Tandem repeat markers as novel diagnostic tools for high resolution fingerprinting of Wolbachia

BMC Microbiology

Volumn 12, Issue SUPPL. 1, 2012, Pages

  Riegler, Markus ^a   Iturbe Ormaetxe, Iñaki ^b,d   Woolfit, Megan ^b,d   Miller, Wolfgang J ^c   O'Neill, Scott L ^b,d  

^a WESTERN SYDNEY UNIVERSITY   (Australia)

^b UNIVERSITY OF QUEENSLAND   (Australia)

^c MEDICAL UNIVERSITY OF VIENNA   (Austria)

^d MONASH UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ANKYRIN; DNA; BACTERIAL DNA; GENETIC MARKER;

ALLELE; ANIMAL EXPERIMENT; ARTICLE; BACTERIAL GENOME; BACTERIUM ISOLATION; COMPUTER MODEL; CONTROLLED STUDY; DIAGNOSTIC VALUE; DNA EXTRACTION; DNA FINGERPRINTING; DROSOPHILA MELANOGASTER; GENE AMPLIFICATION; GENE SEQUENCE; GENETIC POLYMORPHISM; GENETIC VARIABILITY; MULTILOCUS SEQUENCE TYPING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; TANDEM REPEAT; VARIABLE NUMBER OF TANDEM REPEAT; WOLBACHIA; ANIMAL; CLASSIFICATION; GENETIC MARKER; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; PHYLOGENY; PROCEDURES;

BACTERIA (MICROORGANISMS); DROSOPHILA MELANOGASTER; INVERTEBRATA; WOLBACHIA; WOLBACHIA PIPIENTIS;

ANIMALS; DNA FINGERPRINTING; DNA, BACTERIAL; DROSOPHILA MELANOGASTER; GENETIC MARKERS; GENETIC VARIATION; GENOME, BACTERIAL; PHYLOGENY; TANDEM REPEAT SEQUENCES; WOLBACHIA;

EID: 84856006676     PISSN: None     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-12-S1-S12     Document Type: Article

References (82)

