A continuous spectrophotometric assay for mitogen-activated protein kinase kinases

Analytical Biochemistry

Volumn 421, Issue 1, 2012, Pages 191-197

  Zheng, Li Sha ^a   Zhang, Yuan Yuan ^b   Wu, Jia Wei ^c   Wu, Zhenguo ^d   Zhang, Zhong Yin ^e   Wang, Zhi Xin ^b,c  

^a BEIHANG UNIVERSITY   (China)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^c TSINGHUA UNIVERSITY   (China)

^d HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Hong Kong)

^e INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Dephosphorylation; MAP kinase; MAP kinase kinase; MAP kinase phosphatase; Phosphorylation

Indexed keywords

BINDING ENERGY; BIOMOLECULES; PHOSPHATASES; PHOSPHORYLATION; SPECTROPHOTOMETERS; SPECTROPHOTOMETRY;

DEPHOSPHORYLATIONS; MAP KINASE; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN-ACTIVATED PROTEIN KINASE KINASE; PURINE NUCLEOSIDE PHOSPHORYLASE; SPECTRO-PHOTOMETRIC METHOD; SPECTROPHOTOMETRIC ASSAYS; SUBMICROMOLAR CONCENTRATIONS;

SUBSTRATES;

2 AMINO 6 MERCAPTO 7 METHYL PURINE RIBONUCLEOSIDE; 2 AMINO 6 MERCAPTO 7 METHYLPURINE; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE KINASE 6; PURINE NUCLEOSIDE DERIVATIVE; PURINE NUCLEOSIDE PHOSPHORYLASE; RIBOSE 1 PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME MECHANISM; PRIORITY JOURNAL; PROTEIN DEPHOSPHORYLATION; PROTEIN PHOSPHORYLATION; PROTEIN STRUCTURE; SPECTROPHOTOMETRY; STEADY STATE;

EID: 84855885984     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.11.018     Document Type: Article

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