Cpla 2α-mediated actin rearrangements downstream of the akt signaling is required for cronobacter sakazakii invasion into brain endothelial cells

Biochemical and Biophysical Research Communications

Volumn 417, Issue 3, 2012, Pages 925-930

  Liu, Dong Xin ^a   Zhao, Wei Dong ^a   Fang, Wen Gang ^a   Chen, Yu Hua ^a  

^a CHINA MEDICAL UNIVERSITY   (China)

Author keywords

Actin rearrangements; Akt; Brain endothelial cells; CPLA 2 ; Cronobacter sakazakii; Meningitis

Indexed keywords

ACTIN; CYTOSOLIC PHOSPHOLIPASE A2ALPHA; PHOSPHOLIPASE A2; PROTEIN KINASE B; SMALL INTERFERING RNA; UNCLASSIFIED DRUG; ACTIN DEPOLYMERIZING FACTOR; PHOSPHOLIPASE A2 GROUP IV; PLA2G4A PROTEIN, HUMAN;

ACTIN FILAMENT; ARTICLE; BRAIN CELL; CELL STIMULATION; CONTROLLED STUDY; CRONOBACTER SAKAZAKII; CRONOBACTER SAKAZAKII INFECTION; DEPOLYMERIZATION; ENTEROBACTERIACEAE INFECTION; ENZYME ACTIVATION; ENZYME PHOSPHORYLATION; GENE SILENCING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMMUNOFLUORESCENCE; MICROVASCULAR ENDOTHELIAL CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; BRAIN; CELL CULTURE; DRUG ANTAGONISM; METABOLISM; MICROBIOLOGY; MICROVASCULATURE; OPPORTUNISTIC INFECTION; PATHOGENICITY; VASCULAR ENDOTHELIUM; VASCULARIZATION;

ACTIN DEPOLYMERIZING FACTORS; ACTINS; BRAIN; CELLS, CULTURED; CRONOBACTER SAKAZAKII; ENDOTHELIUM, VASCULAR; ENTEROBACTERIACEAE INFECTIONS; GROUP IV PHOSPHOLIPASES A2; HUMANS; MICROVESSELS; OPPORTUNISTIC INFECTIONS; PROTO-ONCOGENE PROTEINS C-AKT; SIGNAL TRANSDUCTION;

EID: 84855989286     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2011.11.079     Document Type: Article

References (23)

