Isolation and culture of human astrocytes

Methods in Molecular Biology

Volumn 814, Issue , 2012, Pages 137-151

  Sharif, Ariane ^a   Prevot, Vincent ^a  

^a UNIV LILLE   (France)

Author keywords

Adult; Astrocyte; Cerebral cortex; EGF related ligands; Fetus; Human; Hypothalamus; Immunostaining; Morphology; Proliferation

Indexed keywords

ADULT; ARTICLE; ASTROCYTE; BRAIN CORTEX; CELL PROLIFERATION; CULTURE TECHNIQUE; CYTOLOGY; FETUS; HUMAN; HYPOTHALAMUS; IMMUNOHISTOCHEMISTRY; METHODOLOGY; NERVE CELL PLASTICITY; PHYSIOLOGY;

ADULT; ASTROCYTES; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CEREBRAL CORTEX; FETUS; HUMANS; HYPOTHALAMUS; IMMUNOHISTOCHEMISTRY; NEURONAL PLASTICITY;

RODENTIA;

EID: 84855974865     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-452-0_11     Document Type: Article

References (9)

1. Wang, D. D., Bordey, A. (2008) The astrocyte odyssey. Prog Neurobiol 86, 342-367.
2. Oberheim, N. A., Takano, T., Han, X., He, W., Lin, J. H., Wang, F., Xu, Q., Wyatt, J. D., Pilcher, W., Ojemann, J. G., Ransom, B. R., Goldman, S. A., and Nedergaard, M. (2009) Uniquely hominid features of adult human astrocytes. J Neurosci 29, 3276-87.
3. Sharif, A., Duhem-Tonnelle, V., Allet, C., Baroncini, M., Loyens, A., Kerr-Conte, J., Collier, F., Blond, S., Ojeda, S. R., Junier, M. P., and Prevot, V. (2009) Differential erbB signaling in astrocytes from the cerebral cortex and the hypothalamus of the human brain. Glia 57, 362-79.
4. Zelenaia, O., Schlag, B. D., Gochenauer, G. E., Ganel, R., Song, W., Beesley, J. S., Grinspan, J. B., Rothstein, J. D., and Robinson, M. B. (2000) Epidermal growth factor receptor agonists increase expression of glutamate transporter GLT-1 in astrocytes through pathways dependent on phosphatidylinositol 3-kinase and transcription factor NF-kappaB. Mol Pharmacol 57, 667-78. (Pubitemid 30183705)
5. Zhou, R., Wu, X., and Skalli, O. (2001) TGFalpha induces a stationary, radial-glia like phenotype in cultured astrocytes. Brain Res Bull 56, 37-42. (Pubitemid 32962936)
6. Sharif, A., Legendre, P., Prevot, V., Allet, C., Romao, L., Studler, J. M., Chneiweiss, H., and Junier, M. P. (2007) Transforming growth factor alpha promotes sequential conversion of mature astrocytes into neural progenitors and stem cells. Oncogene 26, 2695-706. (Pubitemid 46663827)
7. Sharif, A., Prevot, V., Renault-Mihara, F., Allet, C., Studler, J. M., Canton, B., Chneiweiss, H., and Junier, M. P. (2006) Transforming growth factor alpha acts as a gliatrophin for mouse and human astrocytes. Oncogene 25, 4076-85. (Pubitemid 44024870)
8. Junier, M. P. (2000) What role(s) for TGFalpha in the central nervous system? Prog Neurobiol 62, 443-73.
9. Rugh, R. (1964) Vertebrate embryology: the dynamics of development. Harcourt, Brace & World, New York.