Transcriptional analysis of human papillomavirus type 16 in histological sections of cervical dysplasia by in situ hybridisation

Journal of Clinical Pathology

Volumn 65, Issue 2, 2012, Pages 164-170

  Coupe, Victoria M ^a   Gonzalez Barreiro L ^a   Gutierrez Berzal J ^a   Melian Boveda A L ^a   Lopez Rodriguez O ^a   Alba Dominguez J ^a   Alba Losada, J ^b  

^a Centre for Molecular Biology   (Spain)

^b Policlinico Lucense   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

DIGOXIGENIN; FORMALDEHYDE; PARAFFIN; RNA; SINGLE STRANDED DNA; VIRUS RNA;

ARTICLE; BIOPSY; CANCER GROWTH; CELLULAR DISTRIBUTION; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC VALUE; DISEASE COURSE; DNA PROBE; GENE EXPRESSION; GENETIC TRANSCRIPTION; HISTOLOGY; HUMAN; HUMAN PAPILLOMAVIRUS TYPE 16; HUMAN TISSUE; IN SITU HYBRIDIZATION; NONHUMAN; ONCOGENE; PRIORITY JOURNAL; START CODON; TISSUE SECTION; UTERINE CERVIX CANCER; UTERINE CERVIX DYSPLASIA; UTERINE CERVIX EPITHELIUM; VIRUS GENE;

BIOPSY; CERVIX UTERI; DNA, VIRAL; FEMALE; GENE EXPRESSION PROFILING; HUMAN PAPILLOMAVIRUS 16; HUMANS; IN SITU HYBRIDIZATION; PAPILLOMAVIRUS INFECTIONS; POLYMERASE CHAIN REACTION; RNA, VIRAL; TRANSCRIPTOME; UTERINE CERVICAL DYSPLASIA;

EID: 84856110511     PISSN: 00219746     EISSN: 14724146     Source Type: Journal    
DOI: 10.1136/jclinpath-2011-200330     Document Type: Article

References (32)