1. Distribution of Wolbachia among neotropical arthropods. Werren JH, Windsor D, Guo LR, Proceedings of the Royal Society of London Series B-Biological Sciences 1995 262 1364 197 204 10.1098/rspb.1995.0196 (Pubitemid 26452508)
2. Wolbachia in two insect host-parasitoid communities. West SA, Cook JM, Werren JH, Godfray HCJ, Molecular Ecology 1998 7 11 1457 1465 10.1046/j.1365-294x.1998.00467.x 9819901 (Pubitemid 28504063)
3. Long PCR improves Wolbachia DNA amplification: wsp sequences found in 76% of sixty-three arthropod species. Jeyaprakash A, Hoy MA, Insect Molecular Biology 2000 9 4 393 405 10.1046/j.1365-2583.2000.00203.x 10971717
4. How many species are infected with Wolbachia?-A statistical analysis of current data. Hilgenboecker K, Hammerstein P, Schlattmann P, Telschow A, Werren JH, FEMS Microbiology Letters 2008 281 2 215 220 10.1111/j.1574-6968.2008.01110. x 18312577 (Pubitemid 351422951)
5. Evidence for low-titre infections in insect symbiosis: Wolbachia in the bark beetle Pityogenes chalcographus (Coleoptera, Scolytinae). Arthofer W, Riegler M, Avtzis DN, Stauffer C, Environmental Microbiology 2009 11 8 1923 1933 10.1111/j.1462-2920.2009.01914.x 19383035
6. Influential passengers. Inherited microorganisms and arthropod reproduction. O'Neill SL, Hoffmann AA, Werren JH, Oxford: Oxford University Press 1997
7. Wolbachia pipientis: microbial manipulator of arthropod reproduction. Stouthamer R, Breeuwer JAJ, Hurst GDD, Annual Review of Microbiology 1999 53 71 102 10.1146/annurev.micro.53.1.71 10547686 (Pubitemid 29503726)
8. Wolbachia: Master manipulators of invertebrate biology. Werren JH, Baldo L, Clark ME, Nature Reviews Microbiology 2008 6 10 741 751 10.1038/nrmicro1969 18794912
9. Arthropods shopping for Wolbachia. Schneider D, Miller WJ, Riegler M, Manipulative Tenants Bacteria associated with arthropods Boca Raton: CRC Press, Zchori-Fein E, Bourtzis K, 2011 149 173
10. Wolbachia bacterial endosymbionts of filarial nematodes. Taylor MJ, Bandi C, Hoerauf A, Adv Parasitol 2005 60 245 284 16230105 (Pubitemid 41447520)
11. Evolution and phylogeny of Wolbachia-reproductive parasites of arthropods. Werren JH, Zhang W, Guo LR, Proceedings of the Royal Society of London Series B-Biological Sciences 1995 261 1360 55 63 10.1098/rspb.1995.0117 (Pubitemid 26447296)
12. Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences. Zhou WG, Rousset F, O'Neill S, Proceedings of the Royal Society of London Series B-Biological Sciences 1998 265 1395 509 515 10.1098/rspb.1998.0324 (Pubitemid 28140306)
13. Taxonomic status of the intracellular bacterium Wolbachia pipientis. Lo N, Paraskevopoulos C, Bourtzis K, O'Neill SL, Werren JH, Bordenstein SR, Bandi C, International Journal of Systematic and Evolutionary Microbiology 2007 57 3 654 657 10.1099/ijs.0.64515-0 17329802 (Pubitemid 46442146)
14. Revisiting Wolbachia supergroup typing based on wsp: spurious lineages and discordance in MLST. Baldo L, Werren JH, Current Microbiology 2007 55 1 81 87 10.1007/s00284-007-0055-8 17551786 (Pubitemid 46945353)
15. How diverse is the genus Wolbachia? Multiple-gene sequencing reveals a putatively new Wolbachia supergroup recovered from spider mites (Acari: Tetranychidae). Ros VID, Fleming VM, Feil EJ, Breeuwer JAJ, Applied and Environmental Microbiology 2009 75 4 1036 1043 10.1128/AEM.01109-08 19098217
16. 16S ribosomal RNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. O'Neill SL, Giordano R, Colbert AME, Karr TL, Robertson HM, Proceedings of the National Academy of Sciences of the United States of America 1992 89 7 2699 2702 10.1073/pnas.89.7.2699 1557375
17. Recombination confounds interpretations of Wolbachia evolution. Jiggins FM, von der Schulenburg JHG, Hurst GDD, Majerus MEN, Proceedings of the Royal Society of London Series B-Biological Sciences 2001 268 1474 1423 1427 10.1098/rspb.2001.1656 (Pubitemid 32625609)
