Oxidative stress and cytotoxicity generated by dental composites in human pulp cells

Clinical Oral Investigations

Volumn 16, Issue 1, 2012, Pages 215-224

  Krifka, Stephanie ^a   Seidenader, Claudia ^a   Hiller, Karl Anton ^a   Schmalz, Gottfried ^a   Schweikl, Helmut ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

Cytotoxicity; Dental pulp cells; Reactive oxygen species; Silorane; TEGDMA and HEMA free composites

Indexed keywords

2 HYDROXYETHYL METHACRYLATE; 2,2 BIS (4 (2 METHACRYLOXYETHOXY)PHENYL)PROPANE; 2,2-BIS-(4-(2-METHACRYLOXYETHOXY)PHENYL)PROPANE; BIOMEDICAL AND DENTAL MATERIALS; BISPHENOL A BIS(2 HYDROXYPROPYL) ETHER DIMETHACRYLATE; COLORING AGENT; CRYSTAL VIOLET; DIAGNOSTIC AGENT; FILTEK SILORANE; GLASS IONOMER; HERMES COMPOSITE RESIN; MACROGOL DERIVATIVE; METHACRYLIC ACID DERIVATIVE; POLYESTER; POLYMETHACRYLIC ACID DERIVATIVE; REACTIVE OXYGEN METABOLITE; RESIN; RESIN CEMENT; SILOXANE; SOLITAIRE 2 COMPOSITE RESIN; TETRIC CERAM; TETRIC EVOCERAM; TRIETHYLENE GLYCOL DIMETHACRYLATE; VITRABOND;

ARTICLE; CELL CULTURE; CELL SURVIVAL; CHEMISTRY; COMPARATIVE STUDY; CULTURE TECHNIQUE; CYTOLOGY; DRUG EFFECT; FLOW CYTOMETRY; HUMAN; OXIDATIVE STRESS; PHYSIOLOGY; TOOTH PULP;

BISPHENOL A-GLYCIDYL METHACRYLATE; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CELLS, CULTURED; COLORING AGENTS; COMPOSITE RESINS; DENTAL MATERIALS; DENTAL PULP; FLOW CYTOMETRY; GENTIAN VIOLET; GLASS IONOMER CEMENTS; HUMANS; METHACRYLATES; OXIDATIVE STRESS; POLYESTERS; POLYETHYLENE GLYCOLS; POLYMETHACRYLIC ACIDS; REACTIVE OXYGEN SPECIES; RESIN CEMENTS; SILOXANES;

EID: 84855933162     PISSN: 14326981     EISSN: 14363771     Source Type: Journal    
DOI: 10.1007/s00784-010-0508-5     Document Type: Article

References (40)

