Fibroblast polarization is a matrix-rigidity-dependent process controlled by focal adhesion mechanosensing

Nature Cell Biology

Volumn 13, Issue 12, 2011, Pages 1457-1465

  Prager Khoutorsky, Masha ^a,b,c,d   Lichtenstein, Alexandra ^a,b,c   Krishnan, Ramaswamy ^b,d   Rajendran, Kavitha ^b,d   Mayo, Avi ^a   Kam, Zvi ^a   Geiger, Benjamin ^a,e   Bershadsky, Alexander D ^a,c,e  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^b HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^d MCGILL UNIVERSITY   (Canada)

^e HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIMETICONE; POLYACRYLAMIDE GEL; PROTEIN TYROSINE KINASE; SMALL INTERFERING RNA; 3 (PYRIDIN 3 YL)ACRYLALDEHYDE; 3-(PYRIDIN-3-YL)ACRYLALDEHYDE; ACROLEIN; DRUG DERIVATIVE; GLASS; NYLON; POLY(DIMETHYLSILOXANE) POLYAMIDE COPOLYMER; POLY(DIMETHYLSILOXANE)-POLYAMIDE COPOLYMER; PYRIDINE DERIVATIVE;

ARTICLE; CELL ACTIVITY; CELL ADHESION; CELL ELONGATION; CELL FUNCTION; CELL INTERACTION; CELL POLARITY; CELL SPREADING; CELL STRUCTURE; CELL SURFACE; CELL TRACTION FORCE; CELLULAR PARAMETERS; CONTROLLED STUDY; EXTRACELLULAR MATRIX; FIBROBLAST CULTURE; FOCAL ADHESION; GENE IDENTIFICATION; GENE SILENCING; HUMAN; HUMAN CELL; MECHANOSENSING; PRIORITY JOURNAL; SURFACE PROPERTY; YOUNG MODULUS; CELL CULTURE; CHEMISTRY; CYTOLOGY; FIBROBLAST; GENETICS; MECHANICAL STRESS; MECHANOTRANSDUCTION; METABOLISM; PHYSIOLOGY;

ACROLEIN; CELL POLARITY; CELLS, CULTURED; DIMETHYLPOLYSILOXANES; EXTRACELLULAR MATRIX; FIBROBLASTS; FOCAL ADHESIONS; GLASS; HUMANS; MECHANOTRANSDUCTION, CELLULAR; NYLONS; PROTEIN-TYROSINE KINASES; PYRIDINES; RNA, SMALL INTERFERING; STRESS, MECHANICAL;

EID: 84856134600     PISSN: 14657392     EISSN: 14764679     Source Type: Journal    
DOI: 10.1038/ncb2370     Document Type: Article

References (36)