18. The rate of recombination in Wolbachia bacteria. Jiggins FM, Molecular Biology and Evolution 2002 19 9 1640 1643 12200493
19. Widespread recombination throughout Wolbachia genomes. Baldo L, Bordenstein S, Wernegreen JJ, Werren JH, Molecular Biology and Evolution 2006 23 2 437 449 16267140 (Pubitemid 43100057)
20. Toward a Wolbachia multilocus sequence typing system: Discrimination of Wolbachia strains present in Drosophila species. Paraskevopoulos C, Bordenstein SR, Wernegreen JJ, Werren JH, Bourtzis K, Current Microbiology 2006 53 5 388 395 10.1007/s00284-006-0054-1 17036209 (Pubitemid 44673733)
21. Multilocus sequence typing system for the endosymbiont Wolbachia pipientis. Baldo L, Hotopp JCD, Jolley KA, Bordenstein SR, Biber SA, Choudhury RR, Hayashi C, Maiden MCJ, Tettelin H, Werren JH, Applied and Environmental Microbiology 2006 72 11 7098 7110 10.1128/AEM.00731-06 16936055 (Pubitemid 44748423)
22. Insight into the routes of Wolbachia invasion: High levels of horizontal transfer in the spider genus Agelenopsis revealed by Wolbachia strain and mitochondrial DNA diversity. Baldo L, Ayoub NA, Hayashi CY, Russell JA, Stahlhut JK, Werren JH, Molecular Ecology 2008 17 2 557 569 18179432
23. Multiple rescue factors within a Wolbachia strain. Zabalou S, Apostolaki A, Pattas S, Veneti Z, Paraskevopoulos C, Livadaras I, Markakis G, Brissac T, Merçot H, Bourtzis K, Genetics 2008 178 4 2145 2160 10.1534/genetics.107. 086488 18430940
24. Extensive genomic diversity of closely related Wolbachia strains. Ishmael N, Hotopp JCD, Loanidis P, Biber S, Sakamoto J, Siozios S, Nene V, Werren J, Boutriz K, Bordenstein SR, et al. Microbiology 2009 155 7 2211 2222 10.1099/mic.0.027581-0 19389774
25. Naturally-occurring Wolbachia infection in Drosophila simulans that does not cause cytoplasmic incompatibility. Hoffmann AA, Clancy D, Duncan J, Heredity 1996 76 1 8 10.1038/hdy.1996.1 8575931
26. Wolbachia, normally a symbiont of Drosophila, can be virulent, causing degeneration and early death. Min KT, Benzer S, Proceedings of the National Academy of Sciences of the United States of America 1997 94 20 10792 10796 10.1073/pnas.94.20.10792 9380712 (Pubitemid 27430813)
27. Wolbachia density and virulence attenuation after transfer into a novel host. McGraw EA, Merritt DJ, Droller JN, O'Neill SL, Proceedings of the National Academy of Sciences of the United States of America 2002 99 5 2918 2923 10.1073/pnas.052466499 11880639 (Pubitemid 34240561)
28. Stable introduction of a life-shortening Wolbachia infection into the mosquito Aedes aegypti. McMeniman CJ, Lane RV, Cass BN, Fong AWC, Sidhu M, Wang YF, O'Neill SL, Science 2009 323 5910 141 144 10.1126/science.1165326 19119237
29. Development of a physical and genetic map of the virulent Wolbachia strain wMelPop. Sun LV, Riegler M, O'Neill SL, Journal of Bacteriology 2003 185 24 7077 7084 10.1128/JB.185.24.7077-7084.2003 14645266 (Pubitemid 37509822)
30. Evidence for a global Wolbachia replacement in Drosophila melanogaster. Riegler M, Sidhu M, Miller WJ, O'Neill SL, Current Biology 2005 15 1428 1433 10.1016/j.cub.2005.06.069 16085497 (Pubitemid 41097549)
31. Microevolution and history of the plague bacillus, Yersinia pestis. Achtman M, Morelli G, Zhu P, Wirth T, Diehl I, Kusecek B, Vogler AJ, Wagner DM, Allender CJ, Easterday WR, et al. Proceedings of the National Academy of Sciences of the United States of America 2004 101 51 17837 17842 10.1073/pnas.0408026101 15598742 (Pubitemid 40051973)
32. Tandem repeats analysis for the high resolution phylogenetic analysis of Yersinia pestis. Pourcel C, André-Mazeaud F, Neubauer H, Ramisse F, Vergnaud G, BMC Microbiology 2004 4
