A new bacterial co-expression system for over-expressing soluble protein and validating protein-protein interaction

Methods in Molecular Biology

Volumn 824, Issue , 2012, Pages 235-249

  Zeng, Jumei ^a   He, Zheng Guo ^a  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

Co expression; pGEX; pHEX; Protein complexes

Indexed keywords

CARRIER PROTEIN; MULTIPROTEIN COMPLEX; OLIGONUCLEOTIDE; RECOMBINANT PROTEIN;

ARTICLE; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; NUCLEIC ACID AMPLIFICATION; PHYSIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN FOLDING; PROTEIN STABILITY; SOLUBILITY; TWO HYBRID SYSTEM; WESTERN BLOTTING;

BLOTTING, WESTERN; CARRIER PROTEINS; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; MULTIPROTEIN COMPLEXES; NUCLEIC ACID AMPLIFICATION TECHNIQUES; OLIGONUCLEOTIDES; PROTEIN FOLDING; PROTEIN STABILITY; RECOMBINANT PROTEINS; SOLUBILITY; TWO-HYBRID SYSTEM TECHNIQUES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84855964716     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-433-9_12     Document Type: Article

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