1. Sloan AJ, Smith AJ (2007) Stem cells and the dental pulp: potential roles in dentine regeneration and repair. Oral Dis 13: 151-157.
2. Smith AJ, Cassidy N, Perry H, Begue-Kirn C, Ruch JV, Lesot H (1995) Reactionary dentinogenesis. Int J Dev Biol 39: 273-280.
3. Ferracane JL, Cooper PR, Smith AJ (2010) Can interaction of materials with the dentin-pulp complex contribute to dentin regeneration? Odontology 98: 2-14.
4. Seiss M, Langer C, Hickel R, Reichl FX (2009) Quantitative determination of TEGDMA, BHT, and DMABEE in eluates from polymerized resin-based dental restorative materials by use of GC/MS. Arch Toxicol 83: 1109-1115.
5. Shawkat ES, Shortall AC, Addison O, Palin WM (2009) Oxygen inhibition and incremental layer bond strengths of resin composites. Dent Mater 25: 1338-1346.
6. Rueggeberg FA, Margeson DH (1990) The effect of oxygen inhibition on an unfilled/filled composite system. J Dent Res 69: 1652-1658.
7. Noda M, Wataha JC, Kaga M, Lockwood PE, Volkmann KR, Sano H (2002) Components of dentinal adhesives modulate heat shock protein 72 expression in heat-stressed THP-1 human monocytes at sublethal concentrations. J Dent Res 81: 265-269.
8. Michelsen VB, Moe G, Skalevik R, Jensen E, Lygre H (2007) Quantification of organic eluates from polymerized resin-based dental restorative materials by use of GC/MS. J Chromatogr B Analyt Technol Biomed Life Sci 850: 83-91.
9. Schweikl H, Hiller KA, Eckhardt A, Bolay C, Spagnuolo G, Stempfl T, Schmalz G (2008) Differential gene expression involved in oxidative stress response caused by triethylene glycol dimethacrylate. Biomaterials 29: 1377-1387.
10. Spagnuolo G, D'Anto V, Cosentino C, Schmalz G, Schweikl H, Rengo S (2006) Effect of N-acetyl-L-cysteine on ROS production and cell death caused by HEMA in human primary gingival fibroblasts. Biomaterials 27: 1803-1809.
11. Engelmann J, Volk J, Leyhausen G, Geurtsen W (2005) ROS formation and glutathione levels in human oral fibroblasts exposed to TEGDMA and camphorquinone. J Biomed Mater Res B Appl Biomater 75: 272-276.
12. Matsuzawa A, Ichijo H (2005) Stress-responsive protein kinases in redox-regulated apoptosis signaling. Antioxid Redox Signal 7: 472-481.
13. Lefeuvre M, Bourd K, Loriot MA, Goldberg M, Beaune P, Perianin A, Stanislawski L (2004) TEGDMA modulates glutathione transferase P1 activity in gingival fibroblasts. J Dent Res 83: 914-919.
14. Schweikl H, Hartmann A, Hiller KA, Spagnuolo G, Bolay C, Brockhoff G, Schmalz G (2007) Inhibition of TEGDMA and HEMA-induced genotoxicity and cell cycle arrest by N-acetylcysteine. Dent Mater 23: 688-695.
15. Smith AJ (2002) Pulpal responses to caries and dental repair. Caries Res 36: 223-232.
16. Spagnuolo G, D'Anto V, Valletta R, Strisciuglio C, Schmalz G, Schweikl H, Rengo S (2008) Effect of 2-hydroxyethyl methacrylate on human pulp cell survival pathways ERK and AKT. J Endod 34: 684-688.
17. Galler KM, Schweikl H, Hiller KA, Cavender AC, Bolay C, D'Souza RN, Schmalz G. TEGDMA reduces mineralization in dental pulp cells. J Dent Res, in press.
18. Weinmann W, Thalacker C, Guggenberger R (2005) Siloranes in dental composites. Dent Mater 21: 68-74.
19. ISO 10993-12: 2007. Biological evaluation of medical devices - Part 12: Sample preparation and reference materials. International Organisation for Standardization, Case postale 56, CH-1211 Geneva 20, Switzerland.
20. Myhre O, Andersen JM, Aarnes H, Fonnum F (2003) Evaluation of the probes 2', 7'-dichlorofluorescin diacetate, luminol, and lucigenin as indicators of reactive species formation. Biochem Pharmacol 65: 1575-1582.
21. Galler K, Schweikl H, Thonemann B, Schmalz G (2006) Human pulp-derived cells immortalized with SV40. Eur J Oral Sci 114: 138-146.
