Split-intein mediated re-assembly of genetically encoded Ca2+ indicators

Cell Calcium

Volumn 51, Issue 1, 2012, Pages 57-64

  Wong, Stanley S C ^a   Kotera, Ippei ^b   Mills, Evan ^a   Suzuki, Hiroshi ^b,c   Truong, Kevin ^a,d  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c UNIVERSITY OF TORONTO   (Canada)

^d UNIVERSITY OF TORONTO   (Canada)

Author keywords

C. elegans; GCaMP2; Genetically encoded calcium indicators; Intein; TN XL

Indexed keywords

CALCIUM ION; INTEIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BIOSENSOR; CAENORHABDITIS ELEGANS; CALCIUM SIGNALING; CELL TYPE; CONTROLLED STUDY; GENETIC CODE; MOLECULAR DYNAMICS; MOLECULAR MODEL; NONHUMAN; NOSTOC; PHARYNGEAL MUSCLE; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PROCESSING; PROTEIN STRUCTURE; STOICHIOMETRY; TRANSGENIC ANIMAL;

CAENORHABDITIS ELEGANS; NOSTOC PUNCTIFORME;

EID: 84856121254     PISSN: 01434160     EISSN: 15321991     Source Type: Journal    
DOI: 10.1016/j.ceca.2011.10.006     Document Type: Article

References (36)

1. Tsien R.W. Calcium channels, stores, and oscillations. Annual Review of Cell Biology 1990, 6:715-760.
2. Clapham D.E. Calcium signaling. Cell 1995, 80:259-268.
3. Berridge M.J., Bootman M.D., Lipp P. Calcium - a life and death signal. Nature 1998, 395:645-648.
4. Berridge M.J., Bootman M.D., Roderick H.L. Calcium signalling: dynamics, homeostasis and remodelling. Nature Reviews Molecular Cell Biology 2003, 4:517-529.
5. Mank M., Griesbeck O. Genetically encoded calcium indicators. Chemical Reviews 2008, 108(5):1550-1564.
6. Rudolf R., et al. Looking forward to seeing calcium. Nature Reviews Molecular Cell Biology 2003, 4:579-586.
7. Tsien R.Y. Imagining imaging's future. Nature Reviews Molecular Cell Biology 2003, 4:SS16-SS21.
8. 2+ indicators: using genetics and molecular design to understand complex physiology. Journal of Physiology 2007, 578(1):55-67.
9. Tian L., et al. Imaging neural activity in worms: flies and mice with improved GCaMP calcium indicators. Nature Methods 2009, 6(12):875-881.
10. Baird G.S., Zacharias D.A., Tsien R.Y. Circular permutation and receptor insertion within green fluorescent proteins. Proceedings of the National Academy of Sciences of the United States of America 1999, 96:11241-11246.
11. 2+ based on green fluorescent proteins and calmodulin. Nature 1997, 388:882-887.
12. 2+ indicator GCaMP2. Proceedings of the National Academy of Sciences of the United States of America 2006, 103(12):4753-4758.
13. Zheng C., Baum B.J. Evaluation of promoters for use in tissue-specific gene delivery. Methods in Molecular Biology 2008, 434:205-219.
14. Ghosh I., Hamilton A.D., Regan L. Antiparallel leucine zipper-directed protein reassembly: application to the green fluorescent protein. Journal of American Chemical Society 2000, 122:5658-5659.
15. Tsien R.Y. The green fluorescent protein. Annual Review of Biochemistry 1998, 67:509-544.
16. Zhang S., Ma C., Chalfie M. Combinatorial marking of cells and organelles with reconstituted fluorescent proteins. Cell 2004, 119(1):137-144.
17. Saleh L., Perler F.B. Protein splicing in cis and in trans. The Chemical Record 2006, 6:183-193.
18. Iwai H., et al. Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiform. FEBS Letters 2006, 580(7):1853-1858.
19. Zettler J., Schutz V., Mootz H.D. The naturally split Npu DnaE intein exhibits an extraordinary high rate in the protein trans-splicing reaction. FEBS Letters 2009, 583(5):909-914.
20. Nagai T., et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nature Biotechnology 2002, 20:87-90.
21. Rizzo M.A., et al. An improved cyan fluorescent protein variant useful for FRET. Nature Biotechnology 2004, 22(4):445-449.
22. Campbell R.E., et al. A monomeric red fluorescent protein. Proceedings of the National Academy of Sciences of the United States of America 2002, 99(12):7877-7882.
23. Mank M., et al. A FRET-based calcium biosensor with fast signal kinetics and high fluorescence change. Biophysical Journal 2006, 90:1790-1796.
24. Lockless S.W., Muir T.W. Traceless protein splicing utilizing evolved split inteins. Proceedings of the National Academy of Sciences of the United States of America 2009, 106(27):10999-11004.
25. y in human epithelial cells. Biochimica et Biophysica Acta: Biomembranes 2009, 1788(5):1176-1187.
26. Jares-Erijman E.A., Jovin T.M. FRET imaging. Nature Biotechnology 2003, 21:1387-1395.
27. Wang Q., et al. Structural basis for calcium sensing by GCaMP2. Structure 2008, 16:1817-1827.
28. Albertson D.G., Thomson J.N. The pharynx of Caenorhabditis elegans. Philosophical Transaction of the Royal Society of London 1975, 275:299-325.
29. Kerr R., et al. Optical imaging of calcium transients in neurons and pharyngeal muscle of C. elegans. Neuron 2000, 26:583-894.
30. 2+ dynamics in pharyngeal muscles in Caenorhabditis elegans during fast pumping. European Molecular Biology Organization 2004, 5(5):521-526.
31. 2+. Proceedings of the National Academy of Sciences of the United States of America 2001, 98(6):3197-3202.
32. Oeemig J.S., et al. Solution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification. FEBS Letters 2009, 538(9):1451-1456.
33. Paulus H. Protein splicing and related forms of protein autoprocessing. Annual Review of Biochemistry 2000, 69:447-496.
34. Anraku Y., Mizutani R., Satow Y. Protein splicing: Its discovery and structural insight into novel chemical mechanisms. IUBMB Life 2005, 57(8):563-574.
35. Truong K., Khorchid A., Ikura M. A Fluorescent cassette-based strategy for engineering multiple domain fusion proteins. BME Biotechnology 2003, 3(1):1-8.
36. Wong S.S.C., Truong K. Fluorescent protein-based methods for on-plate screening of gene insertion. PLoS One 2010, 5(12):1-5.