33. Worldwide genetic relationships among Francisella tularensis isolates determined by multiple-locus variable-number tandem repeat analysis. Johansson A, Farlow J, Larsson P, Dukerich M, Chambers E, Byström M, Fox J, Chu M, Forsman M, Sjöstedt A, et al. Journal of Bacteriology 2004 186 17 5808 5818 10.1128/JB.186.17.5808-5818.2004 15317786 (Pubitemid 39128654)
34. Variable number tandem repeat typing of bacteria. Yazdankhah SP, Lindstedt BA, Comparative Genomics Methods in Molecular Biolgy Totowa, NJ: Humana Press, Bergman NH, 2007 396 395 405 (Pubitemid 350190054)
35. Multiple locus variable number of tandem repeats analysis. Vergnaud G, Pourcel C, Methods in molecular biology (Clifton, NJ) 2009 551 141 158 10.1007/978-1-60327-999-4-12
36. Distribution, expression, and motif variability of ankyrin domain genes in Wolbachia pipientis. Iturbe-Ormaetxe I, Burke GR, Riegler M, O'Neill SL, Journal of Bacteriology 2005 187 5136 5145 10.1128/JB.187.15.5136-5145.2005 16030207 (Pubitemid 41022932)
37. Transposable element polymorphism of Wolbachia in the mosquito Culex pipiens: evidence of genetic diversity, superinfection and recombination. Duron O, Lagnel J, Raymond M, Bourtzis K, Fort P, Weill M, Molecular Ecology 2005 14 5 1561 1573 10.1111/j.1365-294X.2005.02495.x 15813794 (Pubitemid 40554811)
38. Evolutionary dynamics of wAu-like Wolbachia variants in neotropical Drosophila spp. Miller WJ, Riegler M, Applied and Environmental Microbiology 2006 72 1 826 835 10.1128/AEM.72.1.826-835.2006 16391124
39. Infectious speciation revisited: Impact of symbiont-depletion on female fitness and mating behavior of drosophila paulistorum. Miller WJ, Ehrman L, Schneider D, PLoS Pathogens 2010 6 12
40. Characterization of an intergenic polymorphic site (pp-hC1A-5) in Wolbachia pipientis (wPip). Petridis M, Chatzidimitriou D, Molecular Ecology Resources 2011 11 4 753 756 10.1111/j.1755-0998.2011.02991.x 21676204
41. Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements. Wu M, Sun LV, Vamathevan J, Riegler M, Deboy R, Brownlie JC, McGraw EA, Martin W, Esser C, Ahmadinejad N, et al. Public Library of Science Biology 2004 2 3 327 341
42. The ankyrin repeat as molecular architecture for protein recognition. Mosavi LK, Cammett TJ, Desrosiers DC, Peng ZY, Protein Science 2004 13 6 1435 1448 10.1110/ps.03554604 15152081 (Pubitemid 38669232)
43. Ankyrin repeat proteins comprise a diverse family of bacterial type IV effectors. Pan X, Lührmann A, Satoh A, Laskowski-Arce MA, Roy CR, Science 2008 320 5883 1651 1654 10.1126/science.1158160 18566289 (Pubitemid 351931257)
44. Infection by Wolbachia: from passengers to residents. Merçot H, Poinsot D, Comptes Rendus-Biologies 2009 332 2-3 284 297 10.1016/j.crvi.2008.09. 010 19281959
45. Unidirectional incompatibility between populations of Drosophila simulans. Hoffmann AA, Turelli M, Simmons GM, Evolution 1986 40 692 701 10.2307/2408456
46. Wolbachia infections and superinfections in cytoplasmically incompatible populations of the European cherry fruit fly Rhagoletis cerasi (Diptera, Tephritidae). Riegler M, Stauffer C, Molecular Ecology 2002 11 11 2425 2434 12406252 (Pubitemid 35301912)
47. Wolbachia-induced cytoplasmic incompatibility as a means for insect pest population control. Zabalou S, Riegler M, Theodorakopoulou M, Stauffer C, Savakis C, Bourtzis K, Proceedings of the National Academy of Sciences of the United States of America 2004 101 15042 15045 10.1073/pnas.0403853101 15469918 (Pubitemid 39391715)
48. Persistence of an extreme sex-ratio bias in natural population. Dyson EA, Hurst GD, Proceedings of the National Academy of Sciences USA 2004 101 6520 6523 10.1073/pnas.0304068101 (Pubitemid 38586006)
49. Molecular evidence for a close relative of the arthropod endosymbiont Wolbachia in a filarial worm. Sironi M, Bandi C, Sacchi L, DiSacco B, Damiani G, Genchi C, Molecular and Biochemical Parasitology 1995 74 2 223 227 10.1016/0166-6851(95)02494-8 8719164 (Pubitemid 26024478)