1. Discher, D. E., Janmey, P. & Wang, Y. L. Tissue cells feel and respond to the stiffness of their substrate. Science 310, 1139-1143 (2005). (Pubitemid 41681732)
2. Geiger, B., Spatz, J. P. & Bershadsky, A. D. Environmental sensing through focal adhesions. Nat. Rev. Mol. Cell Biol. 10, 21-33 (2009).
3. Vogel, V. & Sheetz, M. Local force and geometry sensing regulate cell functions. Nat. Rev. Mol. Cell Biol. 7, 265-275 (2006).
4. Guilak, F. et al. Control of stem cell fate by physical interactions with the extracellular matrix. Cell Stem Cell 5, 17-26 (2009).
5. Janmey, P. A. & Miller, R. T. Mechanisms of mechanical signaling in development and disease. J. Cell Sci. 124, 9-18 (2011).
6. Orr, A. W., Helmke, B. P., Blackman, B. R. & Schwartz, M. A. Mechanisms of mechanotransduction. Dev. Cell 10, 11-20 (2006). (Pubitemid 43025673)
7. Vasiliev, J. M. Spreading of non-transformed and transformed cells. Biochim. Biophys. Acta 780, 21-65 (1985).
8. Petrie, R. J., Doyle, A. D. & Yamada, K. M. Random versus directionally persistent cell migration. Nat. Rev. Mol. Cell Biol. 10, 538-549 (2009).
9. Robinson, D. R., Wu, Y. M. & Lin, S. F. The protein tyrosine kinase family of the human genome. Oncogene 19, 5548-5557 (2000).
10. Cramer, L. P. Forming the cell rear rst: breaking cell symmetry to trigger directed cell migration. Nat. Cell Biol. 12, 628-632 (2010).
11. Brunton, V. G., MacPherson, I. R. & Frame, M. C. Cell adhesion receptors, tyrosine kinases and actin modulators: a complex three-way circuitry. Biochim. Biophys. Acta 1692, 121-144 (2004). (Pubitemid 38891770)
12. Giannone, G. & Sheetz, M. P. Substrate rigidity and force de-ne form through tyrosine phos phatase and kinase pathways. Trends Cell Biol. 16, 213-223 (2006).
13. Sawada, Y. et al. Force sensing by mechanical extension of the Src family kinase substrate p130Cas. Cell 127, 1015-1026 (2006). (Pubitemid 44803062)
14. Butler, J. P., Tolic-Norrelykke, I. M., Fabry, B. & Fredberg, J. J. Traction -elds, moments, and strain energy that cells exert on their surroundings. Am. J. Phys. Cell Phys. 282, C595-C605 (2002). (Pubitemid 34663176)
15. Mitrossilis, D. et al. Single-cell response to stiffness exhibits muscle-like behavior. Proc. Natl Acad. Sci. USA 106, 18243-18248 (2009).
16. Saez, A. et al. Traction forces exerted by epithelial cell sheets. J. Phys. Condens. Matter 22, 194119 (2010).
17. Saez, A., Buguin, A., Silberzan, P. & Ladoux, B. Is the mechanical activity of epithelial cells controlled by deformations or forces? Biophys. J. 89, L52-L54 (2005). (Pubitemid 41725585)
18. Bershadsky, A. D., Balaban, N. Q. & Geiger, B. Adhesion-dependent cell mechanosensitivity. Annu. Rev. Cell Dev. Biol. 19, 677-695 (2003). (Pubitemid 37487365)
19. Puklin-Faucher, E. & Sheetz, M. P. The mechanical integrin cycle. J. Cell Sci. 122, 179-186 (2009).
20. Schwartz, M. A. & DeSimone, D. W. Cell adhesion receptors in mechanotransduction. Curr. Opin. Cell Biol. 20, 551-556 (2008).
21. Hoffman, B. D., Grashoff, C. & Schwartz, M. A. Dynamic molecular processes mediate cellular mechanotransduction. Nature 475, 316-323 (2011).
22. Gof-n, J. M. et al. Focal adhesion size controls tension-dependent recruitment of alpha-smooth muscle actin to stress -bers. J. Cell Biol. 172, 259-268 (2006).
23. Stricker, J., Aratyn-Schaus, Y., Oakes, P. W. & Gardel, M. L. Spatiotemporal constraints on the force-dependent growth of focal adhesions. Biophys. J. 100, 2883-2893 (2011).
24. Fouchard, J., Mitrossilis, D. & Asnacios, A. Acto-myosin based response to stiffness and rigidity sensing. Cell. Adh. Migr. 5, 16-19 (2011).
25. Mitrossilis, D. et al. Real-time single-cell response to stiffness. Proc. Natl Acad. Sci. USA 107, 16518-16523 (2010).
26. Walcott, S. & Sun, S. X. A mechanical model of actin stress -ber formation and substrate elasticity sensing in adherent cells. Proc. Natl Acad. Sci. USA 107, 7757-7762 (2010).
27. Webster, K. D., Crow, A. & Fletcher, D. A. An AFM-based stiffness clamp for dynamic control of rigidity. PLoS One 6, e17807 (2011).
28. Montgomery, D. C. & Runger, G. C. Applied Statistics and Probability for Engineers 2nd edn (John Wiley, 1999).
29. Alexandrova, A. Y. et al. Comparative dynamics of retrograde actin -ow and focal adhesions: formation of nascent adhesions triggers transition from fast to slow -ow. PLoS One 3, e3234 (2008).
30. Choi, C. K. et al. Actin and-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner. Nat. Cell Biol. 10, 1039-1050 (2008).
31. Winograd-Katz, S. E., Itzkovitz, S., Kam, Z. & Geiger, B. Multiparametric analysis of focal adhesion formation by RNAi-mediated gene knockdown. J. Cell Biol. 186, 423-436 (2009).
32. Butler, J. P., Tolic-Norrelykke, I. M., Fabry, B. & Fredberg, J. J. Traction -elds, moments, and strain energy that cells exert on their surroundings. Am. J. Phys. Cell Phys. 282, C595-C605 (2002). (Pubitemid 34663176)
33. Kam, Z., Zamir, E. & Geiger, B. Probing molecular processes in live cells by quantitative multidimensional microscopy. Trends Cell Biol. 11, 329-334 (2001). (Pubitemid 32728173)
34. Zamir, E. et al. Molecular diversity of cell-matrix adhesions. J. Cell Sci. 112 (Pt 11), 1655-1669 (1999). (Pubitemid 29295043)
35. Beeler, J. F., LaRochelle, W. J., Chedid, M., Tronick, S. R. & Aaronson, S. A. Prokaryotic expression cloning of a novel human tyrosine kinase. Mol. Cell Biol. 14, 982-988 (1994). (Pubitemid 24036548)
36. Vartiainen, M., Ojala, P. J., Auvinen, P., Peranen, J. & Lappalainen, P. Mouse A6/twin-lin is an actin monomer-binding protein that localizes to the regions of rapid actin dynamics. Mol. Cell Biol. 20, 1772-1783 (2000). (Pubitemid 30100195)