Mammalian cell HPRT gene mutation assay: Test methods

Methods in Molecular Biology

Volumn 817, Issue , 2012, Pages 55-67

  Johnson, George E ^a  

^a SWANSEA UNIVERSITY   (United Kingdom)

Author keywords

HPRT gene mutation; Human cells; Mammalian cell mutation; Mutation testing

Indexed keywords

HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; MUTAGENIC AGENT;

ANIMAL; ARTICLE; CELL LINE; DNA DAMAGE; DRUG EFFECT; GENETICS; HUMAN; METHODOLOGY; MUTAGEN TESTING; MUTATION; STANDARD;

ANIMALS; CELL LINE; DNA DAMAGE; HUMANS; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; MUTAGENICITY TESTS; MUTAGENS; MUTATION;

BACTERIA (MICROORGANISMS); MAMMALIA;

EID: 84855999565     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-421-6_4     Document Type: Article

References (26)

1. DOH (2000) Department of Health Guidance for the testing of chemicals for Mutagenicity. Committee on Mutagenicity of Chemicals in Food, Consumer Products and the Environment.
2. OECD (1997) Organisation for Economic Co-operation and Development. Ninth addendum to the OECD Guidelines for the Testing of Chemicals. In Vitro Mammalian Cell Gene Mutation Test: 476.
3. DeMarini D M, Brockman H E, de Serres F J, Evans H H, Stankowski, Jr L F and Hsie AW (1989) Specific-locus mutations induced in eukaryotes (especially mammalian cells) by radiation and chemicals: a perspective, Mutat Res 220: 11-29. (Pubitemid 19028961)
4. Moore M M, Honma M, Clements J, Awogi T, Bolcsfoldi G, Cole J, Gollapudi B, Harrington-Brock K, Mitchell A, Muster W, Myhr B, O'Donovan M, Ouldelhkim M C, San R, Shimada H and Stankowski, Jr L F (2000) Mouse lymphoma thymidine kinase locus gene mutation assay: International Workshop on Genotoxicity Test Procedures Workgroup Report, Environ Mol Mutagen 35: 185-190. (Pubitemid 30196662)
5. Furth E E, Thilly W G, Penman B W, Liber W L and Rand W M (1981) Quantitative assay for mutation in diploid human lymphoblasts using microtiter plates, Anal Biochem 110: 1-8. (Pubitemid 11157545)
6. Moore M M, Parker L, Huston J, Harrington-Brock K and Dearfield K L (1991) Comparison of mutagenicity results for nine compounds evaluated at the hgprt locus in the standard and suspension CHO assays, Mutagenesis 6: 77-85.
7. Crespi C L, Gonzalez F J, Steimel D T, Turner T R, Gelboin H V, Penman B W and Langenbach R (1991) A metabolically competent human cell line expressing five cDNAs encoding procarcinogen-activating enzymes: application to mutagenicity testing, Chem Res Toxicol 4: 566-572.
8. Crespi C L and Thilly W G (1984) Assay for gene mutation in a human lymphoblast line, AHH-1, competent for xenobiotic metabolism, Mutat Res 128: 221-230. (Pubitemid 14026605)
9. Doak S H (2008) Aneuploidy in upper gastrointestinal tract cancers-a potential prognostic marker? Mutat Res 651: 93-104.
10. Parry J M, Parry E M, Johnson G, Quick E and Waters E M (2005) The detection of genotoxic activity and the quantitative and qualitative assessment of the consequences of exposures, Exp Toxicol Pathol 57: Suppl 1 205-212. (Pubitemid 40884493)
11. Caskey C T and Kruh G D (1979) The HPRT locus, Cell 16: 1-9. (Pubitemid 9097670)
12. Chen T, Harrington-Brock K and Moore M M (2002) Mutant frequency and mutational spectra in the Tk and Hprt genes of N-ethyl-N-nitrosourea-treated mouse lymphoma cells, Environ Mol Mutagen 39: 296-305. (Pubitemid 34742148)
13. Tindall K R and Stankowski Jr L F (1989) Molecular analysis of spontaneous mutations at the gpt locus in Chinese hamster ovary (AS52) cells, Mutation Research/Reviews in Genetic Toxicology 220: 241-253. (Pubitemid 19078875)
14. Tindall K R, Stankowski Jr L F, Machanoff R and Hsie A W (1986) Analyses of mutation in pSV2gpt-transformed CHO cells, Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 160: 121-131. (Pubitemid 16106690)
15. Moore M M, DeMarini D M, DeSerres F J and Tindall K R (1987) Banbury Report 28: Mammalian Cell Mutagenesis, in: N.Y. Cold Sprind Harbor Laboratory, New York (Ed.).
16. Tates A D, van Dam F J, de Zwart F A, van Teylingen C M and Natarajan A T (1994) Development of a cloning assay with high cloning efficiency to detect induction of 6-thiogua-nine-resistant lymphocytes in spleen of adult mice following in vivo inhalation exposure to 1, 3-butadiene, Mutat Res 309: 299-306. (Pubitemid 24268442)
17. van Dam F J, Natarajan A T and Tates A D (1992) Use of a T-lymphocyte clonal assay for determining HPRT mutant frequencies in individual rats, Mutat Res 271: 231-242.
18. Albertini R J (2001) HPRT mutations in humans: biomarkers for mechanistic studies, Mutation Research/Reviews in Mutation Research 489: 1-16. (Pubitemid 32973677)
19. Woodruff N W, Durant J L, Donhoffner L L, Penman B W and Crespi C L (2001) Human cell mutagenicity of chlorinated and unchlori-nated water and the disinfection byproduct 3-chloro-4-(dichloromethyl)-5-hy-droxy-2(5 H)-furanone (MX), Mutat Res 495: 157-168. (Pubitemid 32614193)
20. McPherson M, Taylor G and Quirke P (1991) PCR 1: A Practical Approach (Practical Approach S.), Oxford University Press, Oxford.
21. Gentest (1994) AHH-1 TK+/-Human Lymphoblastoid Cells. Routine cell culture, metabolite production and gene-locus mutation assay. Procedures for use. Gentest corporation, Woburn, MA 01801, USA.
22. DeMarini D M, Brock K H, Doerr C L and Moore M M (1988) Mutagenicity and clasto-genicity of proflavin in L5178Y/TK +/-3.7.2.C cells, Mutat Res 204: 323-328. (Pubitemid 18066094)
23. Doak S H, Jenkins G L, Johnson G E, Quick E, Parry E M and Parry J M (2007) Mechanistic influences for mutation induction curves after exposure to DNA-reactive carcinogens, Cancer Res 67: 3904-3911. (Pubitemid 46762179)
24. Zair Z M, Jenkins G J, Doak S H, Singh R, Brown K and Johnson G E (2011) N-Methylpurine DNA Glycosylase Plays a Pivotal Role in the Threshold Response of Ethyl Methanesulfonate-Induced Chromosome Damage. Toxicological Sciences, 119: 346-358.
25. Johnson G E, Quick E L, Parry E M and Parry J M (2010) Metabolic influences for mutation induction curves after exposure to Sudan-1 and para red. Mutagenesis, 25: 327-333.
26. Johnson G E, Doak S H, Griffiths S M, Quick E L, Skibinski D O F, Zaïr Z M and Jenkins G J (2009) Non-linear dose-response of DNA-reactive genotoxins: Recommendations for data analysis. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 678: 95-100.