Isolation of undifferentiated and early differentiating type a spermatogonia from Pou5f1-GFP reporter mice

Methods in Molecular Biology

Volumn 825, Issue , 2012, Pages 31-44

  Garcia, Thomas ^a   Hofmann, Marie Claude ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

Fluorescence activated cell sorting; Green fluorescent protein; Pou5f1; Reporter mice; Undifferentiated spermatogonia

Indexed keywords

GREEN FLUORESCENT PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; POU5F1 PROTEIN, MOUSE; STEM CELL FACTOR RECEPTOR;

ANIMAL; ARTICLE; CELL DIFFERENTIATION; CELL SEPARATION; CYTOLOGY; FLOW CYTOMETRY; GENETICS; MALE; METABOLISM; METHODOLOGY; MOUSE; REPORTER GENE; SPERMATOGENESIS; SPERMATOGONIUM; TRANSGENIC MOUSE;

ANIMALS; CELL DIFFERENTIATION; CELL SEPARATION; FLOW CYTOMETRY; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; MALE; MICE; MICE, TRANSGENIC; OCTAMER TRANSCRIPTION FACTOR-3; PROTO-ONCOGENE PROTEINS C-KIT; SPERMATOGENESIS; SPERMATOGONIA;

MUS;

EID: 84855888048     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-436-0_3     Document Type: Article

References (29)

