrocytes. Tissue Eng 7, 791, 2001.
22. Murphy, C.L., and Polak, J.M. Control of human articular chondrocyte differentiation by reduced oxygen tension. J Cell Physiol 199, 451, 2004.
23. Yaeger, P.C., Masi, T.L., de Ortiz, J.L., Binette, F., Tubo, R., and McPherson, J.M. Synergistic action of transforming growth factor-beta and insulin-like growth factor-I induces expression of type II collagen and aggrecan genes in adult human articular chondrocytes. Exp Cell Res 237, 318, 1997.
24. Kumar, D., and Lassar, A.B. The transcriptional activity of Sox9 in chondrocytes is regulated by RhoA signaling and actin polymerization. Mol Cell Biol 29, 4262, 2009.
25. Valcourt, U., Ronziere, M.C., Winkler, P., Rosen, V., Herbage, D., and Mallein-Gerin, F. Different effects of bone morphogenetic proteins 2, 4, 12, and 13 on the expression of cartilage and bone markers in the MC615 chondrocyte cell line. Exp Cell Res 251, 264, 1999.
26. Martin, I., Jakob, M., Schafer, D., Dick, W., Spagnoli, G., and Heberer, M. Quantitative analysis of gene expression in human articular cartilage from normal and osteoarthritic joints. Osteoarthritis Cartilage 9, 112, 2001.
27. Park, Y., Sugimoto, M., Watrin, A., Chiquet, M., and Hunziker, E.B. BMP-2 induces the expression of chondrocytespecific genes in bovine synovium-derived progenitor cells cultured in three-dimensional alginate hydrogel. Osteoarthritis Cartilage 13, 527, 2005.