50. Preparation, molecular weight, base composition and secondary structure of giant nuclear ribonucleic acid. Holmes DS, Bonner J, Biochemistry 1973 12 12 2330 2338 10.1021/bi00736a023 4710584
51. Tandem repeats finder: a program to analyse DNA sequences. Benson G, Nucleic Acid Research 1999 27 2 573 580 10.1093/nar/27.2.573 (Pubitemid 29210025)
52. The mosaic genome structure of the Wolbachia wRi strain infecting Drosophila simulans. Klasson L, Westberg J, Sapountzis P, Näslund K, Lutnaes Y, Darby AC, Veneti Z, Chen L, Braig HR, Garrett R, et al. Proceedings of the National Academy of Sciences of the United States of America 2009 106 14 5725 5730 10.1073/pnas.0810753106 19307581
53. Genome evolution of Wolbachia strain wPip from the Culex pipiens group. Klasson L, Walker T, Sebaihia M, Sanders MJ, Quail MA, Lord A, Sanders S, Earl J, O'Neill SL, Thomson N, et al. Molecular Biology and Evolution 2008 25 9 1877 1887 10.1093/molbev/msn133 18550617
54. The Wolbachia genome of Brugia malayi: endosymbiont evolution within a human pathogenic nematode. Foster J, Ganatra M, Kamal I, Ware J, Makarova K, Ivanova N, Bhattacharyya A, Kapatral V, Kumar S, Posfai J, et al. Public Library of Science Biology 2005 3 4 121
55. The genus Wolbachia. Riegler M, O'Neill SL, The Prokaryotes: A handbook on the biology of bacteria New York: Springer, Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E, 2006 5 547 561 Proteobacteria: Alpha and Beta Subclass
56. DNA strider-an inexpensive sequence analysis package for the Macintosh. Douglas SE, Molecular Biotechnology 1995 3 1 37 45 10.1007/BF02821333 7606503
57. SMART 4.0: towards genomic data integration. Letunic I, Copley RR, Schmidt S, Ciccarelli FD, Doerks T, Schultz J, Ponting CP, Bork P, Nucleic Acids Research 2004 32 DATABASE ISS. 142 D144 14681379
58. SMART, a simple modular architecture research tool: identification of signaling domains. Schultz J, Milpetz F, Bork P, Ponting CP, Proceedings of the National Academy of Sciences of the United States of America 1998 95 11 5857 5864 10.1073/pnas.95.11.5857 9600884 (Pubitemid 28248931)
59. T-coffee: a novel method for fast and accurate multiple sequence alignment. Notredame C, Higgins DG, Heringa J, Journal of Molecular Biology 2000 302 1 205 217 10.1006/jmbi.2000.4042 10964570
60. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Guindon S, Gascuel O, Systematic Biology 2003 52 5 696 704 10.1080/10635150390235520 14530136 (Pubitemid 37365050)
61. Hidden Wolbachia diversity in field populations of the European cherry fruit fly, Rhagoletis cerasi (Diptera, Tephritidae). Arthofer W, Riegler M, Schneider D, Krammer M, Miller WJ, Stauffer C, Molecular Ecology 2009 18 18 3816 3830 10.1111/j.1365-294X.2009.04321.x 19732336
62. Wolbachia transfer from Rhagoletis cerasi to Drosophila simulans: investigating the outcomes of host-symbiont coevolution. Riegler M, Charlat S, Stauffer C, Merçot H, Applied and Environmental Microbiology 2004 70 1 273 279 10.1128/AEM.70.1.273-279.2004 14711652 (Pubitemid 38090214)
63. High frequencies of short frameshifts in poly-CA/TG tandem repeats borne by bacteriophage M13 in Escherichia coli K-12. Levinson G, Gutman GA, Nucleic Acids Research 1987 15 13 5323 5338 10.1093/nar/15.13.5323 3299269
64. Expansions and contractions in a tandem repeat induced by double-strand break repair. Pques F, Leung WY, Haber JE, Molecular and Cellular Biology 1998 18 4 2045 2054 9528777 (Pubitemid 28152654)
65. DNA repeats lead to the accelerated loss of gene order in bacteria. Rocha EPC, Trends in Genetics 2003 19 11 600 603 10.1016/j.tig.2003.09.011 14585609 (Pubitemid 37338377)
