Real-time single-cell response to stiffness

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 38, 2010, Pages 16518-16523

  Mitrossilis, Démosthène ^a   Fouchard, Jonathan ^a   Pereira, David ^a   Postic, François ^a   Richert, Alain ^a   Saint Jean, Michel ^a   Asnacios, Atef ^a  

^a UNIVERSITÉ PARIS DIDEROT   (France)

Author keywords

Cell mechanics; Dynamic stiffness control; Mechanotransduction

Indexed keywords

ANIMAL CELL; ARTICLE; CELL ACTIVITY; CELL ADHESION; COMPLEX FORMATION; CONTROLLED STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL; RIGIDITY; STATIC ELECTRICITY; ANIMAL; BIOPHYSICS; CELL LINE; ELASTICITY; EXTRACELLULAR MATRIX; MECHANICAL STRESS; MECHANOTRANSDUCTION; PHYSIOLOGY; SIGNAL TRANSDUCTION; SKELETAL MUSCLE;

FIBRONECTIN;

ANIMALS; BIOPHYSICAL PHENOMENA; CELL ADHESION; CELL LINE; ELASTICITY; EXTRACELLULAR MATRIX; FIBRONECTINS; MECHANOTRANSDUCTION, CELLULAR; MICE; MUSCLE FIBERS, SKELETAL; SIGNAL TRANSDUCTION; STATIC ELECTRICITY; STRESS, MECHANICAL;

EID: 78049285759     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1007940107     Document Type: Article

References (34)

1. Discher D, Janmey P, Wang Y (2005) Tissue cells feel and respond to the stiffness of their substrate. Science 310:1139-1143.
2. Vogel V, SheetzM(2006) Local force and geometry sensing regulate cell functions. Nat Rev Mol Cell Biol 7:265-275.
3. Discher D, Mooney D, Zandstra P (2009) Growth factors, matrices, and forces combine and control stem cells. Science 324:1673-1677.
4. Engler A, et al. (2004) Substrate compliance versus ligand density in cell on gel responses. Biophys J 86:617-628.
5. Solon J, Levental I, Sengupta K, Georges P, Janmey P (2007) Fibroblast adaptation and stiffness matching to soft elastic substrates. Biophys J 93:4453-4461.
6. Saez A, Ghibaudo M, Buguin A, Silberzan P, Ladoux B (2007) Rigidity-driven growth and migration of epithelial cells on microstructured anisotropic substrates. Proc Natl Acad Sci USA 104:8281-8286.
7. Allioux-Guerin M, et al. (2009) Spatio-temporal analysis of cell response to a rigidity gradient: A quantitative study by multiple optical tweezers. Biophys J 96:238-247.
8. Mitrossilis D, et al. (2009) Single-cell response to stiffness exhibits muscle-like behavior. Proc Natl Acad Sci USA 106:18243-18248.
9. Lo C, Wang H, Dembo M, Wang Y (2000) Cell movement is guided by the rigidity of the substrate. Biophys J 79:144-152.
10. Engler A, et al. (2004) Myotubes differentiate optimally on substrates with tissue-like stiffness: Pathological implications for soft or stiff microenvironments. J Cell Biol 166:877-887.
11. Engler A, Sen S, Sweeney H, Discher D (2006) Matrix elasticity directs stem cell lineage specification. Cell 126:677-689.
12. Riveline D, et al. (2001) Focal contacts as mechanosensors: Externally applied local mechanical force induces growth of focal contacts by an mDia-dependent and ROCK-independent mechanism. J Cell Biol 153:1175-1186.
13. Balaban N, et al. (2001) Force and focal adhesion assembly: A close relationship studied using elastic micropatterned substrates. Nat Cell Biol 3:466-472.
14. Ingber D (1991) Integrins as mechanochemical transducers. Curr Opin Cell Biol 3:841-848.
15. Wang N, Butler J, Ingber D (1993) Mechanotransduction across the cell surface and through the cytoskeleton. Science 260:1124-1127.
16. Tamada M, Sheetz M, Sawada Y (2004) Activation of a signaling cascade by cytoskeleton stretch. Dev Cell 7:709-718.
17. Kostic A, Sheetz M (2006) Fibronectin rigidity response through Fyn and p130Cas recruitment to the leading edge. Mol Biol Cell 17:2684-2695.
18. Desprat N, Guiroy A, Asnacios A (2006) Microplates-based rheometer for a single living cell. Rev Sci Instrum 77:055111.
19. Desprat N, Richert A, Simeon J, Asnacios A (2005) Creep function of a single living cell. Biophys J 88:2224-2233.
20. Thoumine O, Ott A (1997) Time scale dependent viscoelastic and contractile regimes in fibroblasts probed by microplate manipulation. J Cell Sci 110:2109-2116.
21. Mitrossilis D, Browaeys J, Asnacios A (2009) Method for determining the response of a contractile or expansible biological material. European patent EP09305991.
22. Geiger B, Bershadsky A (2001) Assembly and mechanosensory function of focal contacts. Curr Opin Cell Biol 13:584-592.
23. Bershadsky A, Balaban N, Geiger B (2003) Adhesion-dependent cell mechanosensitivity. Annu Rev Cell Dev Biol 19:677-695.
24. Giannone G, Sheetz M (2006) Substrate rigidity and force define form through tyrosine phosphatase and kinase pathways. Trends Cell Biol 16:213-223.
25. YoshigiM, Hoffman L, Jensen C, Yost M, BeckerleM(2005) Mechanical force mobilizes zyxin from focal adhesions to actin filaments and regulates cytoskeletal reinforcement. J Cell Biol 171:209-215.
26. Lele T, et al. (2006) Mechanical forces alter zyxin unbinding kinetics within focal adhesions of living cells. J Cell Physiol 207:187-194.
27. Na S, et al. (2008) Rapid signal transduction in living cells is a unique feature of mechanotransduction. Proc Natl Acad Sci USA 105:6626-6631.
28. Saez A, Buguin A, Silberzan P, Ladoux B (2005) Is the mechanical activity of epithelial cells controlled by deformations or forces? Biophys J 89:52-54.
29. Mizuno D, Bacabac R, Tardin C, Head D, Schmidt C (2009) High-resolution probing of cellular force transmission. Phys Rev Lett 102:168102.
30. Fouchard J, Mitrossilis D, Asnacios A (2010) Acto-myosin based response to stiffness and rigidity sensing. Cell Adhes Migr 5, in press.
31. Ingber D (2003) Mechanosensation through integrins: Cells act locally but think globally. Proc Natl Acad Sci USA 100:1472-1474.
32. Hamant O, et al. (2008) Developmental patterning by mechanical signals in Arabidopsis. Science 322:1650-1655.
33. Wozniak M, Chen C (2009) Mechanotransduction in development: A growing role for contractility. Nat Rev Mol Cell Bio 10:34-43.
34. Simmons C, et al. (2003) Cyclic strain enhances matrix mineralization by adult human mesenchymal stem cells via the extracellular signal-regulated kinase (ERK1/2) signaling pathway. J Biomech 36:1087-1096.