1. Saunier M, Monnier-Benoit S, Mauny F, et al. Analysis of human papillomavirus type 16 (HPV16) DNA load and physical state for identification of HPV16-infected women with high-grade lesions or cervical carcinoma. J Clin Microbiol 2008;46:3678-85.
2. Doorbar J. Molecular biology of human papillomavirus infection and cervical cancer. Clin Sci (Lond) 2006;110:525-41. (Pubitemid 43736116)
3. Kahn JA. HPV vaccination for the prevention of cervical intraepithelial neoplasia. N Engl J Med 2009;361:271-8.
4. Gao P, Zheng J. High-risk HPV E5-induced cell fusion: a critical initiating event in the early stage of HPV-associated cervical cancer. Virol J 2010;7:238.
5. Middleton K, Peh W, Southern S, et al. Organization of human papillomavirus productive cycle during neoplastic progression provides a basis for selection of diagnostic markers. J Virol 2003;77:10186-201. (Pubitemid 37129663)
6. Bechtold V, Beard P, Raj K. Human papillomavirus type 16 E2 protein has no effect on transcription from episomal viral DNA. J Virol 2003;77: 2021-8. (Pubitemid 36110014)
7. Xue Y, Bellanger S, Zhang W, et al. HPV16 E2 is an immediate early marker of viral infection, preceding E7 expression in precursor structures of cervical carcinoma. Cancer Res 2010;70:5316-25.
8. Scheffner M, Werness BA, Huibregtse JM, et al. The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53. Cell 1990;63:1129-36. (Pubitemid 120035062)
9. Sherman L, Jackman A, Itzhaki H, et al. Inhibition of serum- and calcium-induced differentiation of human keratinocytes by HPV16 E6 oncoprotein: role of p53 inactivation. Virology 1997;237:296-306. (Pubitemid 27489268)
10. Giarre M, Caldeira S, Malanchi I, et al. Induction of pRb degradation by the human papillomavirus type 16 E7 Protein is essential to efficiently overcome p16INK4a-imposed G1 cell cycle arrest. J Virol 2001;75:4705-12. (Pubitemid 32381513)
11. Boyer SN, Wazer DE, Band V. E7 protein of human papilloma virus-16 induces degradation of retinoblastoma protein through the ubiquitin-proteasome pathway. Cancer Res 1996;56:4620-4. (Pubitemid 26338060)
12. Bodaghi S, Jia R, Zheng ZM. Human papillomavirus type 16 E2 and E6 are RNA-binding proteins and inhibit in vitro splicing of pre-mRNAs with suboptimal splice sites. Virology 2009;386:32-43.
13. del Moral-Hernández O, López-Urrutia E, Bonilla-Moreno R, et al. The HPV-16 E7 oncoprotein is expressed mainly from the unspliced E6/E7 transcript in cervical carcinoma C33-A cells. Arch Virol 2010;155:1959-70.
14. Mole S, McFarlane M, Chuen-Im T, et al. RNA splicing factors regulated by HPV16 during cervical tumour progression. J Pathol 2009;219:383-91.
15. Zheng ZM. Viral oncogenes, noncoding RNAs, and RNA splicing in human tumor viruses. Int J Biol Sci 2010;6:730-55.
16. Nakahara T, Peh WL, Doorbar J, et al. Human papillomavirus type 16 E1circumflexE4 contributes to multiple facets of the papillomavirus life cycle. J Virol 2005;79:13150-65. (Pubitemid 41433233)
17. Klaes R, Friedrich T, Spitkovsky D, et al. Overexpression of p16INK4A as a specific marker for dysplastic and neoplastic epithelial cells of the cervix uteri. Int J Cancer 2001;92:276-84. (Pubitemid 32225381)
18. Sambrook J, Russel D. Molecular Cloning A Laboratory Manual. 3rd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, 2001;1:6.23-6.7.
19. Kerstens HM, Poddighe PJ, Hanselaar AG. A novel in situ hybridization signal amplification method based on the deposition of biotinylated tyramine. J Histochem Cytochem 1995;43:347-52.
20. Evans MF, Aliesky HA, Cooper K. Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens. BMC Clin Pathol 2003;3:2.
21. Evans MF, Adamson CS, Simmons-Arnold L, et al. Touchdown General Primer (GP5 +/GP6+) PCR and optimized sample DNA concentration support the sensitive detection of human papillomavirus. BMC Clin Pathol 2005;5:10.
22. Zheng ZM, Baker CC. Papillomavirus genome structure, expression, and posttranscriptional regulation. Front Biosci 2006;11:2286-302. (Pubitemid 43822560)
23. Evans MF, Mount SL, Beatty BG, et al. Biotinyl-tyramide-based in situ hybridization signal patterns distinguish human papillomavirus type and grade of cervical intraepithelial neoplasia. Mod Pathol 2002;15:1339-47. (Pubitemid 35463003)
24. Seedorf K, Krammer G, Durst M, et al. Human papillomavirus type 16 DNA sequence. Virology 1985;145:181-5. (Pubitemid 15234421)
25. Romanczuk H, Howley PM. Disruption of either the E1 or the E2 regulatory gene of human papillomavirus type 16 increases viral immortalization capacity. Proc Natl Acad Sci U S A 1992;89:3159-63.
26. Stoler MH, Rhodes CR, Whitbeck A, et al. Human papillomavirus type 16 and 18 gene expression in cervical neoplasias. Hum Pathol 1992;23:117-28.
27. Hu L, Plafker K, Vorozhko V, et al. Human papillomavirus 16 E5 induces bi-nucleated cell formation by cell-cell fusion. Virology 2009;384:125-34.
28. Auvinen E, Kujari H, Arstila P, et al. Expression of the L2 and E7 genes of the human papillomavirus type 16 in female genital dysplasias. Am J Pathol 1992;141:1217-24.
29. Steger G, Olszewsky M, Stockfleth E, et al. Prevalence of antibodies to human papillomavirus type 8 in human sera. J Virol 1990;64:4399-406. (Pubitemid 20279851)
30. Lace MJ, Anson JR, Thomas GS, et al. The E8-E2 gene product of human papillomavirus type 16 represses early transcription and replication but is dispensable for viral plasmid persistence in keratinocytes. J Virol 2008;82:10841-53. (Pubitemid 352691200)
31. Fertey J, Ammermann I, Winkler M, et al. Interaction of the papillomavirus E8dE2C protein with the cellular CHD6 protein contributes to transcriptional repression. J Virol 2010;84:9505-15.
32. Poljak M, Kocjan BJ. Commercially available assays for multiplex detection of alpha human papillomaviruses. Expert Rev Anti Infect Ther 2010;8:1139-62.