66. The genome of the heartwater agent Ehrlichia ruminantium contains multiple tandem repeats of actively variable copy number. Collins NE, Liebenberg J, De Villiers EP, Brayton KA, Louw E, Pretorius A, Faber FE, Van Heerden H, Josemans A, Van Kleef M, et al. Proceedings of the National Academy of Sciences of the United States of America 2005 102 3 838 843 10.1073/pnas.0406633102 15637156 (Pubitemid 40282752)
67. DNA secondary structures and the evolution of hypervariable tandem arrays. Weitzmann MN, Woodford KJ, Usdin K, Journal of Biological Chemistry 1997 272 14 9517 9523 10.1074/jbc.272.14.9517 9083093 (Pubitemid 27154969)
68. Molecular drive: A cohesive mode of species evolution. Dover G, Nature 1982 299 5879 111 117 10.1038/299111a0 7110332
69. A comparative approach to the study of microsatellite evolution. Amos W, Microsatellites Evolution and Applications Oxford: Oxford University Press, Goldstein DB, Schlötterer C, 1999 66 79
70. Male development time influences the strength of Wolbachia-induced cytoplasmic incompatibility expression in Drosophila melanogaster. Yamada R, Floate KD, Riegler M, O'Neill SL, Genetics 2007 177 2 801 808 10.1534/genetics.106.068486 17660578 (Pubitemid 350005633)
71. Ankyrin repeat domain-encoding genes in the wPip strain of Wolbachia from the Culex pipiens group. Walker T, Klasson L, Sebaihia M, Sanders MJ, Thomson NR, Parkhill J, Sinkins SP, BMC Biology 2007 5
72. Wolbachia variability and host effects on crossing type in Culex mosquitoes. Sinkins SP, Walker T, Lynd AR, Steven AR, Makepeace BL, Godfray HC, Parkhill J, Nature 2005 436 257 260 10.1038/nature03629 16015330 (Pubitemid 41021276)
73. Serendipitous discovery of Wolbachia genomes in multiple Drosophila species. Salzberg SL, Hotopp JC, Delcher AL, Pop M, Smith DR, Eisen MB, Nelson WC, Genome Biology 2005 6 3 23 10.1186/gb-2005-6-3-r23 15774024
74. Biology of Wolbachia. Werren JH, Annual Review of Entomology 1997 42 587 609 10.1146/annurev.ento.42.1.587 15012323 (Pubitemid 27169561)
75. Partial cytoplasmic incompatibility between two Australian populations of Drosophila melanogaster. Hoffmann AA, Entomologia Experimentalis Et Applicata 1988 48 61 67 10.1111/j.1570-7458.1988.tb02299.x
76. Male age, host effects and the weak expression or nonexpression of cytoplasmic incompatibility in Drosophila strains infected by maternally transmitted Wolbachia. Reynolds KT, Hoffmann AA, Genetical Research 2002 80 2 79 87 12534211 (Pubitemid 35256424)
77. Natural Wolbachia infections in the Drosophila yakuba species complex do not induce cytoplasmic incompatibility but fully rescue the wRi modification. Zabalou S, Charlat S, Nirgianaki A, Lachaise D, Merçot H, Bourtzis K, Genetics 2004 167 2 827 834 10.1534/genetics.103.015990 15238531 (Pubitemid 38931867)
78. Bidirectional incompatibility between conspecific populations of Drosophila simulans. O'Neill SL, Karr TL, Nature 1990 348 178 180 10.1038/348178a0 2234083 (Pubitemid 20368922)
79. Variability within the Seychelles cytoplasmic incompatibility system in Drosophila simulans. Merçot H, Llorente B, Jacques M, Atlan A, Montchampmoreau C, Genetics 1995 141 3 1015 1023 8582608
80. Wolbachia infections and the expression of cytoplasmic incompatibility in Drosophila sechellia and D. mauritiana. Giordano R, O'Neill SL, Robertson HM, Genetics 1995 140 4 1307 1317 7498772
81. You can't keep a good parasite down: evolution of a male-killer suppressor uncovers cytoplasmic incompatibility. Hornett EA, Duplouy AMR, Davies N, Roderick GK, Wedell N, Hurst GDD, Charlat S, Evolution 2008 62 5 1258 1263 10.1111/j.1558-5646.2008.00353.x 18298644 (Pubitemid 351623940)
82. Functional test of the influence of Wolbachia genes on cytoplasmic incompatibility expression in Drosophila melanogaster. Yamada R, Iturbe-Ormaetxe I, Brownlie JC, O'Neill SL, Insect Molecular Biology 2011 20 1 75 85 10.1111/j.1365-2583.2010.01042.x 20854481