22. Gilles RJ, Didier N, Denton M (1986) Determination of cell number in monolayer cultures. Anal Biochem 159: 109-113.
23. Forman HJ, Fukuto JM, Torres M (2004) Redox signaling: thiol chemistry defines which reactive oxygen and nitrogen species can act as second messengers. Am J Physiol Cell Physiol 287: C246-C256.
24. Stanislawski L, Lefeuvre M, Bourd K, Soheili-Majd E, Goldberg M, Perianin A (2003) TEGDMA-induced toxicity in human fibroblasts is associated with early and drastic glutathione depletion with subsequent production of oxygen reactive species. J Biomed Mater Res A 66: 476-482.
25. Engelmann J, Janke V, Volk J, Leyhausen G, von NN, Schlegelberger B, Geurtsen W (2004) Effects of BisGMA on glutathione metabolism and apoptosis in human gingival fibroblasts in vitro. Biomaterials 25: 4573-4580.
26. Nocca G, D'Antò V, Desiderio C, Rossetti DV, Valletta R, Baquala AM, Schweikl H, Lupi A, Rengo S, Spagnuolo G (2010) N-acetyl cysteine directed detoxification of 2-hydroxyethyl methacrylate by adduct formation. Biomaterials 31: 2508-2516.
27. Paranjpe A, Cacalano NA, Hume WR, Jewett A (2009) N-acetyl cysteine mediates protection from 2-hydroxyethyl methacrylate induced apoptosis via nuclear factor kappa B-dependent and independent pathways: potential involvement of JNK. Toxicol Sci 108: 356-366.
28. Krifka S, Petzel C, Hiller KA, Frank EM, Bosl C, Spagnuolo G, Reichl FX, Schmalz G, Schweikl H (2010) Resin monomer-induced differential activation of MAP kinases and apoptosis in mouse macrophages and human pulp cells. Biomaterials 31: 2964-2975.
29. LeBel CP, Ishiropoulos H, Bondy SC (1992) Evaluation of the probe 2, 7-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress. Chem Res Toxicol 5: 227-231.
30. Demirci M, Hiller KA, Bosl C, Galler K, Schmalz G, Schweikl H (2008) The induction of oxidative stress, cytotoxicity, and genotoxicity by dental adhesives. Dent Mater 24: 362-371.
31. Spagnuolo G, Galler K, Schmalz G, Cosentino C, Rengo S, Schweikl H (2004) Inhibition of phosphatidylinositol 3-kinase amplifies TEGDMA-induced apoptosis in primary human pulp cells. J Dent Res 83: 703-707.
32. Schweikl H, Spagnuolo G, Schmalz G (2006) Genetic and cellular toxicology of dental resin monomers. J Dent Res 85: 870-877.
33. Schweikl H, Hiller KA, Bolay C, Kreissl M, Kreismann W, Nusser A, Steinhauser S, Wieczorek J, Vasold R, Schmalz G (2005) Cytotoxic and mutagenic effects of dental composite materials. Biomaterials 26: 1713-1719.
34. Michelsen VB, Moe G, Strøm MB, Jensen E, Lygre H (2008) Quantitative analysis of TEGDMA and HEMA eluted into saliva from two dental composites by use of GC/MS and tailor-made internal standards. Dent Mater 24: 724-731.
35. Wataha JC, Lockwood PE, Bouillaguet S, Noda M (2003) In vitro biological response to core and flowable dental restorative materials. Dent Mater 19: 25-31.
36. Al-Hiyasat AS, Darmani H, Milhem MM (2005) Cytotoxicity evaluation of dental resin composites and their flowable derivatives. Clin Oral Investig 9: 21-25.
37. Kopperud HM, Schmidt M, Kleven IS (2010) Elution of substances from a silorane-based dental composite. Eur J Oral Sci 118: 100-102.
38. Schweikl H, Schmalz G, Weinmann W (2004) The induction of gene mutations and micronuclei by oxiranes and siloranes in mammalian cells in vitro. J Dent Res 83: 17-21.
39. Schweikl H, Schmalz G, Weinmann W (2002) Mutagenic activity of structurally related oxiranes and siloranes in Salmonella typhimurium. Mutat Res 521: 19-27.
40. Brackett MG, Bouillaguet S, Lockwood PE, Rotenberg S, Lewis JB, Messer RL, Wataha JC (2007) In vitro cytotoxicity of dental composites based on new and traditional polymerization chemistries. J Biomed Mater Res B Appl Biomater 81: 397-402.