1. Iversen C., Mullane N., McCardell B., Tall B.D., Lehner A., Fanning S., Stephan R., Joosten H. Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov. Int. J. Syst. Evol. Microbiol. 2008, 58:1442-1447.
2. Drudy D., Mullane N.R., Quinn T., Wall P.G., Fanning S. Enterobacter sakazakii: an emerging pathogen in powdered infant formula. Clin. Infect. Dis. 2006, 42:996-1002.
3. Lai K.K. Enterobacter sakazakii infections among neonates, infants, children, and adults. Case reports and a review of the literature. Medicine (Baltimore) 2001, 80:113-122.
4. Kim K.S. Mechanisms of microbial traversal of the blood-brain barrier. Nat. Rev. Microbiol. 2008, 6:625-634.
5. Li Q., Zhao W.D., Zhang K., Fang W.G., Hu Y., Wu S.H., Chen Y.H. PI3K-dependent host cell actin rearrangements are required for Cronobacter sakazakii invasion of human brain microvascular endothelial cells. Med. Microbiol. Immunol. 2010, 199:333-340.
6. Linkous A., Yazlovitskaya E. Cytosolic phospholipase A2 as a mediator of disease pathogenesis. Cell. Microbiol. 2010, 12:1369-1377.
7. Duan L., Gan H., Arm J., Remold H.G. Cytosolic phospholipase A2 participates with TNF-alpha in the induction of apoptosis of human macrophages infected with Mycobacterium tuberculosis H37Ra. J. Immunol. 2001, 166:7469-7476.
8. 2α in resident peritoneal macrophages by Listeria monocytogenes involves Listeriolysin O and TLR2. J. Biol. Chem. 2008, 283:4744-4755.
9. Mumy K.L., Bien J.D., Pazos M.A., Gronert K., Hurley B.P., McCormick B.A. Distinct isoforms of phospholipase A2 mediate the ability of Salmonella enterica serotype typhimurium and Shigella flexneri to induce the transepithelial migration of neutrophils. Infect. Immun. 2008, 76:3614-3627.
10. Das A., Asatryan L., Reddy M.A., Wass C.A., Stins M.F., Joshi S., Bonventre J.V., Kim K.S. Differential role of cytosolic phospholipase A2 in the invasion of brain microvascular endothelial cells by Escherichia coli and Listeria monocytogenes. J. Infect. Dis. 2001, 184:732-737.
11. Li M., Shang D.S., Zhao W.D., Tian L., Li B., Fang W.G., Zhu L., Man S.M., Chen Y.H. Amyloid beta interaction with receptor for advanced glycation end products up-regulates brain endothelial CCR5 expression and promotes T cells crossing the blood-brain barrier. J. Immunol. 2009, 182:5778-5788.
12. Singamsetty V.K., Wang Y., Shimada H., Prasadarao N.V. Outer membrane protein A expression in Enterobacter sakazakii is required to induce microtubule condensation in human brain microvascular endothelial cells for invasion. Microb. Pathog. 2008, 45:181-191.
13. Harteneck C., Frenzel H., Kraft R. N-(p-Amylcinnamoyl)anthranilic acid (ACA): a phospholipase A(2) inhibitor and TRP channel blocker. Cardiovasc. Drug Rev. 2007, 25:61-75.
14. 2 enzymes. Bioorg. Med. Chem. 2008, 16:10257-10269.
15. Ackermann E.J., Conde-Frieboes K., Dennis E.A. Inhibition of macrophage Ca(2+)-independent phospholipase A2 by bromoenol lactone and trifluoromethyl ketones. J. Biol. Chem. 1995, 270:445-450.
16. 2 α contributes to group B streptococcus penetration of the blood-brain barrier. Infect. Immun. 2011, 79:4088-4093.
17. Zhu L., Maruvada R., Sapirstein A., Malik K.U., Peters-Golden M., Kim K.S. Arachidonic acid metabolism regulates Escherichia coli penetration of the blood-brain barrier. Infect. Immun. 2010, 78:4302-4310.
18. Lin L.L., Wartmann M., Lin A.Y., Knopf J.L., Seth A., Davis R.J. CPLA2 is phosphorylated and activated by MAP kinase. Cell 1993, 72:269-278.
19. Kramer R.M., Roberts E.F., Um S.L., Borsch-Haubold A.G., Watson S.P., Fisher M.J., Jakubowski J.A. p38 mitogen-activated protein kinase phosphorylates cytosolic phospholipase A2 (cPLA2) in thrombin-stimulated platelets. Evidence that proline-directed phosphorylation is not required for mobilization of arachidonic acid by cPLA2. J. Biol. Chem. 1996, 271:27723-27729.
20. Townsend S.M., Hurrell E., Gonzalez-Gomez I., Lowe J., Frye J.G., Forsythe S., Badger J.L. Enterobacter sakazakii invades brain capillary endothelial cells, persists in human macrophages influencing cytokine secretion and induces severe brain pathology in the neonatal rat. Microbiology 2007, 153:3538-3547.
21. Pace J., Hayman M.J., Galan J.E. Signal transduction and invasion of epithelial cells by S. typhimurium. Cell 1993, 72:505-514.
22. Moes M., Boonstra J., Regan-Klapisz E. Novel role of cPLA(2)alpha in membrane and actin dynamics. Cell. Mol. Life Sci. 2010, 67:1547-1557.
23. Kakinuma N., Roy B.C., Zhu Y., Wang Y., Kiyama R. Kank regulates RhoA-dependent formation of actin stress fibers and cell migration via 14-3-3 in PI3K-Akt signaling. J. Cell Biol. 2008, 181:537-549.