1. Nicholas CR, Xu EY, Banani SF, Hammer RE, Hamra FK, and Reijo Pera RA (2009) Characterization of a Dazl-GFP germ cell-specific reporter. Genesis 47: 74-84
2. Yoshida S, Takakura A, Ohbo K, Abe K, Wakabayashi J, Yamamoto M, Suda T, and Nabeshima Y (2004) Neurogenin3 delineates the earliest stages of spermatogenesis in the mouse testis. Dev Biol 269: 447-458 (Pubitemid 38521110)
3. Nayernia K, Li M, Jaroszynski L, Khusainov R, Wulf G, Schwandt I, Korabiowska M, Michelmann HW, Meinhardt A, and Engel W (2004) Stem cell based therapeutical approach of male infertility by teratocarcinoma derived germ cells. Hum Mol Genet 13: 1451-1460 (Pubitemid 39023053)
4. Ohbo K, Yoshida S, Ohmura M, Ohneda O, Ogawa T, Tsuchiya H, Kuwana T, Kehler J, Abe K, Scholer HR, and Suda T (2003) Identification and characterization of stem cells in prepubertal spermatogenesis in mice small star, fi lled. Dev Biol 258: 209-225 (Pubitemid 36627667)
5. Yeom YI, Fuhrmann G, Ovitt CE, Brehm A, Ohbo K, Gross M, Hubner K, and Scholer HR (1996) Germline regulatory element of Oct-4 specific for the totipotent cycle of embryonal cells. Development 122: 881-894 (Pubitemid 26095994)
6. Yoshimizu T, Sugiyama N, De Felice M, Yeom YI, Ohbo K, Masuko K, Obinata M, Abe K, Scholer HR, and Matsui Y (1999) Germline specific expression of the Oct-4/green fluorescent protein (GFP) transgene in mice. Dev Growth Differ 41: 675-684 (Pubitemid 30026664)
7. Pesce M, Wang X, Wolgemuth DJ, and Scholer H (1998) Differential expression of the Oct-4 transcription factor during mouse germ cell differentiation. Mech Dev 71: 89-98 (Pubitemid 28153521)
8. Tadokoro Y, Yomogida K, Ohta H, Tohda A, and Nishimune Y (2002) Homeostatic regulation of germinal stem cell proliferation by the GDNF/FSH pathway. Mech Dev 113: 29-39 (Pubitemid 34233722)
9. Schrans-Stassen BH, van de Kant HJ, de Rooij DG, and van Pelt AM (1999) Differential expression of c-kit in mouse undifferentiated and differentiating type A spermatogonia. Endocrinology 140: 5894-5900 (Pubitemid 30645378)
10. Izadyar F, Pau F, Marh J, Slepko N, Wang T, Gonzalez R, Ramos T, Howerton K, Sayre C, and Silva F (2008) Generation of multipotent cell lines from a distinct population of male germ line stem cells. Reproduction 135: 771-784 (Pubitemid 351837390)
11. Shinohara T, Orwig KE, Avarbock MR, and Brinster RL (2000) Spermatogonial stem cell enrichment by multiparameter selection of mouse testis cells. Proc Natl Acad Sci USA 97: 8346-8351 (Pubitemid 30639675)
12. Boiani M, Kehler J, and Scholer HR (2004) Activity of the germline-specific Oct4-GFP transgene in normal and clone mouse embryos. Methods Mol Biol 254: 1-34
13. Hammond SS, and Matin A (2009) Tools for the genetic analysis of germ cells. Genesis 47: 617-627
14. Szabo PE, Hubner K, Scholer H, and Mann JR (2002) Allele-specific expression of imprinted genes in mouse migratory primordial germ cells. Mech Dev 115: 157-160 (Pubitemid 34615682)
15. Lengner CJ, Camargo FD, Hochedlinger K, Welstead GG, Zaidi S, Gokhale S, Scholer HR, Tomilin A, and Jaenisch R (2007) Oct4 expression is not required for mouse somatic stem cell self-renewal. Cell Stem Cell 1: 403-415 (Pubitemid 47506536)
16. Ohmura M, Yoshida S, Ide Y, Nagamatsu G, Suda T, and Ohbo K (2004) Spatial analysis of germ stem cell development in Oct-4/ EGFP transgenic mice. Arch Histol Cytol 67: 285-296 (Pubitemid 40215902)
17. Garrick D, Fiering S, Martin DI, and Whitelaw E (1998) Repeat-induced gene silencing in mammals. Nat Genet 18: 56-59 (Pubitemid 28027880)
18. Garrick D, Sutherland H, Robertson G, and Whitelaw E (1996) Variegated expression of a globin transgene correlates with chromatin accessibility but not methylation status. Nucleic Acids Res 24: 4902-4909 (Pubitemid 27007894)
19. Henikoff S (1998) Conspiracy of silence among repeated transgenes. Bioessays 20: 532-535 (Pubitemid 28362298)
20. Meissner A, Wernig M, and Jaenisch R (2007) Direct reprogramming of genetically unmodified fi broblasts into pluripotent stem cells. Nat Biotechnol 25: 1177-1181 (Pubitemid 47538112)
21. Chen S, Do JT, Zhang Q, Yao S, Yan F, Peters EC, Scholer HR, Schultz PG, and Ding S (2006) Self-renewal of embryonic stem cells by a small molecule. Proc Natl Acad Sci USA 103: 17266-17271 (Pubitemid 44788958)
22. Guan K, Wolf F, Becker A, Engel W, Nayernia K, and Hasenfuss G (2009) Isolation and cultivation of stem cells from adult mouse testes. Nat Protoc 4: 143-154
23. Hofmann MC, Braydich-Stolle L, and Dym M (2005) Isolation of male germ-line stem cells; influence of GDNF. Dev Biol 279: 114-124 (Pubitemid 40222173)
24. Kanatsu-Shinohara M, Ogonuki N, Inoue K, Miki H, Ogura A, Toyokuni S, and Shinohara T (2003) Long-term proliferation in culture and germline transmission of mouse male germline stem cells. Biol Reprod 69: 612-616 (Pubitemid 36885967)
25. Kubota H, Avarbock MR, and Brinster RL (2004) Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells. Proc Natl Acad Sci USA 101: 16489-16494 (Pubitemid 39557740)
26. Vincent S, Segretain D, Nishikawa S, Nishikawa SI, Sage J, Cuzin F, and Rassoulzadegan M (1998) Stage-specific expression of the Kit receptor and its ligand (KL) during male gametogenesis in the mouse: a Kit-KL interaction critical for meiosis. Development 125: 4585-4593 (Pubitemid 28564301)
27. Kubota H, Avarbock MR, and Brinster RL (2004) Culture conditions and single growth factors affect fate determination of mouse spermatogonial stem cells. Biol Reprod 71: 722-731 (Pubitemid 39108631)
28. Bellve AR, Cavicchia JC, Millette CF, O'Brien DA, Bhatnagar YM, and Dym M (1977) Spermatogenic cells of the prepuberal mouse. Isolation and morphological characterization. J Cell Biol 74: 68-85 (Pubitemid 8141486)
29. de Jonge HJ, Fehrmann RS, de Bont ES, Hofstra RM, Gerbens F, Kamps WA, de Vries EG, van der Zee AG, te Meerman GJ, and ter Elst A (2007) Evidence based selection of housekeeping genes. PLoS One 2: e898